Gene expression profiles characterize inflammation stages in the acute lung injury in mice.

Acute Lung Injury (ALI) carries about 50 percent mortality and is frequently associated with an infection (sepsis). Life-support treatment with mechanical ventilation rescues many patients, although superimposed infection or multiple organ failure can result in death. The outcome of a patient develo...

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Autores principales:	Isabelle Lesur, Julien Textoris, Béatrice Loriod, Cécile Courbon, Stéphane Garcia, Marc Leone, Catherine Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/419340c2133145949d9e92671a6e5036
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spelling	oai:doaj.org-article:419340c2133145949d9e92671a6e50362021-12-02T20:20:14ZGene expression profiles characterize inflammation stages in the acute lung injury in mice.1932-620310.1371/journal.pone.0011485https://doaj.org/article/419340c2133145949d9e92671a6e50362010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20628605/?tool=EBIhttps://doaj.org/toc/1932-6203Acute Lung Injury (ALI) carries about 50 percent mortality and is frequently associated with an infection (sepsis). Life-support treatment with mechanical ventilation rescues many patients, although superimposed infection or multiple organ failure can result in death. The outcome of a patient developing sepsis depends on two factors: the infection and the pre-existing inflammation. In this study, we described each stage of the inflammation process using a transcriptional approach and an animal model. Female C57BL6/J mice received an intravenous oleic acid injection to induce an acute lung injury (ALI). Lung expression patterns were analyzed using a 9900 cDNA mouse microarray (MUSV29K). Our gene-expression analysis revealed marked changes in the immune and inflammatory response metabolic pathways, notably lipid metabolism and transcription. The early stage (1 hour-1.5 hours) is characterized by a pro-inflammatory immune response. Later (3 hours-4 hours), the immune cells migrate into inflamed tissues through interaction with vascular endothelial cells. Finally, at late stages of lung inflammation (18 hours-24 hours), metabolism is deeply disturbed. Highly expressed pro-inflammatory cytokines activate transcription of many genes and lipid metabolism. In this study, we described a global overview of critical events occurring during lung inflammation which is essential to understand infectious pathologies such as sepsis where inflammation and infection are intertwined. Based on these data, it becomes possible to isolate the impact of a pathogen at the transcriptional level from the global gene expression modifications resulting from the infection associated with the inflammation.Isabelle LesurJulien TextorisBéatrice LoriodCécile CourbonStéphane GarciaMarc LeoneCatherine NguyenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11485 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isabelle Lesur Julien Textoris Béatrice Loriod Cécile Courbon Stéphane Garcia Marc Leone Catherine Nguyen Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
description	Acute Lung Injury (ALI) carries about 50 percent mortality and is frequently associated with an infection (sepsis). Life-support treatment with mechanical ventilation rescues many patients, although superimposed infection or multiple organ failure can result in death. The outcome of a patient developing sepsis depends on two factors: the infection and the pre-existing inflammation. In this study, we described each stage of the inflammation process using a transcriptional approach and an animal model. Female C57BL6/J mice received an intravenous oleic acid injection to induce an acute lung injury (ALI). Lung expression patterns were analyzed using a 9900 cDNA mouse microarray (MUSV29K). Our gene-expression analysis revealed marked changes in the immune and inflammatory response metabolic pathways, notably lipid metabolism and transcription. The early stage (1 hour-1.5 hours) is characterized by a pro-inflammatory immune response. Later (3 hours-4 hours), the immune cells migrate into inflamed tissues through interaction with vascular endothelial cells. Finally, at late stages of lung inflammation (18 hours-24 hours), metabolism is deeply disturbed. Highly expressed pro-inflammatory cytokines activate transcription of many genes and lipid metabolism. In this study, we described a global overview of critical events occurring during lung inflammation which is essential to understand infectious pathologies such as sepsis where inflammation and infection are intertwined. Based on these data, it becomes possible to isolate the impact of a pathogen at the transcriptional level from the global gene expression modifications resulting from the infection associated with the inflammation.
format	article
author	Isabelle Lesur Julien Textoris Béatrice Loriod Cécile Courbon Stéphane Garcia Marc Leone Catherine Nguyen
author_facet	Isabelle Lesur Julien Textoris Béatrice Loriod Cécile Courbon Stéphane Garcia Marc Leone Catherine Nguyen
author_sort	Isabelle Lesur
title	Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
title_short	Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
title_full	Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
title_fullStr	Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
title_full_unstemmed	Gene expression profiles characterize inflammation stages in the acute lung injury in mice.
title_sort	gene expression profiles characterize inflammation stages in the acute lung injury in mice.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/419340c2133145949d9e92671a6e5036
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Gene expression profiles characterize inflammation stages in the acute lung injury in mice.

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