Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea

Ticks are vectors that spread pathogenic bacteria, viruses, and protozoa. As the number of ticks increases due to climate change, the importance of the study of tick-borne pathogens has also increased. This study was conducted to investigate the distribution of the major tick species causing Lyme bo...

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Autores principales:	Haeseung Lee, Seung-Hun Lee, SungShik Shin, Dongmi Kwak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Borrelia</i> Korea phylogeny tick tick-borne pathogen Science Q
Acceso en línea:	https://doaj.org/article/41f61ed5271b4346b10cf98c040b5a19
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spelling	oai:doaj.org-article:41f61ed5271b4346b10cf98c040b5a192021-11-25T17:59:41ZMolecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea10.3390/insects121110112075-4450https://doaj.org/article/41f61ed5271b4346b10cf98c040b5a192021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/1011https://doaj.org/toc/2075-4450Ticks are vectors that spread pathogenic bacteria, viruses, and protozoa. As the number of ticks increases due to climate change, the importance of the study of tick-borne pathogens has also increased. This study was conducted to investigate the distribution of the major tick species causing Lyme borreliosis and regional differences in the prevalence of <i>Borrelia</i> spp. by tick species. <i>Borrelia</i> infection was confirmed not only in <i>Ixodes</i> ticks, which are the major vectors of <i>Borrelia</i> spp., but also in <i>Haemaphysalis</i> and <i>Amblyomma</i> ticks. PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl) was performed to confirm <i>Borrelia</i> positivity. A total of 6102 ticks (736 pools) were tested, and the proportion was <i>Haemaphysalis longicornis</i> nymphs and adults at 69.2%, <i>Haemaphysalis flava</i> nymphs and adults at 13.9%, <i>Haemaphysalis</i> spp. larva at 14.3%, <i>Ixodes nipponensis</i> at 0.8%, and <i>Amblyomma testudinarium</i> at 1.9%. <i>Ixodes nipponensis</i> showed the highest minimum infection rate (MIR: 34.00; 17 pools/50 ticks) for <i>Borrelia</i> spp., followed by <i>A. testudinarium</i> (MIR: 0.88), and <i>H. longicornis</i> (MIR: 0.05). In particular, to our knowledge <i>Borrelia</i> infection was first confirmed in <i>A. testudinarium</i> in Korea. As a result of phylogenetic analysis, all sequences were grouped with <i>B</i><i>orrelia</i><i>afzelii</i> isolates and showed a close relationship with high identity. Considering that <i>B. afzelii</i> causes infectious zoonotic diseases, continuous monitoring and attention are needed, although it has a low prevalence in this study.Haeseung LeeSeung-Hun LeeSungShik ShinDongmi KwakMDPI AGarticle<i>Borrelia</i>Koreaphylogenyticktick-borne pathogenScienceQENInsects, Vol 12, Iss 1011, p 1011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Borrelia</i> Korea phylogeny tick tick-borne pathogen Science Q
spellingShingle	<i>Borrelia</i> Korea phylogeny tick tick-borne pathogen Science Q Haeseung Lee Seung-Hun Lee SungShik Shin Dongmi Kwak Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
description	Ticks are vectors that spread pathogenic bacteria, viruses, and protozoa. As the number of ticks increases due to climate change, the importance of the study of tick-borne pathogens has also increased. This study was conducted to investigate the distribution of the major tick species causing Lyme borreliosis and regional differences in the prevalence of <i>Borrelia</i> spp. by tick species. <i>Borrelia</i> infection was confirmed not only in <i>Ixodes</i> ticks, which are the major vectors of <i>Borrelia</i> spp., but also in <i>Haemaphysalis</i> and <i>Amblyomma</i> ticks. PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl) was performed to confirm <i>Borrelia</i> positivity. A total of 6102 ticks (736 pools) were tested, and the proportion was <i>Haemaphysalis longicornis</i> nymphs and adults at 69.2%, <i>Haemaphysalis flava</i> nymphs and adults at 13.9%, <i>Haemaphysalis</i> spp. larva at 14.3%, <i>Ixodes nipponensis</i> at 0.8%, and <i>Amblyomma testudinarium</i> at 1.9%. <i>Ixodes nipponensis</i> showed the highest minimum infection rate (MIR: 34.00; 17 pools/50 ticks) for <i>Borrelia</i> spp., followed by <i>A. testudinarium</i> (MIR: 0.88), and <i>H. longicornis</i> (MIR: 0.05). In particular, to our knowledge <i>Borrelia</i> infection was first confirmed in <i>A. testudinarium</i> in Korea. As a result of phylogenetic analysis, all sequences were grouped with <i>B</i><i>orrelia</i><i>afzelii</i> isolates and showed a close relationship with high identity. Considering that <i>B. afzelii</i> causes infectious zoonotic diseases, continuous monitoring and attention are needed, although it has a low prevalence in this study.
format	article
author	Haeseung Lee Seung-Hun Lee SungShik Shin Dongmi Kwak
author_facet	Haeseung Lee Seung-Hun Lee SungShik Shin Dongmi Kwak
author_sort	Haeseung Lee
title	Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
title_short	Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
title_full	Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
title_fullStr	Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
title_full_unstemmed	Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea
title_sort	molecular identification of <i>borrelia</i> spp. from ticks in pastures nearby livestock farms in korea
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/41f61ed5271b4346b10cf98c040b5a19
work_keys_str_mv	AT haeseunglee molecularidentificationofiborreliaisppfromticksinpasturesnearbylivestockfarmsinkorea AT seunghunlee molecularidentificationofiborreliaisppfromticksinpasturesnearbylivestockfarmsinkorea AT sungshikshin molecularidentificationofiborreliaisppfromticksinpasturesnearbylivestockfarmsinkorea AT dongmikwak molecularidentificationofiborreliaisppfromticksinpasturesnearbylivestockfarmsinkorea
_version_	1718411738268827648

Molecular Identification of <i>Borrelia</i> spp. from Ticks in Pastures Nearby Livestock Farms in Korea

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