BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.

A novel function for the binder of Arl two (BART) molecule in pancreatic cancer cells is reported. BART inhibits invasiveness of pancreatic cancer cells through binding to a Ca(2+)-dependent, phosphorylated, guanosine triphosphatase (GTPase) membrane fusion protein, annexin7 (ANX7). A tumor suppress...

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Autores principales:	Keisuke Taniuchi, Kunihiko Yokotani, Toshiji Saibara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/420a29af32d44633811de783f5d3c27b
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spelling	oai:doaj.org-article:420a29af32d44633811de783f5d3c27b2021-11-18T07:21:40ZBART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.1932-620310.1371/journal.pone.0035674https://doaj.org/article/420a29af32d44633811de783f5d3c27b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22532868/?tool=EBIhttps://doaj.org/toc/1932-6203A novel function for the binder of Arl two (BART) molecule in pancreatic cancer cells is reported. BART inhibits invasiveness of pancreatic cancer cells through binding to a Ca(2+)-dependent, phosphorylated, guanosine triphosphatase (GTPase) membrane fusion protein, annexin7 (ANX7). A tumor suppressor function for ANX7 was previously reported based on its prognostic role in human cancers and the cancer-prone mouse phenotype ANX7(+/-). Further investigation demonstrated that the BART-ANX7 complex is transported toward cell protrusions in migrating cells when BART supports the binding of ANX7 to the protein kinase C (PKC) isoform PKCα. Recent evidence has suggested that phosphorylation of ANX7 by PKC significantly potentiates ANX7-induced fusion of phospholipid vesicles; however, the current data suggest that the BART-ANX7 complex reduces PKCα activity. Knocking down endogenous BART and ANX7 increases activity of PKCα, and specific inhibitors of PKCα significantly abrogate invasiveness induced by BART and ANX7 knockdown. These results imply that BART contributes to regulating PKCα activity through binding to ANX7, thereby affecting the invasiveness of pancreatic cancer cells. Thus, it is possible that BART and ANX7 can distinctly regulate the downstream signaling of PKCα that is potentially relevant to cell invasion by acting as anti-invasive molecules.Keisuke TaniuchiKunihiko YokotaniToshiji SaibaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35674 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keisuke Taniuchi Kunihiko Yokotani Toshiji Saibara BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
description	A novel function for the binder of Arl two (BART) molecule in pancreatic cancer cells is reported. BART inhibits invasiveness of pancreatic cancer cells through binding to a Ca(2+)-dependent, phosphorylated, guanosine triphosphatase (GTPase) membrane fusion protein, annexin7 (ANX7). A tumor suppressor function for ANX7 was previously reported based on its prognostic role in human cancers and the cancer-prone mouse phenotype ANX7(+/-). Further investigation demonstrated that the BART-ANX7 complex is transported toward cell protrusions in migrating cells when BART supports the binding of ANX7 to the protein kinase C (PKC) isoform PKCα. Recent evidence has suggested that phosphorylation of ANX7 by PKC significantly potentiates ANX7-induced fusion of phospholipid vesicles; however, the current data suggest that the BART-ANX7 complex reduces PKCα activity. Knocking down endogenous BART and ANX7 increases activity of PKCα, and specific inhibitors of PKCα significantly abrogate invasiveness induced by BART and ANX7 knockdown. These results imply that BART contributes to regulating PKCα activity through binding to ANX7, thereby affecting the invasiveness of pancreatic cancer cells. Thus, it is possible that BART and ANX7 can distinctly regulate the downstream signaling of PKCα that is potentially relevant to cell invasion by acting as anti-invasive molecules.
format	article
author	Keisuke Taniuchi Kunihiko Yokotani Toshiji Saibara
author_facet	Keisuke Taniuchi Kunihiko Yokotani Toshiji Saibara
author_sort	Keisuke Taniuchi
title	BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
title_short	BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
title_full	BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
title_fullStr	BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
title_full_unstemmed	BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
title_sort	bart inhibits pancreatic cancer cell invasion by pkcα inactivation through binding to anx7.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/420a29af32d44633811de783f5d3c27b
work_keys_str_mv	AT keisuketaniuchi bartinhibitspancreaticcancercellinvasionbypkcainactivationthroughbindingtoanx7 AT kunihikoyokotani bartinhibitspancreaticcancercellinvasionbypkcainactivationthroughbindingtoanx7 AT toshijisaibara bartinhibitspancreaticcancercellinvasionbypkcainactivationthroughbindingtoanx7
_version_	1718423592224423936

BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.

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