Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.

Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium. To develop transgenesis as a functional approach for these pathogens, we previously demonstrated that pseu...

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Autores principales:	Gabriel Rinaldi, Sabine E Eckert, Isheng J Tsai, Sutas Suttiprapa, Kristine J Kines, José F Tort, Victoria H Mann, Daniel J Turner, Matthew Berriman, Paul J Brindley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/420675da718e475b97585bd56cfbe2f5
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spelling	oai:doaj.org-article:420675da718e475b97585bd56cfbe2f52021-11-18T06:04:10ZGermline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.1553-73661553-737410.1371/journal.ppat.1002820https://doaj.org/article/420675da718e475b97585bd56cfbe2f52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22911241/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium. To develop transgenesis as a functional approach for these pathogens, we previously demonstrated that pseudotyped murine leukemia virus (MLV) can transduce schistosomes leading to chromosomal integration of reporter transgenes and short hairpin RNA cassettes. Here we investigated vertical transmission of transgenes through the developmental cycle of S. mansoni after introducing transgenes into eggs. Although MLV infection of schistosome eggs from mouse livers was efficient in terms of snail infectivity, >10-fold higher transgene copy numbers were detected in cercariae derived from in vitro laid eggs (IVLE). After infecting snails with miracidia from eggs transduced by MLV, sequencing of genomic DNA from cercariae released from the snails also revealed the presence of transgenes, demonstrating that transgenes had been transmitted through the asexual developmental cycle, and thereby confirming germline transgenesis. High-throughput sequencing of genomic DNA from schistosome populations exposed to MLV mapped widespread and random insertion of transgenes throughout the genome, along each of the autosomes and sex chromosomes, validating the utility of this approach for insertional mutagenesis. In addition, the germline-transmitted transgene encoding neomycin phosphotransferase rescued cultured schistosomules from toxicity of the antibiotic G418, and PCR analysis of eggs resulting from sexual reproduction of the transgenic worms in mice confirmed that retroviral transgenes were transmitted to the next (F1) generation. These findings provide the first description of wide-scale, random insertional mutagenesis of chromosomes and of germline transmission of a transgene in schistosomes. Transgenic lines of schistosomes expressing antibiotic resistance could advance functional genomics for these significant human pathogens. DATABASE ACCESSION: Sequence data from this study have been submitted to the European Nucleotide Archive (http://www.ebi.ac.uk/embl) under accession number ERP000379.Gabriel RinaldiSabine E EckertIsheng J TsaiSutas SuttiprapaKristine J KinesJosé F TortVictoria H MannDaniel J TurnerMatthew BerrimanPaul J BrindleyPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 7, p e1002820 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Gabriel Rinaldi Sabine E Eckert Isheng J Tsai Sutas Suttiprapa Kristine J Kines José F Tort Victoria H Mann Daniel J Turner Matthew Berriman Paul J Brindley Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
description	Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium. To develop transgenesis as a functional approach for these pathogens, we previously demonstrated that pseudotyped murine leukemia virus (MLV) can transduce schistosomes leading to chromosomal integration of reporter transgenes and short hairpin RNA cassettes. Here we investigated vertical transmission of transgenes through the developmental cycle of S. mansoni after introducing transgenes into eggs. Although MLV infection of schistosome eggs from mouse livers was efficient in terms of snail infectivity, >10-fold higher transgene copy numbers were detected in cercariae derived from in vitro laid eggs (IVLE). After infecting snails with miracidia from eggs transduced by MLV, sequencing of genomic DNA from cercariae released from the snails also revealed the presence of transgenes, demonstrating that transgenes had been transmitted through the asexual developmental cycle, and thereby confirming germline transgenesis. High-throughput sequencing of genomic DNA from schistosome populations exposed to MLV mapped widespread and random insertion of transgenes throughout the genome, along each of the autosomes and sex chromosomes, validating the utility of this approach for insertional mutagenesis. In addition, the germline-transmitted transgene encoding neomycin phosphotransferase rescued cultured schistosomules from toxicity of the antibiotic G418, and PCR analysis of eggs resulting from sexual reproduction of the transgenic worms in mice confirmed that retroviral transgenes were transmitted to the next (F1) generation. These findings provide the first description of wide-scale, random insertional mutagenesis of chromosomes and of germline transmission of a transgene in schistosomes. Transgenic lines of schistosomes expressing antibiotic resistance could advance functional genomics for these significant human pathogens. DATABASE ACCESSION: Sequence data from this study have been submitted to the European Nucleotide Archive (http://www.ebi.ac.uk/embl) under accession number ERP000379.
format	article
author	Gabriel Rinaldi Sabine E Eckert Isheng J Tsai Sutas Suttiprapa Kristine J Kines José F Tort Victoria H Mann Daniel J Turner Matthew Berriman Paul J Brindley
author_facet	Gabriel Rinaldi Sabine E Eckert Isheng J Tsai Sutas Suttiprapa Kristine J Kines José F Tort Victoria H Mann Daniel J Turner Matthew Berriman Paul J Brindley
author_sort	Gabriel Rinaldi
title	Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
title_short	Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
title_full	Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
title_fullStr	Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
title_full_unstemmed	Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
title_sort	germline transgenesis and insertional mutagenesis in schistosoma mansoni mediated by murine leukemia virus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/420675da718e475b97585bd56cfbe2f5
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Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.

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