Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing...
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MDPI AG
2021
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oai:doaj.org-article:42003c78f96b4b47aead90822d9a2e212021-11-25T18:37:44ZRetrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh10.3390/pathogens101113732076-0817https://doaj.org/article/42003c78f96b4b47aead90822d9a2e212021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1373https://doaj.org/toc/2076-0817Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.Tofazzal Md RakibBabu Kanti NathTridip DasSaroj Kumar YadavShane R. RaidalShubhagata DasMDPI AGarticlecanine parvoviruswhole genome shotgun sequencingPCR-HRMnew CPV-2aMedicineRENPathogens, Vol 10, Iss 1373, p 1373 (2021) |
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canine parvovirus whole genome shotgun sequencing PCR-HRM new CPV-2a Medicine R |
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canine parvovirus whole genome shotgun sequencing PCR-HRM new CPV-2a Medicine R Tofazzal Md Rakib Babu Kanti Nath Tridip Das Saroj Kumar Yadav Shane R. Raidal Shubhagata Das Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
description |
Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh. |
format |
article |
author |
Tofazzal Md Rakib Babu Kanti Nath Tridip Das Saroj Kumar Yadav Shane R. Raidal Shubhagata Das |
author_facet |
Tofazzal Md Rakib Babu Kanti Nath Tridip Das Saroj Kumar Yadav Shane R. Raidal Shubhagata Das |
author_sort |
Tofazzal Md Rakib |
title |
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
title_short |
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
title_full |
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
title_fullStr |
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
title_full_unstemmed |
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh |
title_sort |
retrospective genotyping and whole genome sequencing of a canine parvovirus outbreak in bangladesh |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/42003c78f96b4b47aead90822d9a2e21 |
work_keys_str_mv |
AT tofazzalmdrakib retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh AT babukantinath retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh AT tridipdas retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh AT sarojkumaryadav retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh AT shanerraidal retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh AT shubhagatadas retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh |
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1718410888302559232 |