Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand

Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand

Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in <i>Escherichia coli</i> (<i>E. coli</i>), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed t...

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Autores principales: Naiyaphat Nittayasut, Jitrapa Yindee, Pongthai Boonkham, Teerapong Yata, Nipattra Suanpairintr, Pattrarat Chanchaithong
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>bla</i><sub>CTX-M</sub>
carbapenemase
pet
clones
uropathogenic <i>Escherichia coli</i>
Therapeutics. Pharmacology
RM1-950
Acceso en línea:https://doaj.org/article/420ef79ae40f426fbc036546c1fc893b
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spelling oai:doaj.org-article:420ef79ae40f426fbc036546c1fc893b2021-11-25T16:24:11ZMultiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand10.3390/antibiotics101113742079-6382https://doaj.org/article/420ef79ae40f426fbc036546c1fc893b2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1374https://doaj.org/toc/2079-6382Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in <i>Escherichia coli</i> (<i>E. coli</i>), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed to characterise the genetic features of ESC-resistant <i>E. coli</i> isolated from cats and dogs with urinary tract infections in Thailand. Of 72 ESC-resistant <i>E. coli</i> isolated from diagnostic samples (2016–2018), <i>bla</i><sub>CTX-M</sub> including group 1 (CTX-M-55, -15 and -173) and group 9 (CTX-M-14, -27, -65 and -90) variants were detected in 47 isolates (65.28%) using PCR and DNA sequencing. Additional antimicrobial resistance genes, including plasmid-mediated AmpC (CIT and DHA), <i>bla</i><sub>NDM-5</sub>, <i>mcr-3</i>, <i>mph</i>(A) and <i>aac(6′)-Ib-cr</i>, were detected in these isolates. Using a broth microdilution assay, all the strains exhibited multidrug-resistant phenotypes. The phylogroups were F (36.11%), A (20.83%), B1 (19.44%), B2 (19.44%) and D (4.17%), with several virulence genes, plasmid replicons and an integrase gene. The DNA fingerprinting using a repetitive extragenic palindromic sequence-PCR presented clonal relationships within phylogroups. Multiple human-associated, high-risk ExPEC clones associated with multidrug resistance, including sequence type (ST) 38, ST131, ST224, ST167, ST354, ST410, ST617 and ST648, were identified, suggesting clonal dissemination. Dogs and cats are a potential reservoir of ESC-resistant <i>E. coli</i> and significant antimicrobial resistance genes.Naiyaphat NittayasutJitrapa YindeePongthai BoonkhamTeerapong YataNipattra SuanpairintrPattrarat ChanchaithongMDPI AGarticle<i>bla</i><sub>CTX-M</sub>carbapenemasepetclonesuropathogenic <i>Escherichia coli</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1374, p 1374 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>bla</i><sub>CTX-M</sub>
carbapenemase
pet
clones
uropathogenic <i>Escherichia coli</i>
Therapeutics. Pharmacology
RM1-950
spellingShingle <i>bla</i><sub>CTX-M</sub>
carbapenemase
pet
clones
uropathogenic <i>Escherichia coli</i>
Therapeutics. Pharmacology
RM1-950
Naiyaphat Nittayasut
Jitrapa Yindee
Pongthai Boonkham
Teerapong Yata
Nipattra Suanpairintr
Pattrarat Chanchaithong
Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
description Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in <i>Escherichia coli</i> (<i>E. coli</i>), increasingly identified in small animals, indicates a crisis of an antimicrobial resistance situation in veterinary medicine and public health. This study aimed to characterise the genetic features of ESC-resistant <i>E. coli</i> isolated from cats and dogs with urinary tract infections in Thailand. Of 72 ESC-resistant <i>E. coli</i> isolated from diagnostic samples (2016–2018), <i>bla</i><sub>CTX-M</sub> including group 1 (CTX-M-55, -15 and -173) and group 9 (CTX-M-14, -27, -65 and -90) variants were detected in 47 isolates (65.28%) using PCR and DNA sequencing. Additional antimicrobial resistance genes, including plasmid-mediated AmpC (CIT and DHA), <i>bla</i><sub>NDM-5</sub>, <i>mcr-3</i>, <i>mph</i>(A) and <i>aac(6′)-Ib-cr</i>, were detected in these isolates. Using a broth microdilution assay, all the strains exhibited multidrug-resistant phenotypes. The phylogroups were F (36.11%), A (20.83%), B1 (19.44%), B2 (19.44%) and D (4.17%), with several virulence genes, plasmid replicons and an integrase gene. The DNA fingerprinting using a repetitive extragenic palindromic sequence-PCR presented clonal relationships within phylogroups. Multiple human-associated, high-risk ExPEC clones associated with multidrug resistance, including sequence type (ST) 38, ST131, ST224, ST167, ST354, ST410, ST617 and ST648, were identified, suggesting clonal dissemination. Dogs and cats are a potential reservoir of ESC-resistant <i>E. coli</i> and significant antimicrobial resistance genes.
format article
author Naiyaphat Nittayasut
Jitrapa Yindee
Pongthai Boonkham
Teerapong Yata
Nipattra Suanpairintr
Pattrarat Chanchaithong
author_facet Naiyaphat Nittayasut
Jitrapa Yindee
Pongthai Boonkham
Teerapong Yata
Nipattra Suanpairintr
Pattrarat Chanchaithong
author_sort Naiyaphat Nittayasut
title Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
title_short Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
title_full Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
title_fullStr Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
title_full_unstemmed Multiple and High-Risk Clones of Extended-Spectrum Cephalosporin-Resistant and <i>bla</i><sub>NDM-5</sub>-Harbouring Uropathogenic <i>Escherichia coli</i> from Cats and Dogs in Thailand
title_sort multiple and high-risk clones of extended-spectrum cephalosporin-resistant and <i>bla</i><sub>ndm-5</sub>-harbouring uropathogenic <i>escherichia coli</i> from cats and dogs in thailand
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/420ef79ae40f426fbc036546c1fc893b
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