Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging

Abstract Cerenkov luminescence imaging (CLI) has been an evolutional and alternative approach of nuclear imaging in basic research. This study aimed to measure the 131I thyroid uptake of mouse using CLI for assessment of thyroid function. Quantification of 131I thyroid uptake of mice in euthyroid, h...

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Autores principales:	Chien-Chih Ke, Zi-Ming He, Ya-Ju Hsieh, Chia-Wen Huang, Jia-Je Li, Luen Hwu, Yi-An Chen, Bang-Hung Yang, Chi-Wei Chang, Wen-Sheng Huang, Ren-Shyan Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41993a48aaec4a2da9202f5bf8e194ef
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spelling	oai:doaj.org-article:41993a48aaec4a2da9202f5bf8e194ef2021-12-02T15:05:47ZQuantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging10.1038/s41598-017-05516-52045-2322https://doaj.org/article/41993a48aaec4a2da9202f5bf8e194ef2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05516-5https://doaj.org/toc/2045-2322Abstract Cerenkov luminescence imaging (CLI) has been an evolutional and alternative approach of nuclear imaging in basic research. This study aimed to measure the 131I thyroid uptake of mouse using CLI for assessment of thyroid function. Quantification of 131I thyroid uptake of mice in euthyroid, hypothyroid and hyperthyroid status was performed by CLI and γ-scintigraphy at 24 hours after injection of 131I. The 131I thyroid uptake was calculated using the equation: (thyroid counts − background counts)/(counts of injected dose of 131I) × 100%. Serum T4 concentration was determined to evaluate the thyroid function. The radioactivity of 131I was linearly correlated with the CL signals in both in vitro and in vivo measurements. CLI showed a significant decrease and increase of 131I thyroid uptake in the mice in hypo- and hyperfunctioning status, respectively, and highly correlated with that measured by γ-scintigraphy. However, the percent thyroid uptake measured by CLI were one-fifth of those measured by γ-scintigraphy due to insufficient tissue penetration of CL. These results indicate that CLI, in addition to nuclear imaging, is able to image and evaluate the 131I thyroid uptake function in mice in preclinical and research settings.Chien-Chih KeZi-Ming HeYa-Ju HsiehChia-Wen HuangJia-Je LiLuen HwuYi-An ChenBang-Hung YangChi-Wei ChangWen-Sheng HuangRen-Shyan LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chien-Chih Ke Zi-Ming He Ya-Ju Hsieh Chia-Wen Huang Jia-Je Li Luen Hwu Yi-An Chen Bang-Hung Yang Chi-Wei Chang Wen-Sheng Huang Ren-Shyan Liu Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
description	Abstract Cerenkov luminescence imaging (CLI) has been an evolutional and alternative approach of nuclear imaging in basic research. This study aimed to measure the 131I thyroid uptake of mouse using CLI for assessment of thyroid function. Quantification of 131I thyroid uptake of mice in euthyroid, hypothyroid and hyperthyroid status was performed by CLI and γ-scintigraphy at 24 hours after injection of 131I. The 131I thyroid uptake was calculated using the equation: (thyroid counts − background counts)/(counts of injected dose of 131I) × 100%. Serum T4 concentration was determined to evaluate the thyroid function. The radioactivity of 131I was linearly correlated with the CL signals in both in vitro and in vivo measurements. CLI showed a significant decrease and increase of 131I thyroid uptake in the mice in hypo- and hyperfunctioning status, respectively, and highly correlated with that measured by γ-scintigraphy. However, the percent thyroid uptake measured by CLI were one-fifth of those measured by γ-scintigraphy due to insufficient tissue penetration of CL. These results indicate that CLI, in addition to nuclear imaging, is able to image and evaluate the 131I thyroid uptake function in mice in preclinical and research settings.
format	article
author	Chien-Chih Ke Zi-Ming He Ya-Ju Hsieh Chia-Wen Huang Jia-Je Li Luen Hwu Yi-An Chen Bang-Hung Yang Chi-Wei Chang Wen-Sheng Huang Ren-Shyan Liu
author_facet	Chien-Chih Ke Zi-Ming He Ya-Ju Hsieh Chia-Wen Huang Jia-Je Li Luen Hwu Yi-An Chen Bang-Hung Yang Chi-Wei Chang Wen-Sheng Huang Ren-Shyan Liu
author_sort	Chien-Chih Ke
title	Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
title_short	Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
title_full	Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
title_fullStr	Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
title_full_unstemmed	Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging
title_sort	quantitative measurement of the thyroid uptake function of mouse by cerenkov luminescence imaging
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/41993a48aaec4a2da9202f5bf8e194ef
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Quantitative Measurement of the Thyroid Uptake Function of Mouse by Cerenkov Luminescence Imaging

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