Single-cell states in the estrogen response of breast cancer cell lines.

Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for t...

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Autores principales:	Francesco Paolo Casale, Giorgio Giurato, Giovanni Nassa, Jonathan W Armond, Chris J Oates, Davide Corá, Andrea Gamba, Sach Mukherjee, Alessandro Weisz, Mario Nicodemi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/41f927f14c2b4216af613dacc620646e
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spelling	oai:doaj.org-article:41f927f14c2b4216af613dacc620646e2021-11-18T08:31:12ZSingle-cell states in the estrogen response of breast cancer cell lines.1932-620310.1371/journal.pone.0088485https://doaj.org/article/41f927f14c2b4216af613dacc620646e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586334/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified.Francesco Paolo CasaleGiorgio GiuratoGiovanni NassaJonathan W ArmondChris J OatesDavide CoráAndrea GambaSach MukherjeeAlessandro WeiszMario NicodemiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88485 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi Single-cell states in the estrogen response of breast cancer cell lines.
description	Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified.
format	article
author	Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi
author_facet	Francesco Paolo Casale Giorgio Giurato Giovanni Nassa Jonathan W Armond Chris J Oates Davide Corá Andrea Gamba Sach Mukherjee Alessandro Weisz Mario Nicodemi
author_sort	Francesco Paolo Casale
title	Single-cell states in the estrogen response of breast cancer cell lines.
title_short	Single-cell states in the estrogen response of breast cancer cell lines.
title_full	Single-cell states in the estrogen response of breast cancer cell lines.
title_fullStr	Single-cell states in the estrogen response of breast cancer cell lines.
title_full_unstemmed	Single-cell states in the estrogen response of breast cancer cell lines.
title_sort	single-cell states in the estrogen response of breast cancer cell lines.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/41f927f14c2b4216af613dacc620646e
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Single-cell states in the estrogen response of breast cancer cell lines.

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