Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy

Abstract Nanodiamonds (NDs) have attracted considerable attention as drug delivery nanocarriers due to their low cytotoxicity and facile surface functionalization. Given these features, NDs have been recently investigated for the fabrication of nanocomposite hydrogels for tissue engineering. Here we...

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Autores principales:	Settimio Pacelli, Ryan Maloney, Aparna R. Chakravarti, Jonathan Whitlow, Sayantani Basu, Saman Modaresi, Stevin Gehrke, Arghya Paul
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/419b8269cf4944669490cb1ab703329e
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spelling	oai:doaj.org-article:419b8269cf4944669490cb1ab703329e2021-12-02T15:06:02ZControlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy10.1038/s41598-017-06028-y2045-2322https://doaj.org/article/419b8269cf4944669490cb1ab703329e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06028-yhttps://doaj.org/toc/2045-2322Abstract Nanodiamonds (NDs) have attracted considerable attention as drug delivery nanocarriers due to their low cytotoxicity and facile surface functionalization. Given these features, NDs have been recently investigated for the fabrication of nanocomposite hydrogels for tissue engineering. Here we report the synthesis of a hydrogel using photocrosslinkable gelatin methacrylamide (GelMA) and NDs as a three-dimensional scaffold for drug delivery and stem cell-guided bone regeneration. We investigated the effect of different concentration of NDs on the physical and mechanical properties of the GelMA hydrogel network. The inclusion of NDs increased the network stiffness, which in turn augmented the traction forces generated by human adipose stem cells (hASCs). We also tested the ability of NDs to adsorb and modulate the release of a model drug dexamethasone (Dex) to promote the osteogenic differentiation of hASCs. The ND-Dex complexes modulated gene expression, cell area, and focal adhesion number in hASCs. Moreover, the integration of the ND-Dex complex within GelMA hydrogels allowed a higher retention of Dex over time, resulting in significantly increased alkaline phosphatase activity and calcium deposition of encapsulated hASCs. These results suggest that conventional GelMA hydrogels can be coupled with conjugated NDs to develop a novel platform for bone tissue engineering.Settimio PacelliRyan MaloneyAparna R. ChakravartiJonathan WhitlowSayantani BasuSaman ModaresiStevin GehrkeArghya PaulNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Settimio Pacelli Ryan Maloney Aparna R. Chakravarti Jonathan Whitlow Sayantani Basu Saman Modaresi Stevin Gehrke Arghya Paul Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
description	Abstract Nanodiamonds (NDs) have attracted considerable attention as drug delivery nanocarriers due to their low cytotoxicity and facile surface functionalization. Given these features, NDs have been recently investigated for the fabrication of nanocomposite hydrogels for tissue engineering. Here we report the synthesis of a hydrogel using photocrosslinkable gelatin methacrylamide (GelMA) and NDs as a three-dimensional scaffold for drug delivery and stem cell-guided bone regeneration. We investigated the effect of different concentration of NDs on the physical and mechanical properties of the GelMA hydrogel network. The inclusion of NDs increased the network stiffness, which in turn augmented the traction forces generated by human adipose stem cells (hASCs). We also tested the ability of NDs to adsorb and modulate the release of a model drug dexamethasone (Dex) to promote the osteogenic differentiation of hASCs. The ND-Dex complexes modulated gene expression, cell area, and focal adhesion number in hASCs. Moreover, the integration of the ND-Dex complex within GelMA hydrogels allowed a higher retention of Dex over time, resulting in significantly increased alkaline phosphatase activity and calcium deposition of encapsulated hASCs. These results suggest that conventional GelMA hydrogels can be coupled with conjugated NDs to develop a novel platform for bone tissue engineering.
format	article
author	Settimio Pacelli Ryan Maloney Aparna R. Chakravarti Jonathan Whitlow Sayantani Basu Saman Modaresi Stevin Gehrke Arghya Paul
author_facet	Settimio Pacelli Ryan Maloney Aparna R. Chakravarti Jonathan Whitlow Sayantani Basu Saman Modaresi Stevin Gehrke Arghya Paul
author_sort	Settimio Pacelli
title	Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
title_short	Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
title_full	Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
title_fullStr	Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
title_full_unstemmed	Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy
title_sort	controlling adult stem cell behavior using nanodiamond-reinforced hydrogel: implication in bone regeneration therapy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/419b8269cf4944669490cb1ab703329e
work_keys_str_mv	AT settimiopacelli controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT ryanmaloney controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT aparnarchakravarti controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT jonathanwhitlow controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT sayantanibasu controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT samanmodaresi controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT stevingehrke controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy AT arghyapaul controllingadultstemcellbehaviorusingnanodiamondreinforcedhydrogelimplicationinboneregenerationtherapy
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Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy

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