Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation

Abstract Background Fundamental knowledge of cellular and molecular mechanisms in developing testicular tissues is critical to better understand gonadal biology and responses to non-physiological conditions. The objective of our study was to (1) analyze transcriptome dynamics in developing testis of...

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Autores principales:	Olga Amelkina, Andreia M. da Silva, Alexandre R. Silva, Pierre Comizzoli
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Puberty Vitrification Testicular tissue Domestic cat RNA-seq Networks Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/41d448e172234639a263f2643f44a7da
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spelling	oai:doaj.org-article:41d448e172234639a263f2643f44a7da2021-11-28T12:23:02ZTranscriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation10.1186/s12864-021-08099-81471-2164https://doaj.org/article/41d448e172234639a263f2643f44a7da2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08099-8https://doaj.org/toc/1471-2164Abstract Background Fundamental knowledge of cellular and molecular mechanisms in developing testicular tissues is critical to better understand gonadal biology and responses to non-physiological conditions. The objective of our study was to (1) analyze transcriptome dynamics in developing testis of the domestic cat and (2) characterize age effects on the initial response of the tissue to vitrification. Tissues from adult and juvenile cats were processed for histology, DNA integrity, and RNA sequencing analyses before and after vitrification. Results Transcriptomic findings enabled to further characterize juvenile period, distinguishing between early and late juvenile tissues. Changes in gene expression and functional pathways were extensive from early to late juvenile to adult development stages. Additionally, tissues from juvenile animals were more resilient to vitrification compared to adult counterparts, with early juvenile sample responding the least to vitrification and late juvenile sample response being closest to adult tissues. Conclusions This is the first study reporting comprehensive datasets on transcriptomic dynamic coupled with structural analysis of the cat testis according to the age and exposure to cryopreservation. It provides a comprehensive network of functional terms and pathways that are affected by age in the domestic cat and are either enriched in adult or juvenile testicular tissues.Olga AmelkinaAndreia M. da SilvaAlexandre R. SilvaPierre ComizzoliBMCarticlePubertyVitrificationTesticular tissueDomestic catRNA-seqNetworksBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Puberty Vitrification Testicular tissue Domestic cat RNA-seq Networks Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Puberty Vitrification Testicular tissue Domestic cat RNA-seq Networks Biotechnology TP248.13-248.65 Genetics QH426-470 Olga Amelkina Andreia M. da Silva Alexandre R. Silva Pierre Comizzoli Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
description	Abstract Background Fundamental knowledge of cellular and molecular mechanisms in developing testicular tissues is critical to better understand gonadal biology and responses to non-physiological conditions. The objective of our study was to (1) analyze transcriptome dynamics in developing testis of the domestic cat and (2) characterize age effects on the initial response of the tissue to vitrification. Tissues from adult and juvenile cats were processed for histology, DNA integrity, and RNA sequencing analyses before and after vitrification. Results Transcriptomic findings enabled to further characterize juvenile period, distinguishing between early and late juvenile tissues. Changes in gene expression and functional pathways were extensive from early to late juvenile to adult development stages. Additionally, tissues from juvenile animals were more resilient to vitrification compared to adult counterparts, with early juvenile sample responding the least to vitrification and late juvenile sample response being closest to adult tissues. Conclusions This is the first study reporting comprehensive datasets on transcriptomic dynamic coupled with structural analysis of the cat testis according to the age and exposure to cryopreservation. It provides a comprehensive network of functional terms and pathways that are affected by age in the domestic cat and are either enriched in adult or juvenile testicular tissues.
format	article
author	Olga Amelkina Andreia M. da Silva Alexandre R. Silva Pierre Comizzoli
author_facet	Olga Amelkina Andreia M. da Silva Alexandre R. Silva Pierre Comizzoli
author_sort	Olga Amelkina
title	Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
title_short	Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
title_full	Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
title_fullStr	Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
title_full_unstemmed	Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
title_sort	transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation
publisher	BMC
publishDate	2021
url	https://doaj.org/article/41d448e172234639a263f2643f44a7da
work_keys_str_mv	AT olgaamelkina transcriptomedynamicsindevelopingtestesofdomesticcatsandimpactofageontissueresiliencetocryopreservation AT andreiamdasilva transcriptomedynamicsindevelopingtestesofdomesticcatsandimpactofageontissueresiliencetocryopreservation AT alexandrersilva transcriptomedynamicsindevelopingtestesofdomesticcatsandimpactofageontissueresiliencetocryopreservation AT pierrecomizzoli transcriptomedynamicsindevelopingtestesofdomesticcatsandimpactofageontissueresiliencetocryopreservation
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Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation

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