A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2

Emerging infectious viruses have led to global advances in the development of specific and sensitive detection techniques. Viruses have an inherent potential to easily mutate, presenting major hurdles for diagnostics and requiring methods capable of detecting genetically diverse viral strains. One s...

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Autores principales:	Andreas C. Chrysostomou, Johana Hezka Rodosthenous, Cicek Topcu, Christina Papa, Antonia Aristokleous, Georgia Stathi, Christina Christodoulou, Christina Eleftheriou, Dora C. Stylianou, Leondios G. Kostrikis
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	COVID-19 SARS-CoV-2 molecular beacons Science Q
Acceso en línea:	https://doaj.org/article/41e2d50fe104486dac5120ed21c6bcf5
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spelling	oai:doaj.org-article:41e2d50fe104486dac5120ed21c6bcf52021-11-25T18:10:39ZA Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-210.3390/life111111462075-1729https://doaj.org/article/41e2d50fe104486dac5120ed21c6bcf52021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1146https://doaj.org/toc/2075-1729Emerging infectious viruses have led to global advances in the development of specific and sensitive detection techniques. Viruses have an inherent potential to easily mutate, presenting major hurdles for diagnostics and requiring methods capable of detecting genetically diverse viral strains. One such infectious agent is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in December 2019 and has resulted in the global coronavirus disease 2019 (COVID-19) pandemic. This study presents a real-time reverse transcription PCR (RT-PCR) detection assay for SARS-CoV-2, taking into account its intrinsic polymorphic nature that arises due to genetic drift and recombination, as well as the possibility of continuous and multiple introductions of genetically nonidentical strains into the human population. This advance was achieved by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV-2 S, E, M, and N genes. These were applied to create a simple and reproducible real-time RT-PCR assay, which was validated using external quality control panels (QCMD: CVOP20, WHO: SARS-CoV-2-EQAP-01) and clinical samples. This assay was designed for high target detection accuracy and specificity and can also be readily adapted for the detection of other emerging and rapidly mutating pathogens.Andreas C. ChrysostomouJohana Hezka RodosthenousCicek TopcuChristina PapaAntonia AristokleousGeorgia StathiChristina ChristodoulouChristina EleftheriouDora C. StylianouLeondios G. KostrikisMDPI AGarticleCOVID-19SARS-CoV-2molecular beaconsScienceQENLife, Vol 11, Iss 1146, p 1146 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 molecular beacons Science Q
spellingShingle	COVID-19 SARS-CoV-2 molecular beacons Science Q Andreas C. Chrysostomou Johana Hezka Rodosthenous Cicek Topcu Christina Papa Antonia Aristokleous Georgia Stathi Christina Christodoulou Christina Eleftheriou Dora C. Stylianou Leondios G. Kostrikis A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
description	Emerging infectious viruses have led to global advances in the development of specific and sensitive detection techniques. Viruses have an inherent potential to easily mutate, presenting major hurdles for diagnostics and requiring methods capable of detecting genetically diverse viral strains. One such infectious agent is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in December 2019 and has resulted in the global coronavirus disease 2019 (COVID-19) pandemic. This study presents a real-time reverse transcription PCR (RT-PCR) detection assay for SARS-CoV-2, taking into account its intrinsic polymorphic nature that arises due to genetic drift and recombination, as well as the possibility of continuous and multiple introductions of genetically nonidentical strains into the human population. This advance was achieved by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV-2 S, E, M, and N genes. These were applied to create a simple and reproducible real-time RT-PCR assay, which was validated using external quality control panels (QCMD: CVOP20, WHO: SARS-CoV-2-EQAP-01) and clinical samples. This assay was designed for high target detection accuracy and specificity and can also be readily adapted for the detection of other emerging and rapidly mutating pathogens.
format	article
author	Andreas C. Chrysostomou Johana Hezka Rodosthenous Cicek Topcu Christina Papa Antonia Aristokleous Georgia Stathi Christina Christodoulou Christina Eleftheriou Dora C. Stylianou Leondios G. Kostrikis
author_facet	Andreas C. Chrysostomou Johana Hezka Rodosthenous Cicek Topcu Christina Papa Antonia Aristokleous Georgia Stathi Christina Christodoulou Christina Eleftheriou Dora C. Stylianou Leondios G. Kostrikis
author_sort	Andreas C. Chrysostomou
title	A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
title_short	A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
title_full	A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
title_fullStr	A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
title_full_unstemmed	A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
title_sort	multiallelic molecular beacon-based real-time rt-pcr assay for the detection of sars-cov-2
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/41e2d50fe104486dac5120ed21c6bcf5
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A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2

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