mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions

ABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organ...

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Autor principal:	Calvin Tiengwe
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	AGPC OOPS RNA binding proteins Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/41c4991d564f46c080f5efa01de5723e
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spelling	oai:doaj.org-article:41c4991d564f46c080f5efa01de5723e2021-11-15T15:27:33ZmSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions10.1128/mSphere.00600-192379-5042https://doaj.org/article/41c4991d564f46c080f5efa01de5723e2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00600-19https://doaj.org/toc/2379-5042ABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)” by Queiroz et al. (Nat Biotechnol 37:169–178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.Calvin TiengweAmerican Society for MicrobiologyarticleAGPCOOPSRNA binding proteinsMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	AGPC OOPS RNA binding proteins Microbiology QR1-502
spellingShingle	AGPC OOPS RNA binding proteins Microbiology QR1-502 Calvin Tiengwe mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
description	ABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)” by Queiroz et al. (Nat Biotechnol 37:169–178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.
format	article
author	Calvin Tiengwe
author_facet	Calvin Tiengwe
author_sort	Calvin Tiengwe
title	mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
title_short	mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
title_full	mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
title_fullStr	mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
title_full_unstemmed	mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
title_sort	msphere of influence: modifying an old method to study rna-protein interactions
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/41c4991d564f46c080f5efa01de5723e
work_keys_str_mv	AT calvintiengwe msphereofinfluencemodifyinganoldmethodtostudyrnaproteininteractions
_version_	1718427943682703360

mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions

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