Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.

Endothelial cell migration is an essential aspect of tumor angiogenesis. Rac1 activity is needed for cell migration in vitro implying a requirement for this molecule in angiogenesis in vivo. However, a precise role for Rac1 in tumor angiogenesis has never been addressed. Here we show that depletion...

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Autores principales:	Gabriela D'Amico, Stephen D Robinson, Mitchel Germain, Louise E Reynolds, Gareth J Thomas, George Elia, Garry Saunders, Marcus Fruttiger, Victor Tybulewicz, Georgia Mavria, Kairbaan M Hodivala-Dilke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41b0c7cc9f614c6dbf5291476797a248
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spelling	oai:doaj.org-article:41b0c7cc9f614c6dbf5291476797a2482021-11-25T06:25:18ZEndothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.1932-620310.1371/journal.pone.0009766https://doaj.org/article/41b0c7cc9f614c6dbf5291476797a2482010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20339539/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Endothelial cell migration is an essential aspect of tumor angiogenesis. Rac1 activity is needed for cell migration in vitro implying a requirement for this molecule in angiogenesis in vivo. However, a precise role for Rac1 in tumor angiogenesis has never been addressed. Here we show that depletion of endothelial Rac1 expression in adult mice, unexpectedly, has no effect on tumor growth or tumor angiogenesis. In addition, repression of Rac1 expression does not inhibit VEGF-mediated angiogenesis in vivo or ex vivo, nor does it affect chemotactic migratory responses to VEGF in 3-dimensions. In contrast, the requirement for Rac1 in tumor growth and angiogenesis becomes important when endothelial beta3-integrin levels are reduced or absent: the enhanced tumor growth, tumor angiogenesis and VEGF-mediated responses in beta3-null mice are all Rac1-dependent. These data indicate that in the presence of alphavbeta3-integrin Rac1 is not required for tumor angiogenesis.Gabriela D'AmicoStephen D RobinsonMitchel GermainLouise E ReynoldsGareth J ThomasGeorge EliaGarry SaundersMarcus FruttigerVictor TybulewiczGeorgia MavriaKairbaan M Hodivala-DilkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 3, p e9766 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriela D'Amico Stephen D Robinson Mitchel Germain Louise E Reynolds Gareth J Thomas George Elia Garry Saunders Marcus Fruttiger Victor Tybulewicz Georgia Mavria Kairbaan M Hodivala-Dilke Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
description	Endothelial cell migration is an essential aspect of tumor angiogenesis. Rac1 activity is needed for cell migration in vitro implying a requirement for this molecule in angiogenesis in vivo. However, a precise role for Rac1 in tumor angiogenesis has never been addressed. Here we show that depletion of endothelial Rac1 expression in adult mice, unexpectedly, has no effect on tumor growth or tumor angiogenesis. In addition, repression of Rac1 expression does not inhibit VEGF-mediated angiogenesis in vivo or ex vivo, nor does it affect chemotactic migratory responses to VEGF in 3-dimensions. In contrast, the requirement for Rac1 in tumor growth and angiogenesis becomes important when endothelial beta3-integrin levels are reduced or absent: the enhanced tumor growth, tumor angiogenesis and VEGF-mediated responses in beta3-null mice are all Rac1-dependent. These data indicate that in the presence of alphavbeta3-integrin Rac1 is not required for tumor angiogenesis.
format	article
author	Gabriela D'Amico Stephen D Robinson Mitchel Germain Louise E Reynolds Gareth J Thomas George Elia Garry Saunders Marcus Fruttiger Victor Tybulewicz Georgia Mavria Kairbaan M Hodivala-Dilke
author_facet	Gabriela D'Amico Stephen D Robinson Mitchel Germain Louise E Reynolds Gareth J Thomas George Elia Garry Saunders Marcus Fruttiger Victor Tybulewicz Georgia Mavria Kairbaan M Hodivala-Dilke
author_sort	Gabriela D'Amico
title	Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
title_short	Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
title_full	Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
title_fullStr	Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
title_full_unstemmed	Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
title_sort	endothelial-rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/41b0c7cc9f614c6dbf5291476797a248
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Endothelial-Rac1 is not required for tumor angiogenesis unless alphavbeta3-integrin is absent.

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