An integrated microfluidic device for the sorting of yeast cells using image processing

Abstract The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usu...

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Autores principales:	Bo Yang Yu, Caglar Elbuken, Chong Shen, Jan Paul Huissoon, Carolyn L. Ren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/415de692be644001b249ce7326169f5c
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spelling	oai:doaj.org-article:415de692be644001b249ce7326169f5c2021-12-02T15:08:53ZAn integrated microfluidic device for the sorting of yeast cells using image processing10.1038/s41598-018-21833-92045-2322https://doaj.org/article/415de692be644001b249ce7326169f5c2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21833-9https://doaj.org/toc/2045-2322Abstract The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.Bo Yang YuCaglar ElbukenChong ShenJan Paul HuissoonCarolyn L. RenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bo Yang Yu Caglar Elbuken Chong Shen Jan Paul Huissoon Carolyn L. Ren An integrated microfluidic device for the sorting of yeast cells using image processing
description	Abstract The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.
format	article
author	Bo Yang Yu Caglar Elbuken Chong Shen Jan Paul Huissoon Carolyn L. Ren
author_facet	Bo Yang Yu Caglar Elbuken Chong Shen Jan Paul Huissoon Carolyn L. Ren
author_sort	Bo Yang Yu
title	An integrated microfluidic device for the sorting of yeast cells using image processing
title_short	An integrated microfluidic device for the sorting of yeast cells using image processing
title_full	An integrated microfluidic device for the sorting of yeast cells using image processing
title_fullStr	An integrated microfluidic device for the sorting of yeast cells using image processing
title_full_unstemmed	An integrated microfluidic device for the sorting of yeast cells using image processing
title_sort	integrated microfluidic device for the sorting of yeast cells using image processing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/415de692be644001b249ce7326169f5c
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An integrated microfluidic device for the sorting of yeast cells using image processing

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