The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil

Abstract. Alami NH, Maulidiya LL, Kuswytasari ND, Zulaika E, Shovitri M. 2019. The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil. Biodiversitas 20: 1833-1838. Bioremediation of crude oil contaminated soil eventually depends on the activities of m...

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Autores principales: NUR HIDAYATUL ALAMI, LUTHFI LAILATUL MAULIDIYA, N D KUSWYTASARI, ENNY ZULAIKA, MAYA SHOVITRI
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Publicado: MBI & UNS Solo 2019
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spelling oai:doaj.org-article:414987038a134da9af80c218a366f1802021-11-21T21:42:27ZThe potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil1412-033X2085-472210.13057/biodiv/d200707https://doaj.org/article/414987038a134da9af80c218a366f1802019-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3499https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Alami NH, Maulidiya LL, Kuswytasari ND, Zulaika E, Shovitri M. 2019. The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil. Biodiversitas 20: 1833-1838. Bioremediation of crude oil contaminated soil eventually depends on the activities of microorganisms to degrade contaminants. Bioaugmentation, through the addition of microbial culture to contaminated soil, can be used to enhance degradation rates. This research aimed to determine the effect of yeast formulations isolated from the oil-contaminated soil and mangrove rhizosphere to degrade crude oil and evaluate the most effective yeast formulation and incubation timefor crude oil degradation. This research was conducted by subculturing yeast in the slant agar medium, screening hydrocarbonoclastic yeast in crude oil- Bushnell Hass Mineral Salt (BHMS) medium, biodegradation test, measuring the total cell number with Total Plate Count (TPC), Total Petroleum Hydrocarbon (TPH) test with the gravimetric method, and measuring soil moisture content. The design of the research was Completely Randomized Factorial Design with 3 replications. Data were analyzed quantitatively by using statistical tests for TPC and TPH, while a descriptive qualitative test analyzed soil moisture. The result showed that yeast formulation and incubation time affected crude oil degradation. An effective yeast consortium for oil degradation is T20 consortium with an incubation period of 30 days, which result in 72.26 % oil degradation.NUR HIDAYATUL ALAMILUTHFI LAILATUL MAULIDIYAN D KUSWYTASARIENNY ZULAIKAMAYA SHOVITRIMBI & UNS Soloarticlecrude oil, degradation, mangrove rhizosphere, soil, yeast consortiumBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 7 (2019)
institution DOAJ
collection DOAJ
language EN
topic crude oil, degradation, mangrove rhizosphere, soil, yeast consortium
Biology (General)
QH301-705.5
spellingShingle crude oil, degradation, mangrove rhizosphere, soil, yeast consortium
Biology (General)
QH301-705.5
NUR HIDAYATUL ALAMI
LUTHFI LAILATUL MAULIDIYA
N D KUSWYTASARI
ENNY ZULAIKA
MAYA SHOVITRI
The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
description Abstract. Alami NH, Maulidiya LL, Kuswytasari ND, Zulaika E, Shovitri M. 2019. The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil. Biodiversitas 20: 1833-1838. Bioremediation of crude oil contaminated soil eventually depends on the activities of microorganisms to degrade contaminants. Bioaugmentation, through the addition of microbial culture to contaminated soil, can be used to enhance degradation rates. This research aimed to determine the effect of yeast formulations isolated from the oil-contaminated soil and mangrove rhizosphere to degrade crude oil and evaluate the most effective yeast formulation and incubation timefor crude oil degradation. This research was conducted by subculturing yeast in the slant agar medium, screening hydrocarbonoclastic yeast in crude oil- Bushnell Hass Mineral Salt (BHMS) medium, biodegradation test, measuring the total cell number with Total Plate Count (TPC), Total Petroleum Hydrocarbon (TPH) test with the gravimetric method, and measuring soil moisture content. The design of the research was Completely Randomized Factorial Design with 3 replications. Data were analyzed quantitatively by using statistical tests for TPC and TPH, while a descriptive qualitative test analyzed soil moisture. The result showed that yeast formulation and incubation time affected crude oil degradation. An effective yeast consortium for oil degradation is T20 consortium with an incubation period of 30 days, which result in 72.26 % oil degradation.
format article
author NUR HIDAYATUL ALAMI
LUTHFI LAILATUL MAULIDIYA
N D KUSWYTASARI
ENNY ZULAIKA
MAYA SHOVITRI
author_facet NUR HIDAYATUL ALAMI
LUTHFI LAILATUL MAULIDIYA
N D KUSWYTASARI
ENNY ZULAIKA
MAYA SHOVITRI
author_sort NUR HIDAYATUL ALAMI
title The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
title_short The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
title_full The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
title_fullStr The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
title_full_unstemmed The potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
title_sort potential of yeasts from the oil-contaminated soil and mangrove rhizosphere for degrading crude oil
publisher MBI & UNS Solo
publishDate 2019
url https://doaj.org/article/414987038a134da9af80c218a366f180
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