Neuronal imaging with ultrahigh dynamic range multiphoton microscopy

Abstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves...

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Auteurs principaux:	Ruohui Yang, Timothy D. Weber, Ellen D. Witkowski, Ian G. Davison, Jerome Mertz
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2017
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/418a4726ec0748a2a28bf1864c3ab081
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spelling	oai:doaj.org-article:418a4726ec0748a2a28bf1864c3ab0812021-12-02T15:06:15ZNeuronal imaging with ultrahigh dynamic range multiphoton microscopy10.1038/s41598-017-06065-72045-2322https://doaj.org/article/418a4726ec0748a2a28bf1864c3ab0812017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06065-7https://doaj.org/toc/2045-2322Abstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation of the sample strength to accommodate ultrahigh dynamic range without loss of information. No microscope hardware modifications are required, making the technique readily compatible with commercial instruments. Benefits are shown in both structural and in-vivo functional mouse brain imaging applications.Ruohui YangTimothy D. WeberEllen D. WitkowskiIan G. DavisonJerome MertzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
description	Abstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation of the sample strength to accommodate ultrahigh dynamic range without loss of information. No microscope hardware modifications are required, making the technique readily compatible with commercial instruments. Benefits are shown in both structural and in-vivo functional mouse brain imaging applications.
format	article
author	Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz
author_facet	Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz
author_sort	Ruohui Yang
title	Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
title_short	Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
title_full	Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
title_fullStr	Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
title_full_unstemmed	Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
title_sort	neuronal imaging with ultrahigh dynamic range multiphoton microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/418a4726ec0748a2a28bf1864c3ab081
work_keys_str_mv	AT ruohuiyang neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT timothydweber neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT ellendwitkowski neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT iangdavison neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT jeromemertz neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy
_version_	1718388507378974720

Neuronal imaging with ultrahigh dynamic range multiphoton microscopy

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