Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers

Surniyantoro HNE, Lusiyanti Y, Rahardjo T, Nurhayati S, Tetriana D. 2018. Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers. Biodiversitas 19: 1676-1682. This study was aimed to examine the association between XRCC1...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	HARRY NUGROHO EKO SURNIYANTORO, YANTI LUSIYANTI, TUR RAHARDJO, SITI NURHAYATI, DEVITA TETRIANA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	gene polymorphism xrcc1 exon 10 micronucleus ionizing radiation radiation workers Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/418d97c40892423fadcbdca73e49d362
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:418d97c40892423fadcbdca73e49d362
record_format	dspace
spelling	oai:doaj.org-article:418d97c40892423fadcbdca73e49d3622021-11-16T14:04:05ZAssociation between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers1412-033X2085-472210.13057/biodiv/d190512https://doaj.org/article/418d97c40892423fadcbdca73e49d3622018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2929https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Surniyantoro HNE, Lusiyanti Y, Rahardjo T, Nurhayati S, Tetriana D. 2018. Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers. Biodiversitas 19: 1676-1682. This study was aimed to examine the association between XRCC1 exon 10 gene polymorphism and micronucleus frequencies in radiation workers and their relation to the confounding factors. This study involved 37 radiation workers and 37 controls from several hospitals in Indonesia. Genotyping of X-ray cross-complementing group 1 (XRCC1) exon 10 gene polymorphism and micronucleus assay were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Cytokinesis-Block Micronucleus assay (CBMN assay), respectively. The results indicated that MN frequencies were not significantly higher in the exposed workers than in controls (20.46±6.42 versus 16.89 ±9.72; P=0.07). The micronucleus frequencies of radiation workers with mutant genotype showed not significantly higher than controls in the same genotypes (22±6.64 versus 11.75 ± 8.13; P=0.11). The confounding factors, like age, years of employment and equivalent doses were significantly associated with micronucleus frequencies (P<0.05). The equivalent dose has a significantly positive correlation with micronucleus frequencies among radiation workers, increasing the MN frequencies by 16.3 per 1 mSv of equivalent dose (P=0.001). The genetic polymorphism of XRCC1 gene exon 10 demonstrated no association with the extent of DNA damage in the hospital radiation workers. The MN frequencies were strongly associated with age, equivalent dose and years of employment.HARRY NUGROHO EKO SURNIYANTOROYANTI LUSIYANTITUR RAHARDJOSITI NURHAYATIDEVITA TETRIANAMBI & UNS Soloarticlegene polymorphismxrcc1 exon 10micronucleusionizing radiationradiation workersBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1676-1682 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene polymorphism xrcc1 exon 10 micronucleus ionizing radiation radiation workers Biology (General) QH301-705.5
spellingShingle	gene polymorphism xrcc1 exon 10 micronucleus ionizing radiation radiation workers Biology (General) QH301-705.5 HARRY NUGROHO EKO SURNIYANTORO YANTI LUSIYANTI TUR RAHARDJO SITI NURHAYATI DEVITA TETRIANA Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
description	Surniyantoro HNE, Lusiyanti Y, Rahardjo T, Nurhayati S, Tetriana D. 2018. Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers. Biodiversitas 19: 1676-1682. This study was aimed to examine the association between XRCC1 exon 10 gene polymorphism and micronucleus frequencies in radiation workers and their relation to the confounding factors. This study involved 37 radiation workers and 37 controls from several hospitals in Indonesia. Genotyping of X-ray cross-complementing group 1 (XRCC1) exon 10 gene polymorphism and micronucleus assay were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Cytokinesis-Block Micronucleus assay (CBMN assay), respectively. The results indicated that MN frequencies were not significantly higher in the exposed workers than in controls (20.46±6.42 versus 16.89 ±9.72; P=0.07). The micronucleus frequencies of radiation workers with mutant genotype showed not significantly higher than controls in the same genotypes (22±6.64 versus 11.75 ± 8.13; P=0.11). The confounding factors, like age, years of employment and equivalent doses were significantly associated with micronucleus frequencies (P<0.05). The equivalent dose has a significantly positive correlation with micronucleus frequencies among radiation workers, increasing the MN frequencies by 16.3 per 1 mSv of equivalent dose (P=0.001). The genetic polymorphism of XRCC1 gene exon 10 demonstrated no association with the extent of DNA damage in the hospital radiation workers. The MN frequencies were strongly associated with age, equivalent dose and years of employment.
format	article
author	HARRY NUGROHO EKO SURNIYANTORO YANTI LUSIYANTI TUR RAHARDJO SITI NURHAYATI DEVITA TETRIANA
author_facet	HARRY NUGROHO EKO SURNIYANTORO YANTI LUSIYANTI TUR RAHARDJO SITI NURHAYATI DEVITA TETRIANA
author_sort	HARRY NUGROHO EKO SURNIYANTORO
title	Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
title_short	Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
title_full	Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
title_fullStr	Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
title_full_unstemmed	Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers
title_sort	association between xrcc1 exon 10 (arg399gln) gene polymorphism and micronucleus as a predictor of dna damage among radiation workers
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/418d97c40892423fadcbdca73e49d362
work_keys_str_mv	AT harrynugrohoekosurniyantoro associationbetweenxrcc1exon10arg399glngenepolymorphismandmicronucleusasapredictorofdnadamageamongradiationworkers AT yantilusiyanti associationbetweenxrcc1exon10arg399glngenepolymorphismandmicronucleusasapredictorofdnadamageamongradiationworkers AT turrahardjo associationbetweenxrcc1exon10arg399glngenepolymorphismandmicronucleusasapredictorofdnadamageamongradiationworkers AT sitinurhayati associationbetweenxrcc1exon10arg399glngenepolymorphismandmicronucleusasapredictorofdnadamageamongradiationworkers AT devitatetriana associationbetweenxrcc1exon10arg399glngenepolymorphismandmicronucleusasapredictorofdnadamageamongradiationworkers
_version_	1718426415707193344

Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers

Ejemplares similares