MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration

Background: ncRNAs have been identified as oncogenic drivers and tumor suppressors in any type of cancer. Although many classes of ncRNAs have been reported, most studies have been performed on microRNAs (miRNAs). miRNAs can regulate several target genes and affect important processes such as homeos...

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Autores principales:	Seyed Omar Ebrahimi, Somayeh Reiisi
Formato:	article
Lenguaje:	EN FA
Publicado:	Bushehr University of Medical Sciences 2021
Materias:	mir-6165 breast cancer oncomir cell proliferation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/414da5339c0a4466bfc0a694632516e2
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spelling	oai:doaj.org-article:414da5339c0a4466bfc0a694632516e22021-12-04T05:40:39ZMiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration1735-43741735-6954https://doaj.org/article/414da5339c0a4466bfc0a694632516e22021-11-01T00:00:00Zhttp://ismj.bpums.ac.ir/article-1-1514-en.htmlhttps://doaj.org/toc/1735-4374https://doaj.org/toc/1735-6954Background: ncRNAs have been identified as oncogenic drivers and tumor suppressors in any type of cancer. Although many classes of ncRNAs have been reported, most studies have been performed on microRNAs (miRNAs). miRNAs can regulate several target genes and affect important processes such as homeostasis, angiogenesis, cell proliferation, differentiation, and apoptosis. Located in the p75NTR gene, miR-6165 is known to induce apoptosis in colorectal cell lines, and one study proposed a tumor suppressive role in colorectal cancer. However, its mechanism of action in breast cancer is not completely understood yet. Therefore, this study aimed to consider the expression level and the effect of miR-6165 on the proliferation and migration in breast cancer. Material and methods: Fifty tumor and adjacent non-tumor tissues were examined in the study. miR-6165 levels were evaluated by qPCR in breast cancer cell lines and tumor tissues. Pre-mir-6165 was cloned in the pEGFPN1 vector. Next, human breast cancer MCF7 cells were cultured and pre-miR-6165 vector was transfected to breast cancer line. The effects on cell proliferation and migration were investigated with MTT assay and scratch test, respectively. Bioinformatics analysis was performed through enrichment and hub genes finding for miRNA targets. Results: miR-6165 was overexpressed in breast cancer tumor tissues and cell lines. High expression of miR-6165 was directly related to the metastasis. miR-6165 increased proliferation and migration in breast cancer cell line. Conclusions: miR-6165 may function as an oncomir and increase the growth and migration of cells which may consequently serve as a therapeutic goal for breast cancer.Seyed Omar EbrahimiSomayeh ReiisiBushehr University of Medical Sciencesarticlemir-6165breast canceroncomircell proliferationMedicine (General)R5-920ENFAIranian South Medical Journal , Vol 24, Iss 5, Pp 439-453 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	mir-6165 breast cancer oncomir cell proliferation Medicine (General) R5-920
spellingShingle	mir-6165 breast cancer oncomir cell proliferation Medicine (General) R5-920 Seyed Omar Ebrahimi Somayeh Reiisi MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
description	Background: ncRNAs have been identified as oncogenic drivers and tumor suppressors in any type of cancer. Although many classes of ncRNAs have been reported, most studies have been performed on microRNAs (miRNAs). miRNAs can regulate several target genes and affect important processes such as homeostasis, angiogenesis, cell proliferation, differentiation, and apoptosis. Located in the p75NTR gene, miR-6165 is known to induce apoptosis in colorectal cell lines, and one study proposed a tumor suppressive role in colorectal cancer. However, its mechanism of action in breast cancer is not completely understood yet. Therefore, this study aimed to consider the expression level and the effect of miR-6165 on the proliferation and migration in breast cancer. Material and methods: Fifty tumor and adjacent non-tumor tissues were examined in the study. miR-6165 levels were evaluated by qPCR in breast cancer cell lines and tumor tissues. Pre-mir-6165 was cloned in the pEGFPN1 vector. Next, human breast cancer MCF7 cells were cultured and pre-miR-6165 vector was transfected to breast cancer line. The effects on cell proliferation and migration were investigated with MTT assay and scratch test, respectively. Bioinformatics analysis was performed through enrichment and hub genes finding for miRNA targets. Results: miR-6165 was overexpressed in breast cancer tumor tissues and cell lines. High expression of miR-6165 was directly related to the metastasis. miR-6165 increased proliferation and migration in breast cancer cell line. Conclusions: miR-6165 may function as an oncomir and increase the growth and migration of cells which may consequently serve as a therapeutic goal for breast cancer.
format	article
author	Seyed Omar Ebrahimi Somayeh Reiisi
author_facet	Seyed Omar Ebrahimi Somayeh Reiisi
author_sort	Seyed Omar Ebrahimi
title	MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
title_short	MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
title_full	MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
title_fullStr	MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
title_full_unstemmed	MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration
title_sort	mir-6165 dysregulation in breast cancer and its effect on cell proliferation and migration
publisher	Bushehr University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/414da5339c0a4466bfc0a694632516e2
work_keys_str_mv	AT seyedomarebrahimi mir6165dysregulationinbreastcanceranditseffectoncellproliferationandmigration AT somayehreiisi mir6165dysregulationinbreastcanceranditseffectoncellproliferationandmigration
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MiR-6165 Dysregulation in Breast Cancer and Its Effect on Cell Proliferation and Migration

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