Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells

Abstract Smooth muscle cells (SMCs) are critical players in cardiovascular disease development and undergo complex phenotype switching during disease progression. However, SMC phenotype is difficult to assess and track in co-culture studies. To determine the contractility of SMCs embedded within col...

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Autores principales:	Olga Chashchina, Hachem Mezouar, Jérémy Vizet, Clothilde Raoux, Junha Park, Clara Ramón-Lozano, Marie-Claire Schanne-Klein, Abdul I. Barakat, Angelo Pierangelo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41b06b3a34b74f1bba340fd9dd66bfda
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spelling	oai:doaj.org-article:41b06b3a34b74f1bba340fd9dd66bfda2021-12-02T16:30:37ZMueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells10.1038/s41598-021-85164-y2045-2322https://doaj.org/article/41b06b3a34b74f1bba340fd9dd66bfda2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85164-yhttps://doaj.org/toc/2045-2322Abstract Smooth muscle cells (SMCs) are critical players in cardiovascular disease development and undergo complex phenotype switching during disease progression. However, SMC phenotype is difficult to assess and track in co-culture studies. To determine the contractility of SMCs embedded within collagen hydrogels, we performed polarized light imaging and subsequent analysis based on Mueller matrices. Measurements were made both in the absence and presence of endothelial cells (ECs) in order to establish the impact of EC-SMC communication on SMC contractility. The results demonstrated that Mueller polarimetric imaging is indeed an appropriate tool for assessing SMC activity which significantly modifies the hydrogel retardance in the presence of ECs. These findings are consistent with the idea that EC-SMC communication promotes a more contractile SMC phenotype. More broadly, our findings suggest that Mueller polarimetry can be a useful tool for studies of spatial heterogeneities in hydrogel remodeling by SMCs.Olga ChashchinaHachem MezouarJérémy VizetClothilde RaouxJunha ParkClara Ramón-LozanoMarie-Claire Schanne-KleinAbdul I. BarakatAngelo PierangeloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olga Chashchina Hachem Mezouar Jérémy Vizet Clothilde Raoux Junha Park Clara Ramón-Lozano Marie-Claire Schanne-Klein Abdul I. Barakat Angelo Pierangelo Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
description	Abstract Smooth muscle cells (SMCs) are critical players in cardiovascular disease development and undergo complex phenotype switching during disease progression. However, SMC phenotype is difficult to assess and track in co-culture studies. To determine the contractility of SMCs embedded within collagen hydrogels, we performed polarized light imaging and subsequent analysis based on Mueller matrices. Measurements were made both in the absence and presence of endothelial cells (ECs) in order to establish the impact of EC-SMC communication on SMC contractility. The results demonstrated that Mueller polarimetric imaging is indeed an appropriate tool for assessing SMC activity which significantly modifies the hydrogel retardance in the presence of ECs. These findings are consistent with the idea that EC-SMC communication promotes a more contractile SMC phenotype. More broadly, our findings suggest that Mueller polarimetry can be a useful tool for studies of spatial heterogeneities in hydrogel remodeling by SMCs.
format	article
author	Olga Chashchina Hachem Mezouar Jérémy Vizet Clothilde Raoux Junha Park Clara Ramón-Lozano Marie-Claire Schanne-Klein Abdul I. Barakat Angelo Pierangelo
author_facet	Olga Chashchina Hachem Mezouar Jérémy Vizet Clothilde Raoux Junha Park Clara Ramón-Lozano Marie-Claire Schanne-Klein Abdul I. Barakat Angelo Pierangelo
author_sort	Olga Chashchina
title	Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
title_short	Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
title_full	Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
title_fullStr	Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
title_full_unstemmed	Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
title_sort	mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/41b06b3a34b74f1bba340fd9dd66bfda
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_version_	1718383925763506176

Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells

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