Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA

Abstract Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (sa...

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Autores principales:	Riham F. Hussein, Sherif Mohamed El Mahdy, Nashwa Mohammed Saged, L. Rashed, Sherien S. Ghaleb
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Real-time PCR multiplex Nasal secretions Saliva Menstrual blood Law in general. Comparative and uniform law. Jurisprudence K1-7720 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4219a995e8034c19bc640b503ed9d19d
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spelling	oai:doaj.org-article:4219a995e8034c19bc640b503ed9d19d2021-12-05T12:07:52ZIdentification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA10.1186/s41935-021-00256-02090-5939https://doaj.org/article/4219a995e8034c19bc640b503ed9d19d2021-12-01T00:00:00Zhttps://doi.org/10.1186/s41935-021-00256-0https://doaj.org/toc/2090-5939Abstract Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of <0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of <0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p<0.001. Conclusions Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.Riham F. HusseinSherif Mohamed El MahdyNashwa Mohammed SagedL. RashedSherien S. GhalebSpringerOpenarticleReal-time PCR multiplexNasal secretionsSalivaMenstrual bloodLaw in general. Comparative and uniform law. JurisprudenceK1-7720Medicine (General)R5-920ENEgyptian Journal of Forensic Sciences, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Real-time PCR multiplex Nasal secretions Saliva Menstrual blood Law in general. Comparative and uniform law. Jurisprudence K1-7720 Medicine (General) R5-920
spellingShingle	Real-time PCR multiplex Nasal secretions Saliva Menstrual blood Law in general. Comparative and uniform law. Jurisprudence K1-7720 Medicine (General) R5-920 Riham F. Hussein Sherif Mohamed El Mahdy Nashwa Mohammed Saged L. Rashed Sherien S. Ghaleb Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
description	Abstract Background Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7). Results The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of <0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of <0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p<0.001. Conclusions Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.
format	article
author	Riham F. Hussein Sherif Mohamed El Mahdy Nashwa Mohammed Saged L. Rashed Sherien S. Ghaleb
author_facet	Riham F. Hussein Sherif Mohamed El Mahdy Nashwa Mohammed Saged L. Rashed Sherien S. Ghaleb
author_sort	Riham F. Hussein
title	Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
title_short	Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
title_full	Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
title_fullStr	Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
title_full_unstemmed	Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
title_sort	identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mrna
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/4219a995e8034c19bc640b503ed9d19d
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Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA

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