Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid

Nagehan Saltan1, H Mehtap Kutlu2, Deniz H&uuml;r3,4, Arzu İşcan4, Ridvan Say31Department of Pharmaceutical Botanics, Faculty of Pharmacy, 2Department of Biology, 3Department of Chemistry, Faculty of Science, 4Plant, Drug and Scientific Research Center Anadolu University, Eskişehir, TurkeyBac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nagehan Saltan, H Mehtap Kutlu, Deniz H&uuml;r
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4152f82ddf30417d9e0509d0ebfb0d9f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4152f82ddf30417d9e0509d0ebfb0d9f
record_format	dspace
spelling	oai:doaj.org-article:4152f82ddf30417d9e0509d0ebfb0d9f2021-12-02T05:39:40ZInteraction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid1176-91141178-2013https://doaj.org/article/4152f82ddf30417d9e0509d0ebfb0d9f2011-02-01T00:00:00Zhttp://www.dovepress.com/interaction-of-cancer-cells-with-magnetic-nanoparticles-modified-by-me-a6489https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Nagehan Saltan1, H Mehtap Kutlu2, Deniz H&uuml;r3,4, Arzu İşcan4, Ridvan Say31Department of Pharmaceutical Botanics, Faculty of Pharmacy, 2Department of Biology, 3Department of Chemistry, Faculty of Science, 4Plant, Drug and Scientific Research Center Anadolu University, Eskişehir, TurkeyBackground: Magnetic nanoparticles show great promise for use as tools in a wide variety of biomedical applications. The purpose of this study was to investigate the potential effects of methacrylamido-folic acid (Ma-Fol)-modified magnetic nanoparticles on 5RP7 (H-ras-transformed rat embryonic fibroblasts) and NIH/3T3 (normal mouse embryonic fibroblasts).Methods: The cytotoxicity and viability of 5RP7 and NIH/3T3 cells were detected. The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Nanoparticle internalization into 5RP7 and NIH/3T3 cells was visualized by transmission electron microscopy.Conclusion: In this study, folic acid coupled to the surface of iron oxide for selective binding to cancer cells and immobilized the surfaces of magnetic nanoparticles. This complex improves cell internalization and targeting of cancer cells. We detected increased apoptosis using flow cytometry and transmission electron microscopy.Results: Folic acid modification of magnetic nanoparticles could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of folic-acid modified nanoparticles by 5RP7 cancer cells was also much higher than that of 3T3 cells. This modification can be used for successful targeting of cancer cells expressing the folate receptor.Keywords: folic acid, apoptosis, nanoparticles, transmission electron microscopy Nagehan SaltanH Mehtap KutluDeniz H&uuml;rDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 477-484 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nagehan Saltan H Mehtap Kutlu Deniz H&uuml;r Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
description	Nagehan Saltan1, H Mehtap Kutlu2, Deniz H&uuml;r3,4, Arzu İşcan4, Ridvan Say31Department of Pharmaceutical Botanics, Faculty of Pharmacy, 2Department of Biology, 3Department of Chemistry, Faculty of Science, 4Plant, Drug and Scientific Research Center Anadolu University, Eskişehir, TurkeyBackground: Magnetic nanoparticles show great promise for use as tools in a wide variety of biomedical applications. The purpose of this study was to investigate the potential effects of methacrylamido-folic acid (Ma-Fol)-modified magnetic nanoparticles on 5RP7 (H-ras-transformed rat embryonic fibroblasts) and NIH/3T3 (normal mouse embryonic fibroblasts).Methods: The cytotoxicity and viability of 5RP7 and NIH/3T3 cells were detected. The percentage of cells undergoing apoptosis was analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Nanoparticle internalization into 5RP7 and NIH/3T3 cells was visualized by transmission electron microscopy.Conclusion: In this study, folic acid coupled to the surface of iron oxide for selective binding to cancer cells and immobilized the surfaces of magnetic nanoparticles. This complex improves cell internalization and targeting of cancer cells. We detected increased apoptosis using flow cytometry and transmission electron microscopy.Results: Folic acid modification of magnetic nanoparticles could be used to facilitate uptake to specific cancer cells for cancer therapy and diagnosis. Our results showed that the uptake of folic-acid modified nanoparticles by 5RP7 cancer cells was also much higher than that of 3T3 cells. This modification can be used for successful targeting of cancer cells expressing the folate receptor.Keywords: folic acid, apoptosis, nanoparticles, transmission electron microscopy
format	article
author	Nagehan Saltan H Mehtap Kutlu Deniz H&uuml;r
author_facet	Nagehan Saltan H Mehtap Kutlu Deniz H&uuml;r
author_sort	Nagehan Saltan
title	Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
title_short	Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
title_full	Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
title_fullStr	Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
title_full_unstemmed	Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
title_sort	interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/4152f82ddf30417d9e0509d0ebfb0d9f
work_keys_str_mv	AT nagehansaltan interactionofcancercellswithmagneticnanoparticlesmodifiedbymethacrylamidofolicacid AT hmehtapkutlu interactionofcancercellswithmagneticnanoparticlesmodifiedbymethacrylamidofolicacid AT denizhampuumlr interactionofcancercellswithmagneticnanoparticlesmodifiedbymethacrylamidofolicacid
_version_	1718400292111777792

Interaction of cancer cells with magnetic nanoparticles modified by methacrylamido-folic acid

Ejemplares similares