Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection

Wei Wang,1 Fang Zhou,2 Linfu Ge,2 Ximin Liu,2 Fansheng Kong21Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji&rsquo;nan Command, Ji&rsquo;nan, China; 2Department of Hematology, General Hospital of Ji&rsquo;nan...

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Autores principales:	Wang W, Zhou F, Ge L, Liu X, Kong F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/41c826aa7a4a4a7c922964b1843330bd
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spelling	oai:doaj.org-article:41c826aa7a4a4a7c922964b1843330bd2021-12-02T02:16:44ZTransferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection1176-91141178-2013https://doaj.org/article/41c826aa7a4a4a7c922964b1843330bd2012-05-01T00:00:00Zhttp://www.dovepress.com/transferrin-peg-pe-modified-dexamethasone-conjugated-cationic-lipid-ca-a9928https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Wei Wang,1 Fang Zhou,2 Linfu Ge,2 Ximin Liu,2 Fansheng Kong21Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji&rsquo;nan Command, Ji&rsquo;nan, China; 2Department of Hematology, General Hospital of Ji&rsquo;nan Command, Ji&rsquo;nan, ChinaBackground: The main barriers to non-viral gene delivery include cellular and nuclear membranes. As such, the aim of this study was to develop a type of vector that can target cells through receptor-mediated pathways and by using nuclear localization signal (NLS) to increase the nuclear uptake of genetic materials.Methods: A dexamethasone (Dexa)-conjugated lipid was synthesized as the material of the solid lipid nanoparticles (SLNs), and transferrin (Tf) was linked onto polyethylene glycol-phosphatidylethanolamine (PEG-PE) to obtain Tf-PEG-PE ligands for the surface modification of the carriers. The in vitro transfection efficiency of the novel modified vectors was evaluated in human hepatoma carcinoma cell lines, and in vivo effects were observed in an animal model.Results: Tf-PEG-PE modified SLNs/enhanced green fluorescence protein plasmid (pEGFP) had a particle size of 222 nm and a gene loading quantity of 90%. Tf-PEG-PE-modified SLNs/pEGFP (Tf-SLNs/pEGFP) displayed remarkably higher transfection efficiency than non-modified SLNs/pEGFP and the vectors not containing Dexa, both in vitro and in vivo.Conclusion: It can be concluded that Tf and Dexa could function as an excellent active targeting ligand to improve the cell targeting and nuclear targeting ability of the carriers, and the resulting nanomedicine could be a promising active targeting drug/gene delivery system.Keywords: gene delivery, active targeting, transferrin-PEG-PE, dexamethasone conjugated lipid, nuclear localizationWang WZhou FGe LLiu XKong FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2513-2522 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang W Zhou F Ge L Liu X Kong F Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
description	Wei Wang,1 Fang Zhou,2 Linfu Ge,2 Ximin Liu,2 Fansheng Kong21Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji&rsquo;nan Command, Ji&rsquo;nan, China; 2Department of Hematology, General Hospital of Ji&rsquo;nan Command, Ji&rsquo;nan, ChinaBackground: The main barriers to non-viral gene delivery include cellular and nuclear membranes. As such, the aim of this study was to develop a type of vector that can target cells through receptor-mediated pathways and by using nuclear localization signal (NLS) to increase the nuclear uptake of genetic materials.Methods: A dexamethasone (Dexa)-conjugated lipid was synthesized as the material of the solid lipid nanoparticles (SLNs), and transferrin (Tf) was linked onto polyethylene glycol-phosphatidylethanolamine (PEG-PE) to obtain Tf-PEG-PE ligands for the surface modification of the carriers. The in vitro transfection efficiency of the novel modified vectors was evaluated in human hepatoma carcinoma cell lines, and in vivo effects were observed in an animal model.Results: Tf-PEG-PE modified SLNs/enhanced green fluorescence protein plasmid (pEGFP) had a particle size of 222 nm and a gene loading quantity of 90%. Tf-PEG-PE-modified SLNs/pEGFP (Tf-SLNs/pEGFP) displayed remarkably higher transfection efficiency than non-modified SLNs/pEGFP and the vectors not containing Dexa, both in vitro and in vivo.Conclusion: It can be concluded that Tf and Dexa could function as an excellent active targeting ligand to improve the cell targeting and nuclear targeting ability of the carriers, and the resulting nanomedicine could be a promising active targeting drug/gene delivery system.Keywords: gene delivery, active targeting, transferrin-PEG-PE, dexamethasone conjugated lipid, nuclear localization
format	article
author	Wang W Zhou F Ge L Liu X Kong F
author_facet	Wang W Zhou F Ge L Liu X Kong F
author_sort	Wang W
title	Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
title_short	Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
title_full	Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
title_fullStr	Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
title_full_unstemmed	Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
title_sort	transferrin-peg-pe modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/41c826aa7a4a4a7c922964b1843330bd
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Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection

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