Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.

Biomedical applications of carbon nanotubes (CNTs) often involve improving their hydrophilicity and dispersion in biological media by modifying them through noncovalent or covalent functionalization. However, the potential adverse effects of surface-functionalized CNTs have not been well characteriz...

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Autores principales:	Yuanqin Jiang, Honggang Zhang, Yange Wang, Min Chen, Shefang Ye, Zhenqing Hou, Lei Ren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4214e447a0774a7a91250f8c87af9cb2
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spelling	oai:doaj.org-article:4214e447a0774a7a91250f8c87af9cb22021-11-18T07:42:52ZModulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.1932-620310.1371/journal.pone.0065756https://doaj.org/article/4214e447a0774a7a91250f8c87af9cb22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755279/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Biomedical applications of carbon nanotubes (CNTs) often involve improving their hydrophilicity and dispersion in biological media by modifying them through noncovalent or covalent functionalization. However, the potential adverse effects of surface-functionalized CNTs have not been well characterized. In this study, we functionalized multi-walled CNTs (MWCNTs) via carboxylation, to produce MWCNTs-COOH, and via poly (ethylene glycol) linking, to produce MWCNTs-PEG. We used these functionalized MWCNTs to study the effect of surface functionalization on MWCNTs-induced toxicity to macrophages, and elucidate the underlying mechanisms of action. Our results revealed that MWCNTs-PEG were less cytotoxic and were associated with less apoptotic cell death of macrophages than MWCNTs-COOH. Additionally, MWCNTs-PEG induced less generation of reactive oxygen species (ROS) involving less activation of NADPH oxidase compared with MWCNTs-COOH, as evidenced by membrane translocation of p47(phox) and p67(phox) in macrophages. The less cytotoxic and apoptotic effect of MWCNTs-PEG compared with MWCNTs-COOH resulted from the lower cellular uptake of MWCNTs-PEG, which resulted in less activation of oxidative stress-responsive pathways, such as p38 mitogen-activated protein kinases (MAPK) and nuclear factor (NF)-κB. These results demonstrate that surface functionalization of CNTs may alter ROS-mediated cytotoxic and apoptotic response by modulating apoptotic signaling pathways. Our study thus provides new insights into the molecular basis for the surface properties affecting CNTs toxicity.Yuanqin JiangHonggang ZhangYange WangMin ChenShefang YeZhenqing HouLei RenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65756 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuanqin Jiang Honggang Zhang Yange Wang Min Chen Shefang Ye Zhenqing Hou Lei Ren Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
description	Biomedical applications of carbon nanotubes (CNTs) often involve improving their hydrophilicity and dispersion in biological media by modifying them through noncovalent or covalent functionalization. However, the potential adverse effects of surface-functionalized CNTs have not been well characterized. In this study, we functionalized multi-walled CNTs (MWCNTs) via carboxylation, to produce MWCNTs-COOH, and via poly (ethylene glycol) linking, to produce MWCNTs-PEG. We used these functionalized MWCNTs to study the effect of surface functionalization on MWCNTs-induced toxicity to macrophages, and elucidate the underlying mechanisms of action. Our results revealed that MWCNTs-PEG were less cytotoxic and were associated with less apoptotic cell death of macrophages than MWCNTs-COOH. Additionally, MWCNTs-PEG induced less generation of reactive oxygen species (ROS) involving less activation of NADPH oxidase compared with MWCNTs-COOH, as evidenced by membrane translocation of p47(phox) and p67(phox) in macrophages. The less cytotoxic and apoptotic effect of MWCNTs-PEG compared with MWCNTs-COOH resulted from the lower cellular uptake of MWCNTs-PEG, which resulted in less activation of oxidative stress-responsive pathways, such as p38 mitogen-activated protein kinases (MAPK) and nuclear factor (NF)-κB. These results demonstrate that surface functionalization of CNTs may alter ROS-mediated cytotoxic and apoptotic response by modulating apoptotic signaling pathways. Our study thus provides new insights into the molecular basis for the surface properties affecting CNTs toxicity.
format	article
author	Yuanqin Jiang Honggang Zhang Yange Wang Min Chen Shefang Ye Zhenqing Hou Lei Ren
author_facet	Yuanqin Jiang Honggang Zhang Yange Wang Min Chen Shefang Ye Zhenqing Hou Lei Ren
author_sort	Yuanqin Jiang
title	Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
title_short	Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
title_full	Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
title_fullStr	Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
title_full_unstemmed	Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
title_sort	modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4214e447a0774a7a91250f8c87af9cb2
work_keys_str_mv	AT yuanqinjiang modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT honggangzhang modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT yangewang modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT minchen modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT shefangye modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT zhenqinghou modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes AT leiren modulationofapoptoticpathwaysofmacrophagesbysurfacefunctionalizedmultiwalledcarbonnanotubes
_version_	1718423031188029440

Modulation of apoptotic pathways of macrophages by surface-functionalized multi-walled carbon nanotubes.

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