Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis

Abstract Biomass production requires the coordination between growth and metabolism. In a large-scale screen for mutants affected in leaf morphology, we isolated the orbiculata1 (orb1) mutants, which exhibit a pale green phenotype and reduced growth. The combination of map-based cloning and next-gen...

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Autores principales:	Tamara Muñoz-Nortes, José Manuel Pérez-Pérez, Raquel Sarmiento-Mañús, Héctor Candela, José Luis Micol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/415bba402abc4ec39f35b5ee7e9aeb4b
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spelling	oai:doaj.org-article:415bba402abc4ec39f35b5ee7e9aeb4b2021-12-02T11:41:21ZDeficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis10.1038/s41598-017-06335-42045-2322https://doaj.org/article/415bba402abc4ec39f35b5ee7e9aeb4b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06335-4https://doaj.org/toc/2045-2322Abstract Biomass production requires the coordination between growth and metabolism. In a large-scale screen for mutants affected in leaf morphology, we isolated the orbiculata1 (orb1) mutants, which exhibit a pale green phenotype and reduced growth. The combination of map-based cloning and next-generation sequencing allowed us to establish that ORB1 encodes the GLUTAMATE SYNTHASE 1 (GLU1) enzyme, also known as FERREDOXIN-DEPENDENT GLUTAMINE OXOGLUTARATE AMINOTRANSFERASE 1 (Fd-GOGAT1). We performed an RNA-seq analysis to identify global gene expression changes in the orb1–3 mutant. We found altered expression levels of genes encoding enzymes involved in nitrogen assimilation and amino acid biosynthesis, such as glutamine synthetases, asparagine synthetases and glutamate dehydrogenases, showing that the expression of these genes depends on the levels of glutamine and/or glutamate. In addition, we observed a concerted upregulation of genes encoding subunits of the cytosolic ribosome. A gene ontology (GO) analysis of the differentially expressed genes between Ler and orb1–3 showed that the most enriched GO terms were ‘translation’, ‘cytosolic ribosome’ and ‘structural constituent of ribosome’. The upregulation of ribosome-related functions might reflect an attempt to keep protein synthesis at optimal levels even when the pool of glutamate is reduced.Tamara Muñoz-NortesJosé Manuel Pérez-PérezRaquel Sarmiento-MañúsHéctor CandelaJosé Luis MicolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tamara Muñoz-Nortes José Manuel Pérez-Pérez Raquel Sarmiento-Mañús Héctor Candela José Luis Micol Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
description	Abstract Biomass production requires the coordination between growth and metabolism. In a large-scale screen for mutants affected in leaf morphology, we isolated the orbiculata1 (orb1) mutants, which exhibit a pale green phenotype and reduced growth. The combination of map-based cloning and next-generation sequencing allowed us to establish that ORB1 encodes the GLUTAMATE SYNTHASE 1 (GLU1) enzyme, also known as FERREDOXIN-DEPENDENT GLUTAMINE OXOGLUTARATE AMINOTRANSFERASE 1 (Fd-GOGAT1). We performed an RNA-seq analysis to identify global gene expression changes in the orb1–3 mutant. We found altered expression levels of genes encoding enzymes involved in nitrogen assimilation and amino acid biosynthesis, such as glutamine synthetases, asparagine synthetases and glutamate dehydrogenases, showing that the expression of these genes depends on the levels of glutamine and/or glutamate. In addition, we observed a concerted upregulation of genes encoding subunits of the cytosolic ribosome. A gene ontology (GO) analysis of the differentially expressed genes between Ler and orb1–3 showed that the most enriched GO terms were ‘translation’, ‘cytosolic ribosome’ and ‘structural constituent of ribosome’. The upregulation of ribosome-related functions might reflect an attempt to keep protein synthesis at optimal levels even when the pool of glutamate is reduced.
format	article
author	Tamara Muñoz-Nortes José Manuel Pérez-Pérez Raquel Sarmiento-Mañús Héctor Candela José Luis Micol
author_facet	Tamara Muñoz-Nortes José Manuel Pérez-Pérez Raquel Sarmiento-Mañús Héctor Candela José Luis Micol
author_sort	Tamara Muñoz-Nortes
title	Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
title_short	Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
title_full	Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
title_fullStr	Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
title_full_unstemmed	Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis
title_sort	deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in arabidopsis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/415bba402abc4ec39f35b5ee7e9aeb4b
work_keys_str_mv	AT tamaramunoznortes deficientglutamatebiosynthesistriggersaconcertedupregulationofribosomalproteingenesinarabidopsis AT josemanuelperezperez deficientglutamatebiosynthesistriggersaconcertedupregulationofribosomalproteingenesinarabidopsis AT raquelsarmientomanus deficientglutamatebiosynthesistriggersaconcertedupregulationofribosomalproteingenesinarabidopsis AT hectorcandela deficientglutamatebiosynthesistriggersaconcertedupregulationofribosomalproteingenesinarabidopsis AT joseluismicol deficientglutamatebiosynthesistriggersaconcertedupregulationofribosomalproteingenesinarabidopsis
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Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis

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