Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh

Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing...

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Autores principales: Tofazzal Md Rakib, Babu Kanti Nath, Tridip Das, Saroj Kumar Yadav, Shane R. Raidal, Shubhagata Das
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Lenguaje:EN
Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/42003c78f96b4b47aead90822d9a2e21
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spelling oai:doaj.org-article:42003c78f96b4b47aead90822d9a2e212021-11-25T18:37:44ZRetrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh10.3390/pathogens101113732076-0817https://doaj.org/article/42003c78f96b4b47aead90822d9a2e212021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1373https://doaj.org/toc/2076-0817Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.Tofazzal Md RakibBabu Kanti NathTridip DasSaroj Kumar YadavShane R. RaidalShubhagata DasMDPI AGarticlecanine parvoviruswhole genome shotgun sequencingPCR-HRMnew CPV-2aMedicineRENPathogens, Vol 10, Iss 1373, p 1373 (2021)
institution DOAJ
collection DOAJ
language EN
topic canine parvovirus
whole genome shotgun sequencing
PCR-HRM
new CPV-2a
Medicine
R
spellingShingle canine parvovirus
whole genome shotgun sequencing
PCR-HRM
new CPV-2a
Medicine
R
Tofazzal Md Rakib
Babu Kanti Nath
Tridip Das
Saroj Kumar Yadav
Shane R. Raidal
Shubhagata Das
Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
description Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.
format article
author Tofazzal Md Rakib
Babu Kanti Nath
Tridip Das
Saroj Kumar Yadav
Shane R. Raidal
Shubhagata Das
author_facet Tofazzal Md Rakib
Babu Kanti Nath
Tridip Das
Saroj Kumar Yadav
Shane R. Raidal
Shubhagata Das
author_sort Tofazzal Md Rakib
title Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
title_short Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
title_full Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
title_fullStr Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
title_full_unstemmed Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh
title_sort retrospective genotyping and whole genome sequencing of a canine parvovirus outbreak in bangladesh
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/42003c78f96b4b47aead90822d9a2e21
work_keys_str_mv AT tofazzalmdrakib retrospectivegenotypingandwholegenomesequencingofacanineparvovirusoutbreakinbangladesh
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