Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in <i>Escherichia coli</i> (<i>E. coli</i>), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed t...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/420ef79ae40f426fbc036546c1fc893b |
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| Sumario: | Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in <i>Escherichia coli</i> (<i>E. coli</i>), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed to characterise the genetic features of ESC-resistant <i>E. coli</i> isolated from cats and dogs with urinary tract infections in Thailand. Of 72 ESC-resistant <i>E. coli</i> isolated from diagnostic samples (2016–2018), <i>bla</i><sub>CTX-M</sub> including group 1 (CTX-M-55, -15 and -173) and group 9 (CTX-M-14, -27, -65 and -90) variants were detected in 47 isolates (65.28%) using PCR and DNA sequencing. Additional antimicrobial resistance genes, including plasmid-mediated AmpC (CIT and DHA), <i>bla</i><sub>NDM-5</sub>, <i>mcr-3</i>, <i>mph</i>(A) and <i>aac(6′)-Ib-cr</i>, were detected in these isolates. Using a broth microdilution assay, all the strains exhibited multidrug-resistant phenotypes. The phylogroups were F (36.11%), A (20.83%), B1 (19.44%), B2 (19.44%) and D (4.17%), with several virulence genes, plasmid replicons and an integrase gene. The DNA fingerprinting using a repetitive extragenic palindromic sequence-PCR presented clonal relationships within phylogroups. Multiple human-associated, high-risk ExPEC clones associated with multidrug resistance, including sequence type (ST) 38, ST131, ST224, ST167, ST354, ST410, ST617 and ST648, were identified, suggesting clonal dissemination. Dogs and cats are a potential reservoir of ESC-resistant <i>E. coli</i> and significant antimicrobial resistance genes. |
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