<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication

ABSTRACT Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often complicated by secondary coinfection with bacterial pathogens such as Staphylococcus aureus. Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication in vitro in primary cell...

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Autores principales:	Mariya I. Goncheva, Carina Conceicao, Stephen W. Tuffs, Hui-Min Lee, Marlynne Quigg-Nicol, Ian Bennet, Fiona Sargison, Amy C. Pickering, Saira Hussain, Andrew C. Gill, Bernadette M. Dutia, Paul Digard, J. Ross Fitzgerald
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Staphylococcus aureus influenza influenza vaccines lipase pathogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/41ff10f22b9347849dc35a2197b537ad
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spelling	oai:doaj.org-article:41ff10f22b9347849dc35a2197b537ad2021-11-15T15:56:43Z<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication10.1128/mBio.00975-202150-7511https://doaj.org/article/41ff10f22b9347849dc35a2197b537ad2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00975-20https://doaj.org/toc/2150-7511ABSTRACT Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often complicated by secondary coinfection with bacterial pathogens such as Staphylococcus aureus. Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication in vitro in primary cells, including human lung fibroblasts. The proviral activity of lipase 1 is dependent on its enzymatic function, acts late in the viral life cycle, and results in increased infectivity through positive modulation of virus budding. Furthermore, the proviral effect of lipase 1 on IAV is exhibited during in vivo infection of embryonated hen’s eggs and, importantly, increases the yield of a vaccine strain of IAV by approximately 5-fold. Thus, we have identified the first S. aureus protein to enhance IAV replication, suggesting a potential role in coinfection. Importantly, this activity may be harnessed to address global shortages of influenza vaccines. IMPORTANCE Influenza A virus (IAV) causes annual epidemics and sporadic pandemics of respiratory disease. Secondary bacterial coinfection by organisms such as Staphylococcus aureus is the most common complication of primary IAV infection and is associated with high levels of morbidity and mortality. Here, we report the first identified S. aureus factor (lipase 1) that enhances IAV replication during infection via positive modulation of virus budding. The effect is observed in vivo in embryonated hen’s eggs and greatly enhances the yield of a vaccine strain, a finding that could be applied to address global shortages of influenza vaccines.Mariya I. GonchevaCarina ConceicaoStephen W. TuffsHui-Min LeeMarlynne Quigg-NicolIan BennetFiona SargisonAmy C. PickeringSaira HussainAndrew C. GillBernadette M. DutiaPaul DigardJ. Ross FitzgeraldAmerican Society for MicrobiologyarticleStaphylococcus aureusinfluenzainfluenza vaccineslipasepathogenesisMicrobiologyQR1-502ENmBio, Vol 11, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus aureus influenza influenza vaccines lipase pathogenesis Microbiology QR1-502
spellingShingle	Staphylococcus aureus influenza influenza vaccines lipase pathogenesis Microbiology QR1-502 Mariya I. Goncheva Carina Conceicao Stephen W. Tuffs Hui-Min Lee Marlynne Quigg-Nicol Ian Bennet Fiona Sargison Amy C. Pickering Saira Hussain Andrew C. Gill Bernadette M. Dutia Paul Digard J. Ross Fitzgerald <named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
description	ABSTRACT Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often complicated by secondary coinfection with bacterial pathogens such as Staphylococcus aureus. Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication in vitro in primary cells, including human lung fibroblasts. The proviral activity of lipase 1 is dependent on its enzymatic function, acts late in the viral life cycle, and results in increased infectivity through positive modulation of virus budding. Furthermore, the proviral effect of lipase 1 on IAV is exhibited during in vivo infection of embryonated hen’s eggs and, importantly, increases the yield of a vaccine strain of IAV by approximately 5-fold. Thus, we have identified the first S. aureus protein to enhance IAV replication, suggesting a potential role in coinfection. Importantly, this activity may be harnessed to address global shortages of influenza vaccines. IMPORTANCE Influenza A virus (IAV) causes annual epidemics and sporadic pandemics of respiratory disease. Secondary bacterial coinfection by organisms such as Staphylococcus aureus is the most common complication of primary IAV infection and is associated with high levels of morbidity and mortality. Here, we report the first identified S. aureus factor (lipase 1) that enhances IAV replication during infection via positive modulation of virus budding. The effect is observed in vivo in embryonated hen’s eggs and greatly enhances the yield of a vaccine strain, a finding that could be applied to address global shortages of influenza vaccines.
format	article
author	Mariya I. Goncheva Carina Conceicao Stephen W. Tuffs Hui-Min Lee Marlynne Quigg-Nicol Ian Bennet Fiona Sargison Amy C. Pickering Saira Hussain Andrew C. Gill Bernadette M. Dutia Paul Digard J. Ross Fitzgerald
author_facet	Mariya I. Goncheva Carina Conceicao Stephen W. Tuffs Hui-Min Lee Marlynne Quigg-Nicol Ian Bennet Fiona Sargison Amy C. Pickering Saira Hussain Andrew C. Gill Bernadette M. Dutia Paul Digard J. Ross Fitzgerald
author_sort	Mariya I. Goncheva
title	<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
title_short	<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
title_full	<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
title_fullStr	<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
title_full_unstemmed	<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication
title_sort	<named-content content-type="genus-species">staphylococcus aureus</named-content> lipase 1 enhances influenza a virus replication
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/41ff10f22b9347849dc35a2197b537ad
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<named-content content-type="genus-species">Staphylococcus aureus</named-content> Lipase 1 Enhances Influenza A Virus Replication

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