Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis

Abstract The beet cyst nematode Heterodera schachtii causes major yield losses in sugar beet. Understanding the interaction between H. schachtii and its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining succe...

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Autores principales:	Samer S. Habash, Zoran S. Radakovic, Radomira Vankova, Shahid Siddique, Petre Dobrev, Cynthia Gleason, Florian M. W. Grundler, Abdelnaser Elashry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41d7024bf379468d85621cf062632f5f
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spelling	oai:doaj.org-article:41d7024bf379468d85621cf062632f5f2021-12-02T12:31:47ZHeterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis10.1038/s41598-017-07269-72045-2322https://doaj.org/article/41d7024bf379468d85621cf062632f5f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07269-7https://doaj.org/toc/2045-2322Abstract The beet cyst nematode Heterodera schachtii causes major yield losses in sugar beet. Understanding the interaction between H. schachtii and its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining successful parasitism. In our study, we identified a gene (Hs-Tyr) encoding a tyrosinase functional domain (PF00264). We describe Hs-Tyr as a novel nematode effector. Hs-Tyr is localized in the nematode esophageal gland. Up-regulation of its expression coincided with the parasitic developmental stages of the nematode. Silencing Hs-Tyr by RNA interference made the treated nematodes less virulent. When RNAi-treated nematodes succeeded in infecting the plant, developing females and their associated syncytial nurse cells were significantly smaller than in control plants. Ectopically expressing the Hs-Tyr effector in Arabidopsis increased plant susceptibility to H. schachtii, but not to the root-knot nematode Meloidogyne incognita. Interestingly, Hs-Tyr in the plant promoted plant growth and changed the root architecture. Additionally, the expression of Hs-Tyr in Arabidopsis caused changes in the homeostasis of several plant hormones especially auxin and the ethylene precursor aminocyclopropane-carboxylic acid.Samer S. HabashZoran S. RadakovicRadomira VankovaShahid SiddiquePetre DobrevCynthia GleasonFlorian M. W. GrundlerAbdelnaser ElashryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samer S. Habash Zoran S. Radakovic Radomira Vankova Shahid Siddique Petre Dobrev Cynthia Gleason Florian M. W. Grundler Abdelnaser Elashry Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
description	Abstract The beet cyst nematode Heterodera schachtii causes major yield losses in sugar beet. Understanding the interaction between H. schachtii and its host plant is important for developing a sustainable management system. Nematode effectors play a crucial role in initializing and sustaining successful parasitism. In our study, we identified a gene (Hs-Tyr) encoding a tyrosinase functional domain (PF00264). We describe Hs-Tyr as a novel nematode effector. Hs-Tyr is localized in the nematode esophageal gland. Up-regulation of its expression coincided with the parasitic developmental stages of the nematode. Silencing Hs-Tyr by RNA interference made the treated nematodes less virulent. When RNAi-treated nematodes succeeded in infecting the plant, developing females and their associated syncytial nurse cells were significantly smaller than in control plants. Ectopically expressing the Hs-Tyr effector in Arabidopsis increased plant susceptibility to H. schachtii, but not to the root-knot nematode Meloidogyne incognita. Interestingly, Hs-Tyr in the plant promoted plant growth and changed the root architecture. Additionally, the expression of Hs-Tyr in Arabidopsis caused changes in the homeostasis of several plant hormones especially auxin and the ethylene precursor aminocyclopropane-carboxylic acid.
format	article
author	Samer S. Habash Zoran S. Radakovic Radomira Vankova Shahid Siddique Petre Dobrev Cynthia Gleason Florian M. W. Grundler Abdelnaser Elashry
author_facet	Samer S. Habash Zoran S. Radakovic Radomira Vankova Shahid Siddique Petre Dobrev Cynthia Gleason Florian M. W. Grundler Abdelnaser Elashry
author_sort	Samer S. Habash
title	Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
title_short	Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
title_full	Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
title_fullStr	Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
title_full_unstemmed	Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
title_sort	heterodera schachtii tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/41d7024bf379468d85621cf062632f5f
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Heterodera schachtii Tyrosinase-like protein - a novel nematode effector modulating plant hormone homeostasis

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