Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model

Despite antigen affinity of B cells varying from cell to cell, functional analyses of antigen-reactive B cells on individual B cells are missing due to technical difficulties. Especially in the field of autoimmune diseases, promising pathogenic B cells have not been adequately studied to date becaus...

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Autores principales:	Takemichi Fukasawa, Ayumi Yoshizaki, Satoshi Ebata, Asako Yoshizaki-Ogawa, Yoshihide Asano, Atsushi Enomoto, Kiyoshi Miyagawa, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori, Shinichi Sato
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	B cell systemic sclerosis fibrosis single cell analysis cytokine Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/41969bc1d4ab4eb0b5535a40e0a4f551
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spelling	oai:doaj.org-article:41969bc1d4ab4eb0b5535a40e0a4f5512021-12-02T10:23:44ZSingle-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model10.7554/eLife.672092050-084Xe67209https://doaj.org/article/41969bc1d4ab4eb0b5535a40e0a4f5512021-12-01T00:00:00Zhttps://elifesciences.org/articles/67209https://doaj.org/toc/2050-084XDespite antigen affinity of B cells varying from cell to cell, functional analyses of antigen-reactive B cells on individual B cells are missing due to technical difficulties. Especially in the field of autoimmune diseases, promising pathogenic B cells have not been adequately studied to date because of its rarity. In this study, functions of autoantigen-reactive B cells in autoimmune disease were analyzed at the single-cell level. Since topoisomerase I is a distinct autoantigen, we targeted systemic sclerosis as autoimmune disease. Decreased and increased affinities for topoisomerase I of topoisomerase I-reactive B cells led to anti-inflammatory and pro-inflammatory cytokine production associated with the inhibition and development of fibrosis, which is the major symptom of systemic sclerosis. Furthermore, inhibition of pro-inflammatory cytokine production and increased affinity of topoisomerase I-reactive B cells suppressed fibrosis. These results indicate that autoantigen-reactive B cells contribute to the disease manifestations in autoimmune disease through their antigen affinity.Takemichi FukasawaAyumi YoshizakiSatoshi EbataAsako Yoshizaki-OgawaYoshihide AsanoAtsushi EnomotoKiyoshi MiyagawaYutaka KazoeKazuma MawatariTakehiko KitamoriShinichi SatoeLife Sciences Publications LtdarticleB cellsystemic sclerosisfibrosissingle cell analysiscytokineMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	B cell systemic sclerosis fibrosis single cell analysis cytokine Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	B cell systemic sclerosis fibrosis single cell analysis cytokine Medicine R Science Q Biology (General) QH301-705.5 Takemichi Fukasawa Ayumi Yoshizaki Satoshi Ebata Asako Yoshizaki-Ogawa Yoshihide Asano Atsushi Enomoto Kiyoshi Miyagawa Yutaka Kazoe Kazuma Mawatari Takehiko Kitamori Shinichi Sato Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
description	Despite antigen affinity of B cells varying from cell to cell, functional analyses of antigen-reactive B cells on individual B cells are missing due to technical difficulties. Especially in the field of autoimmune diseases, promising pathogenic B cells have not been adequately studied to date because of its rarity. In this study, functions of autoantigen-reactive B cells in autoimmune disease were analyzed at the single-cell level. Since topoisomerase I is a distinct autoantigen, we targeted systemic sclerosis as autoimmune disease. Decreased and increased affinities for topoisomerase I of topoisomerase I-reactive B cells led to anti-inflammatory and pro-inflammatory cytokine production associated with the inhibition and development of fibrosis, which is the major symptom of systemic sclerosis. Furthermore, inhibition of pro-inflammatory cytokine production and increased affinity of topoisomerase I-reactive B cells suppressed fibrosis. These results indicate that autoantigen-reactive B cells contribute to the disease manifestations in autoimmune disease through their antigen affinity.
format	article
author	Takemichi Fukasawa Ayumi Yoshizaki Satoshi Ebata Asako Yoshizaki-Ogawa Yoshihide Asano Atsushi Enomoto Kiyoshi Miyagawa Yutaka Kazoe Kazuma Mawatari Takehiko Kitamori Shinichi Sato
author_facet	Takemichi Fukasawa Ayumi Yoshizaki Satoshi Ebata Asako Yoshizaki-Ogawa Yoshihide Asano Atsushi Enomoto Kiyoshi Miyagawa Yutaka Kazoe Kazuma Mawatari Takehiko Kitamori Shinichi Sato
author_sort	Takemichi Fukasawa
title	Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
title_short	Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
title_full	Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
title_fullStr	Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
title_full_unstemmed	Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
title_sort	single-cell-level protein analysis revealing the roles of autoantigen-reactive b lymphocytes in autoimmune disease and the murine model
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/41969bc1d4ab4eb0b5535a40e0a4f551
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Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model

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