In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type

Abstract DNA methylation plays an important role in modulating plant growth plasticity in response to stress, but mechanisms involved in such control need further investigation. We used drm1 drm2 cmt3 mutant of Arabidopsis thaliana, defective in DNA methylation, to explore metabolic pathways downstr...

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Autores principales:	Marianna Pacenza, Antonella Muto, Adriana Chiappetta, Lorenzo Mariotti, Emanuela Talarico, Piero Picciarelli, Ernesto Picardi, Leonardo Bruno, Maria Beatrice Bitonti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/420c726489c44d36a89d72e63ba031c3
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spelling	oai:doaj.org-article:420c726489c44d36a89d72e63ba031c32021-12-02T14:49:17ZIn Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type10.1038/s41598-021-90528-52045-2322https://doaj.org/article/420c726489c44d36a89d72e63ba031c32021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90528-5https://doaj.org/toc/2045-2322Abstract DNA methylation plays an important role in modulating plant growth plasticity in response to stress, but mechanisms involved in such control need further investigation. We used drm1 drm2 cmt3 mutant of Arabidopsis thaliana, defective in DNA methylation, to explore metabolic pathways downstream epigenetic modulation under cadmium (Cd) stress. To this aim, a transcriptomic analysis was performed on ddc and WT plants exposed to a long-lasting (21 d) Cd treatment (25/50 µM), focusing on hormone genetic pathways. Growth parameters and hormones amount were also estimated. Transcriptomic data and hormone quantification showed that, under prolonged Cd treatment, level and signalling of growth-sustaining hormones (auxins, CKs, GAs) were enhanced and/or maintained, while a decrease was detected for stress-related hormones (JA, ABA, SA), likely as a strategy to avoid the side effects of their long-lasting activation. Such picture was more effective in ddc than WT, already at 25 µM Cd, in line with its better growth performance. A tight relationship between methylation status and the modulation of hormone genetic pathways under Cd stress was assessed. We propose that the higher genome plasticity conferred to ddc by DNA hypomethylated status underlies its prompt response to modulate hormones genetic pathways and activity and assure a flexible growth.Marianna PacenzaAntonella MutoAdriana ChiappettaLorenzo MariottiEmanuela TalaricoPiero PicciarelliErnesto PicardiLeonardo BrunoMaria Beatrice BitontiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marianna Pacenza Antonella Muto Adriana Chiappetta Lorenzo Mariotti Emanuela Talarico Piero Picciarelli Ernesto Picardi Leonardo Bruno Maria Beatrice Bitonti In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
description	Abstract DNA methylation plays an important role in modulating plant growth plasticity in response to stress, but mechanisms involved in such control need further investigation. We used drm1 drm2 cmt3 mutant of Arabidopsis thaliana, defective in DNA methylation, to explore metabolic pathways downstream epigenetic modulation under cadmium (Cd) stress. To this aim, a transcriptomic analysis was performed on ddc and WT plants exposed to a long-lasting (21 d) Cd treatment (25/50 µM), focusing on hormone genetic pathways. Growth parameters and hormones amount were also estimated. Transcriptomic data and hormone quantification showed that, under prolonged Cd treatment, level and signalling of growth-sustaining hormones (auxins, CKs, GAs) were enhanced and/or maintained, while a decrease was detected for stress-related hormones (JA, ABA, SA), likely as a strategy to avoid the side effects of their long-lasting activation. Such picture was more effective in ddc than WT, already at 25 µM Cd, in line with its better growth performance. A tight relationship between methylation status and the modulation of hormone genetic pathways under Cd stress was assessed. We propose that the higher genome plasticity conferred to ddc by DNA hypomethylated status underlies its prompt response to modulate hormones genetic pathways and activity and assure a flexible growth.
format	article
author	Marianna Pacenza Antonella Muto Adriana Chiappetta Lorenzo Mariotti Emanuela Talarico Piero Picciarelli Ernesto Picardi Leonardo Bruno Maria Beatrice Bitonti
author_facet	Marianna Pacenza Antonella Muto Adriana Chiappetta Lorenzo Mariotti Emanuela Talarico Piero Picciarelli Ernesto Picardi Leonardo Bruno Maria Beatrice Bitonti
author_sort	Marianna Pacenza
title	In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
title_short	In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
title_full	In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
title_fullStr	In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
title_full_unstemmed	In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
title_sort	in arabidopsis thaliana cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/420c726489c44d36a89d72e63ba031c3
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In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type

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