Surpassing light-induced cell damage in vitro with novel cell culture media

Abstract Light is extensively used to study cells in real time (live cell imaging), separate cells using fluorescence activated cell sorting (FACS) and control cellular functions with light sensitive proteins (Optogenetics). However, photo-sensitive molecules inside cells and in standard cell cultur...

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Autores principales:	John H. Stockley, Kimberley Evans, Moritz Matthey, Katrin Volbracht, Sylvia Agathou, Jana Mukanowa, Juan Burrone, Ragnhildur T. Káradóttir
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41da7c54bdf74c809844693e99deb927
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spelling	oai:doaj.org-article:41da7c54bdf74c809844693e99deb9272021-12-02T16:06:41ZSurpassing light-induced cell damage in vitro with novel cell culture media10.1038/s41598-017-00829-x2045-2322https://doaj.org/article/41da7c54bdf74c809844693e99deb9272017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00829-xhttps://doaj.org/toc/2045-2322Abstract Light is extensively used to study cells in real time (live cell imaging), separate cells using fluorescence activated cell sorting (FACS) and control cellular functions with light sensitive proteins (Optogenetics). However, photo-sensitive molecules inside cells and in standard cell culture media generate toxic by-products that interfere with cellular functions and cell viability when exposed to light. Here we show that primary cells from the rat central nervous system respond differently to photo-toxicity, in that astrocytes and microglia undergo morphological changes, while in developing neurons and oligodendrocyte progenitor cells (OPCs) it induces cellular death. To prevent photo-toxicity and to allow for long-term photo-stimulation without causing cellular damage, we formulated new photo-inert media called MEMO and NEUMO, and an antioxidant rich and serum free supplement called SOS. These new media reduced the detrimental effects caused by light and allowed cells to endure up to twenty times more light exposure without adverse effects, thus bypassing the optical constraints previously limiting experiments.John H. StockleyKimberley EvansMoritz MattheyKatrin VolbrachtSylvia AgathouJana MukanowaJuan BurroneRagnhildur T. KáradóttirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John H. Stockley Kimberley Evans Moritz Matthey Katrin Volbracht Sylvia Agathou Jana Mukanowa Juan Burrone Ragnhildur T. Káradóttir Surpassing light-induced cell damage in vitro with novel cell culture media
description	Abstract Light is extensively used to study cells in real time (live cell imaging), separate cells using fluorescence activated cell sorting (FACS) and control cellular functions with light sensitive proteins (Optogenetics). However, photo-sensitive molecules inside cells and in standard cell culture media generate toxic by-products that interfere with cellular functions and cell viability when exposed to light. Here we show that primary cells from the rat central nervous system respond differently to photo-toxicity, in that astrocytes and microglia undergo morphological changes, while in developing neurons and oligodendrocyte progenitor cells (OPCs) it induces cellular death. To prevent photo-toxicity and to allow for long-term photo-stimulation without causing cellular damage, we formulated new photo-inert media called MEMO and NEUMO, and an antioxidant rich and serum free supplement called SOS. These new media reduced the detrimental effects caused by light and allowed cells to endure up to twenty times more light exposure without adverse effects, thus bypassing the optical constraints previously limiting experiments.
format	article
author	John H. Stockley Kimberley Evans Moritz Matthey Katrin Volbracht Sylvia Agathou Jana Mukanowa Juan Burrone Ragnhildur T. Káradóttir
author_facet	John H. Stockley Kimberley Evans Moritz Matthey Katrin Volbracht Sylvia Agathou Jana Mukanowa Juan Burrone Ragnhildur T. Káradóttir
author_sort	John H. Stockley
title	Surpassing light-induced cell damage in vitro with novel cell culture media
title_short	Surpassing light-induced cell damage in vitro with novel cell culture media
title_full	Surpassing light-induced cell damage in vitro with novel cell culture media
title_fullStr	Surpassing light-induced cell damage in vitro with novel cell culture media
title_full_unstemmed	Surpassing light-induced cell damage in vitro with novel cell culture media
title_sort	surpassing light-induced cell damage in vitro with novel cell culture media
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/41da7c54bdf74c809844693e99deb927
work_keys_str_mv	AT johnhstockley surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT kimberleyevans surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT moritzmatthey surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT katrinvolbracht surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT sylviaagathou surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT janamukanowa surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT juanburrone surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia AT ragnhildurtkaradottir surpassinglightinducedcelldamageinvitrowithnovelcellculturemedia
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Surpassing light-induced cell damage in vitro with novel cell culture media

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