A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.

Serine protease inhibitors, or serpins, are a group of widely distributed proteins with similar structures that use conformational change to inhibit proteases. Antithrombin (AT) is a member of the serine protease inhibitor superfamily and a major coagulation inhibitor in all vertebrates, but its evo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yeqing Chao, Chunxin Fan, Yujun Liang, Bei Gao, Shicui Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/419c58e65eec4bb99dcd1f54316a1bed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:419c58e65eec4bb99dcd1f54316a1bed
record_format	dspace
spelling	oai:doaj.org-article:419c58e65eec4bb99dcd1f54316a1bed2021-11-18T07:25:34ZA novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.1932-620310.1371/journal.pone.0032392https://doaj.org/article/419c58e65eec4bb99dcd1f54316a1bed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22427833/?tool=EBIhttps://doaj.org/toc/1932-6203Serine protease inhibitors, or serpins, are a group of widely distributed proteins with similar structures that use conformational change to inhibit proteases. Antithrombin (AT) is a member of the serine protease inhibitor superfamily and a major coagulation inhibitor in all vertebrates, but its evolutionary origin remains elusive. In this study we isolated for the first time a cDNA encoding an antithrombin homolog, BjATl, from the protochordate Branchiostoma japonicum. The deduced protein BjATl consisted of 338 amino acids sharing 36.7% to 41.1% identity to known vertebrate ATs. BjATl contains a potential N-linked glycosylation site, two potential heparin binding sites and the reactive center loop with the absolutely conserved sequence Gly-Arg-Ser; all of these are features characteristic of ATs. All three phylogenetic trees constructed using Neighbor-Joining, Maximum-Likelihood and Bayesian-Inference methods also placed BjATl together with ATs. Moreover, BjATl expressed in yeast cells was able to inhibit bovine thrombin activity by forming a SDS-stable BjATl-thrombin complex. It also displays a concentration-dependent inhibition of thrombin that is accelerated by heparin. Furthermore, BjATl was predominantly expressed in the hepatic caecum and hind-gut, agreeing with the expression pattern of AT in mammalian species. All these data clearly demonstrate that BjATl is an ortholog of vertebrate ATs, suggesting that a primitive coagulation system emerged in the protochordate.Yeqing ChaoChunxin FanYujun LiangBei GaoShicui ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32392 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yeqing Chao Chunxin Fan Yujun Liang Bei Gao Shicui Zhang A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
description	Serine protease inhibitors, or serpins, are a group of widely distributed proteins with similar structures that use conformational change to inhibit proteases. Antithrombin (AT) is a member of the serine protease inhibitor superfamily and a major coagulation inhibitor in all vertebrates, but its evolutionary origin remains elusive. In this study we isolated for the first time a cDNA encoding an antithrombin homolog, BjATl, from the protochordate Branchiostoma japonicum. The deduced protein BjATl consisted of 338 amino acids sharing 36.7% to 41.1% identity to known vertebrate ATs. BjATl contains a potential N-linked glycosylation site, two potential heparin binding sites and the reactive center loop with the absolutely conserved sequence Gly-Arg-Ser; all of these are features characteristic of ATs. All three phylogenetic trees constructed using Neighbor-Joining, Maximum-Likelihood and Bayesian-Inference methods also placed BjATl together with ATs. Moreover, BjATl expressed in yeast cells was able to inhibit bovine thrombin activity by forming a SDS-stable BjATl-thrombin complex. It also displays a concentration-dependent inhibition of thrombin that is accelerated by heparin. Furthermore, BjATl was predominantly expressed in the hepatic caecum and hind-gut, agreeing with the expression pattern of AT in mammalian species. All these data clearly demonstrate that BjATl is an ortholog of vertebrate ATs, suggesting that a primitive coagulation system emerged in the protochordate.
format	article
author	Yeqing Chao Chunxin Fan Yujun Liang Bei Gao Shicui Zhang
author_facet	Yeqing Chao Chunxin Fan Yujun Liang Bei Gao Shicui Zhang
author_sort	Yeqing Chao
title	A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
title_short	A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
title_full	A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
title_fullStr	A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
title_full_unstemmed	A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.
title_sort	novel serpin with antithrombin-like activity in branchiostoma japonicum: implications for the presence of a primitive coagulation system.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/419c58e65eec4bb99dcd1f54316a1bed
work_keys_str_mv	AT yeqingchao anovelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT chunxinfan anovelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT yujunliang anovelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT beigao anovelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT shicuizhang anovelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT yeqingchao novelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT chunxinfan novelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT yujunliang novelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT beigao novelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem AT shicuizhang novelserpinwithantithrombinlikeactivityinbranchiostomajaponicumimplicationsforthepresenceofaprimitivecoagulationsystem
_version_	1718423468689588224

A novel serpin with antithrombin-like activity in Branchiostoma japonicum: implications for the presence of a primitive coagulation system.

Ejemplares similares