Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine

Development of low-cost and eco-friendly approaches to fight bacterial pathogens is especially needed in aquaculture. We previously showed that exogenous malate reprograms zebrafish’s metabolome to potentiate zebrafish survival against Vibrio alginolyticus infection. However, the underlying mechanis...

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Autores principales: Man-Jun Yang, Di Xu, Dai-Xiao Yang, Lu Li, Xuan-Xian Peng, Zhuang-gui Chen, Hui Li
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Lenguaje:EN
Publicado: Taylor & Francis Group 2020
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Acceso en línea:https://doaj.org/article/41e0273f3a0a41c9b2ea50dad296ca43
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spelling oai:doaj.org-article:41e0273f3a0a41c9b2ea50dad296ca432021-11-17T14:21:58ZMalate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine2150-55942150-560810.1080/21505594.2020.1750123https://doaj.org/article/41e0273f3a0a41c9b2ea50dad296ca432020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1750123https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Development of low-cost and eco-friendly approaches to fight bacterial pathogens is especially needed in aquaculture. We previously showed that exogenous malate reprograms zebrafish’s metabolome to potentiate zebrafish survival against Vibrio alginolyticus infection. However, the underlying mechanism is unknown. Here, we use GC-MS based metabolomics to identify the malate-triggered metabolic shift. An activated TCA cycle and elevated taurine are identified as the key metabolic pathways and the most crucial biomarker of the reprogrammed metabolome, respectively. Taurine elevation is attributed to the activated TCA cycle, which is further supported by the increased expression of genes in the metabolic pathway of taurine biosynthesis from the isocitrate of the TCA cycle to taurine. Exogenous taurine increases the survival of zebrafish against V. alginolyticus infection as malate did. Moreover, exogenous taurine and malate regulate the expression of innate immunity genes and promote the generation of reactive oxygen species and nitrogen oxide in a similar way. The two metabolites can alleviate the excessive immune response to bacterial challenge, which protects fish from bacterial infection. These results indicate that malate enhances the survival of zebrafish to V. alginolyticus infection via taurine. Thus, our study highlights a metabolic approach to enhance a host’s ability to fight bacterial infection.Man-Jun YangDi XuDai-Xiao YangLu LiXuan-Xian PengZhuang-gui ChenHui LiTaylor & Francis Grouparticletaurinemalatereprogramming metabolomicsvibrio alginolyticusaquaculturezebrafishInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 349-364 (2020)
institution DOAJ
collection DOAJ
language EN
topic taurine
malate
reprogramming metabolomics
vibrio alginolyticus
aquaculture
zebrafish
Infectious and parasitic diseases
RC109-216
spellingShingle taurine
malate
reprogramming metabolomics
vibrio alginolyticus
aquaculture
zebrafish
Infectious and parasitic diseases
RC109-216
Man-Jun Yang
Di Xu
Dai-Xiao Yang
Lu Li
Xuan-Xian Peng
Zhuang-gui Chen
Hui Li
Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
description Development of low-cost and eco-friendly approaches to fight bacterial pathogens is especially needed in aquaculture. We previously showed that exogenous malate reprograms zebrafish’s metabolome to potentiate zebrafish survival against Vibrio alginolyticus infection. However, the underlying mechanism is unknown. Here, we use GC-MS based metabolomics to identify the malate-triggered metabolic shift. An activated TCA cycle and elevated taurine are identified as the key metabolic pathways and the most crucial biomarker of the reprogrammed metabolome, respectively. Taurine elevation is attributed to the activated TCA cycle, which is further supported by the increased expression of genes in the metabolic pathway of taurine biosynthesis from the isocitrate of the TCA cycle to taurine. Exogenous taurine increases the survival of zebrafish against V. alginolyticus infection as malate did. Moreover, exogenous taurine and malate regulate the expression of innate immunity genes and promote the generation of reactive oxygen species and nitrogen oxide in a similar way. The two metabolites can alleviate the excessive immune response to bacterial challenge, which protects fish from bacterial infection. These results indicate that malate enhances the survival of zebrafish to V. alginolyticus infection via taurine. Thus, our study highlights a metabolic approach to enhance a host’s ability to fight bacterial infection.
format article
author Man-Jun Yang
Di Xu
Dai-Xiao Yang
Lu Li
Xuan-Xian Peng
Zhuang-gui Chen
Hui Li
author_facet Man-Jun Yang
Di Xu
Dai-Xiao Yang
Lu Li
Xuan-Xian Peng
Zhuang-gui Chen
Hui Li
author_sort Man-Jun Yang
title Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
title_short Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
title_full Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
title_fullStr Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
title_full_unstemmed Malate enhances survival of zebrafish against Vibrio alginolyticus infection in the same manner as taurine
title_sort malate enhances survival of zebrafish against vibrio alginolyticus infection in the same manner as taurine
publisher Taylor & Francis Group
publishDate 2020
url https://doaj.org/article/41e0273f3a0a41c9b2ea50dad296ca43
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