RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms

Abstract Intracranial aneurysm (IA) is a socially important disease as a major cause of subarachnoid hemorrhage. Recent experimental studies mainly using animal models have revealed a crucial role of macrophage-mediated chronic inflammatory responses in its pathogenesis. However, as findings from co...

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Autores principales:	Tomohiro Aoki, Hirokazu Koseki, Haruka Miyata, Masayoshi Itoh, Hideya Kawaji, Katsumi Takizawa, Akitsugu Kawashima, Hiroshi Ujiie, Takashi Higa, Kenzo Minamimura, Toshikazu Kimura, Hidetoshi Kasuya, Kazuhiko Nozaki, Akio Morita, Hirotoshi Sano, Shuh Narumiya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42132f10b4824a308c245646ef9a229d
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spelling	oai:doaj.org-article:42132f10b4824a308c245646ef9a229d2021-12-02T15:09:20ZRNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms10.1038/s41598-019-46886-22045-2322https://doaj.org/article/42132f10b4824a308c245646ef9a229d2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46886-2https://doaj.org/toc/2045-2322Abstract Intracranial aneurysm (IA) is a socially important disease as a major cause of subarachnoid hemorrhage. Recent experimental studies mainly using animal models have revealed a crucial role of macrophage-mediated chronic inflammatory responses in its pathogenesis. However, as findings from comprehensive analysis of unruptured human IAs are limited, factors regulating progression and rupture of IAs in humans remain unclear. Using surgically dissected human unruptured IA lesions and control arterial walls, gene expression profiles were obtained by RNA sequence analysis. RNA sequencing analysis was done with read count about 60~100 million which yielded 6~10 billion bases per sample. 79 over-expressed and 329 under-expressed genes in IA lesions were identified. Through Gene Ontology analysis, ‘chemokine activity’, ‘defense response’ and ‘extracellular region’ were picked up as over-represented terms which included CCL3 and CCL4 in common. Among these genes, quantitative RT-PCR analysis using another set of samples reproduced the above result. Finally, increase of CCL3 protein compared with that in control arterial walls was clarified in IA lesions. Findings of the present study again highlight importance of macrophage recruitment via CCL3 in the pathogenesis of IA progression.Tomohiro AokiHirokazu KosekiHaruka MiyataMasayoshi ItohHideya KawajiKatsumi TakizawaAkitsugu KawashimaHiroshi UjiieTakashi HigaKenzo MinamimuraToshikazu KimuraHidetoshi KasuyaKazuhiko NozakiAkio MoritaHirotoshi SanoShuh NarumiyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomohiro Aoki Hirokazu Koseki Haruka Miyata Masayoshi Itoh Hideya Kawaji Katsumi Takizawa Akitsugu Kawashima Hiroshi Ujiie Takashi Higa Kenzo Minamimura Toshikazu Kimura Hidetoshi Kasuya Kazuhiko Nozaki Akio Morita Hirotoshi Sano Shuh Narumiya RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
description	Abstract Intracranial aneurysm (IA) is a socially important disease as a major cause of subarachnoid hemorrhage. Recent experimental studies mainly using animal models have revealed a crucial role of macrophage-mediated chronic inflammatory responses in its pathogenesis. However, as findings from comprehensive analysis of unruptured human IAs are limited, factors regulating progression and rupture of IAs in humans remain unclear. Using surgically dissected human unruptured IA lesions and control arterial walls, gene expression profiles were obtained by RNA sequence analysis. RNA sequencing analysis was done with read count about 60~100 million which yielded 6~10 billion bases per sample. 79 over-expressed and 329 under-expressed genes in IA lesions were identified. Through Gene Ontology analysis, ‘chemokine activity’, ‘defense response’ and ‘extracellular region’ were picked up as over-represented terms which included CCL3 and CCL4 in common. Among these genes, quantitative RT-PCR analysis using another set of samples reproduced the above result. Finally, increase of CCL3 protein compared with that in control arterial walls was clarified in IA lesions. Findings of the present study again highlight importance of macrophage recruitment via CCL3 in the pathogenesis of IA progression.
format	article
author	Tomohiro Aoki Hirokazu Koseki Haruka Miyata Masayoshi Itoh Hideya Kawaji Katsumi Takizawa Akitsugu Kawashima Hiroshi Ujiie Takashi Higa Kenzo Minamimura Toshikazu Kimura Hidetoshi Kasuya Kazuhiko Nozaki Akio Morita Hirotoshi Sano Shuh Narumiya
author_facet	Tomohiro Aoki Hirokazu Koseki Haruka Miyata Masayoshi Itoh Hideya Kawaji Katsumi Takizawa Akitsugu Kawashima Hiroshi Ujiie Takashi Higa Kenzo Minamimura Toshikazu Kimura Hidetoshi Kasuya Kazuhiko Nozaki Akio Morita Hirotoshi Sano Shuh Narumiya
author_sort	Tomohiro Aoki
title	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
title_short	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
title_full	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
title_fullStr	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
title_full_unstemmed	RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms
title_sort	rna sequencing analysis revealed the induction of ccl3 expression in human intracranial aneurysms
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/42132f10b4824a308c245646ef9a229d
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RNA sequencing analysis revealed the induction of CCL3 expression in human intracranial aneurysms

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