The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria

Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative s...

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Autores principales:	Lavinia Gambelli, Rob Mesman, Wouter Versantvoort, Christoph A. Diebolder, Andreas Engel, Wiel Evers, Mike S. M. Jetten, Martin Pabst, Bertram Daum, Laura van Niftrik
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Methylomirabilis NC10 phylum anaerobic methane oxidation S-layer cell shape cryo-tomography Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/41e14abb9c5d4abfbcaf7de17873aac5
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spelling	oai:doaj.org-article:41e14abb9c5d4abfbcaf7de17873aac52021-12-01T23:11:12ZThe Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria1664-302X10.3389/fmicb.2021.766527https://doaj.org/article/41e14abb9c5d4abfbcaf7de17873aac52021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.766527/fullhttps://doaj.org/toc/1664-302XMethylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative surface protein layer (S-layer) was observed as the outermost cell layer of these bacteria. We hypothesized that this S-layer is the determining factor for their polygonal cell shape. Therefore, we enriched the S-layer from M. lanthanidiphila cells and through LC-MS/MS identified a 31 kDa candidate S-layer protein, mela_00855, which had no homology to any other known protein. Antibodies were generated against a synthesized peptide derived from the mela_00855 protein sequence and used in immunogold localization to verify its identity and location. Both on thin sections of M. lanthanidiphila cells and in negative-stained enriched S-layer patches, the immunogold localization identified mela_00855 as the S-layer protein. Using electron cryo-tomography and sub-tomogram averaging of S-layer patches, we observed that the S-layer has a hexagonal symmetry. Cryo-tomography of whole cells showed that the S-layer and the outer membrane, but not the peptidoglycan layer and the cytoplasmic membrane, exhibited the polygonal shape. Moreover, the S-layer consisted of multiple rigid sheets that partially overlapped, most likely giving rise to the unique polygonal cell shape. These characteristics make the S-layer of M. lanthanidiphila a distinctive and intriguing case to study.Lavinia GambelliLavinia GambelliLavinia GambelliRob MesmanWouter VersantvoortChristoph A. DiebolderAndreas EngelWiel EversWiel EversMike S. M. JettenMartin PabstBertram DaumBertram DaumLaura van NiftrikFrontiers Media S.A.articleMethylomirabilisNC10 phylumanaerobic methane oxidationS-layercell shapecryo-tomographyMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Methylomirabilis NC10 phylum anaerobic methane oxidation S-layer cell shape cryo-tomography Microbiology QR1-502
spellingShingle	Methylomirabilis NC10 phylum anaerobic methane oxidation S-layer cell shape cryo-tomography Microbiology QR1-502 Lavinia Gambelli Lavinia Gambelli Lavinia Gambelli Rob Mesman Wouter Versantvoort Christoph A. Diebolder Andreas Engel Wiel Evers Wiel Evers Mike S. M. Jetten Martin Pabst Bertram Daum Bertram Daum Laura van Niftrik The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
description	Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative surface protein layer (S-layer) was observed as the outermost cell layer of these bacteria. We hypothesized that this S-layer is the determining factor for their polygonal cell shape. Therefore, we enriched the S-layer from M. lanthanidiphila cells and through LC-MS/MS identified a 31 kDa candidate S-layer protein, mela_00855, which had no homology to any other known protein. Antibodies were generated against a synthesized peptide derived from the mela_00855 protein sequence and used in immunogold localization to verify its identity and location. Both on thin sections of M. lanthanidiphila cells and in negative-stained enriched S-layer patches, the immunogold localization identified mela_00855 as the S-layer protein. Using electron cryo-tomography and sub-tomogram averaging of S-layer patches, we observed that the S-layer has a hexagonal symmetry. Cryo-tomography of whole cells showed that the S-layer and the outer membrane, but not the peptidoglycan layer and the cytoplasmic membrane, exhibited the polygonal shape. Moreover, the S-layer consisted of multiple rigid sheets that partially overlapped, most likely giving rise to the unique polygonal cell shape. These characteristics make the S-layer of M. lanthanidiphila a distinctive and intriguing case to study.
format	article
author	Lavinia Gambelli Lavinia Gambelli Lavinia Gambelli Rob Mesman Wouter Versantvoort Christoph A. Diebolder Andreas Engel Wiel Evers Wiel Evers Mike S. M. Jetten Martin Pabst Bertram Daum Bertram Daum Laura van Niftrik
author_facet	Lavinia Gambelli Lavinia Gambelli Lavinia Gambelli Rob Mesman Wouter Versantvoort Christoph A. Diebolder Andreas Engel Wiel Evers Wiel Evers Mike S. M. Jetten Martin Pabst Bertram Daum Bertram Daum Laura van Niftrik
author_sort	Lavinia Gambelli
title	The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
title_short	The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
title_full	The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
title_fullStr	The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
title_full_unstemmed	The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria
title_sort	polygonal cell shape and surface protein layer of anaerobic methane-oxidizing methylomirabilislanthanidiphila bacteria
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/41e14abb9c5d4abfbcaf7de17873aac5
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The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria

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