Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis

Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysaccharide (LPS) and i...

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Autores principales:	Ana Paula Girol, Caroline de Freitas Zanon, Ícaro Putinhon Caruso, Sara de Souza Costa, Helena Ribeiro Souza, Marinônio Lopes Cornélio, Sonia Maria Oliani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	eye inflammation lipopolysaccharide natural bioactive extracts Ac2-26 FPR receptor inflammatory mediators Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4177ebd7987e4a73becc2b9f4ec34c17
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spelling	oai:doaj.org-article:4177ebd7987e4a73becc2b9f4ec34c172021-11-25T17:12:18ZAnnexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis10.3390/cells101131702073-4409https://doaj.org/article/4177ebd7987e4a73becc2b9f4ec34c172021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3170https://doaj.org/toc/2073-4409Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysaccharide (LPS) and intraperitoneally treated with Ac2-26 (200 µg), PL (200 and 400 µg), or Ac2-26 + PL after 15 min. Then, 24 h after LPS inoculation, leukocytes in aqueous humor, mononuclear cells, AnxA1, formyl peptide receptor (fpr)1, fpr2, and cyclooxygenase (COX)-2 were evaluated in the ocular tissues, along with inflammatory mediators in the blood and macerated supernatant. Decreased leukocyte influx, levels of inflammatory mediators, and COX-2 expression confirmed the anti-inflammatory actions of the peptide and pointed to the protective effects of PL at higher dosage. However, when PL and Ac2-26 were administered in combination, the inflammatory potential was lost. AnxA1 expression was elevated among groups treated with PL or Ac2-26 + PL but reduced after treatment with Ac2-26. Fpr2 expression was increased only in untreated EIU and Ac2-26 groups. The interaction between Ac2-26 and PL negatively affected the anti-inflammatory action of Ac2-26 or PL. We emphasize that the anti-inflammatory effects of PL can be used as a therapeutic strategy to protect against uveitis.Ana Paula GirolCaroline de Freitas ZanonÍcaro Putinhon CarusoSara de Souza CostaHelena Ribeiro SouzaMarinônio Lopes CornélioSonia Maria OlianiMDPI AGarticleeye inflammationlipopolysaccharidenatural bioactive extractsAc2-26FPR receptorinflammatory mediatorsBiology (General)QH301-705.5ENCells, Vol 10, Iss 3170, p 3170 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eye inflammation lipopolysaccharide natural bioactive extracts Ac2-26 FPR receptor inflammatory mediators Biology (General) QH301-705.5
spellingShingle	eye inflammation lipopolysaccharide natural bioactive extracts Ac2-26 FPR receptor inflammatory mediators Biology (General) QH301-705.5 Ana Paula Girol Caroline de Freitas Zanon Ícaro Putinhon Caruso Sara de Souza Costa Helena Ribeiro Souza Marinônio Lopes Cornélio Sonia Maria Oliani Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
description	Uveitis is one of the main causes of blindness worldwide, and therapeutic alternatives are worthy of study. We investigated the effects of piperlongumine (PL) and/or annexin A1 (AnxA1) mimetic peptide Ac2-26 on endotoxin-induced uveitis (EIU). Rats were inoculated with lipopolysaccharide (LPS) and intraperitoneally treated with Ac2-26 (200 µg), PL (200 and 400 µg), or Ac2-26 + PL after 15 min. Then, 24 h after LPS inoculation, leukocytes in aqueous humor, mononuclear cells, AnxA1, formyl peptide receptor (fpr)1, fpr2, and cyclooxygenase (COX)-2 were evaluated in the ocular tissues, along with inflammatory mediators in the blood and macerated supernatant. Decreased leukocyte influx, levels of inflammatory mediators, and COX-2 expression confirmed the anti-inflammatory actions of the peptide and pointed to the protective effects of PL at higher dosage. However, when PL and Ac2-26 were administered in combination, the inflammatory potential was lost. AnxA1 expression was elevated among groups treated with PL or Ac2-26 + PL but reduced after treatment with Ac2-26. Fpr2 expression was increased only in untreated EIU and Ac2-26 groups. The interaction between Ac2-26 and PL negatively affected the anti-inflammatory action of Ac2-26 or PL. We emphasize that the anti-inflammatory effects of PL can be used as a therapeutic strategy to protect against uveitis.
format	article
author	Ana Paula Girol Caroline de Freitas Zanon Ícaro Putinhon Caruso Sara de Souza Costa Helena Ribeiro Souza Marinônio Lopes Cornélio Sonia Maria Oliani
author_facet	Ana Paula Girol Caroline de Freitas Zanon Ícaro Putinhon Caruso Sara de Souza Costa Helena Ribeiro Souza Marinônio Lopes Cornélio Sonia Maria Oliani
author_sort	Ana Paula Girol
title	Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
title_short	Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
title_full	Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
title_fullStr	Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
title_full_unstemmed	Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis
title_sort	annexin a1 mimetic peptide and piperlongumine: anti-inflammatory profiles in endotoxin-induced uveitis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4177ebd7987e4a73becc2b9f4ec34c17
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Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis

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