Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides

In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for c...

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Autor principal:	Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos
Formato:	article
Lenguaje:	EN ES
Publicado:	Pontificia Universidad Javeriana 2019
Materias:	cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/421b32452207436bbfbb6d1ecd3d13a4
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spelling	oai:doaj.org-article:421b32452207436bbfbb6d1ecd3d13a42021-11-16T16:29:55ZEnvironmental and cultured cyanobacteria as sources of Aedes aegypti larvicides10.11144/Javeriana.SC24-3.eacc0122-74832027-1352https://doaj.org/article/421b32452207436bbfbb6d1ecd3d13a42019-11-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/22416https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for controlling mosquito populations and preventing epidemic outbreaks. We collected 30 benthic cyanobacterial mats in Providencia and Rosario islands (in the Colombian Caribbean) belonging to the genera Phormidium, Symploca, Oscillatoria, Lyngbya, Pseudoanabaena, Leptolyngbya, Moorea, and Dapis. Fractions of organic extracts from the most abundant environmental samples were evaluated in three bioassays, assessing (i) larvicidal activity against A. aegypti, (ii) toxicity against the brine shrimp (Artemia salina) nauplii, and (iii) acetylcholinesterase inhibition. Non-polar fractions exhibited larvicidal activity. The polar fraction from one Dapis pleuosa extract showed larvicidal activity without being toxic against A. salina nauplii. Extracts from Moorea producens exhibited the greatest toxicity against A. aegypti larvae and A. salina nauplii. From 23 cultured cyanobacterial samples, only five grew under laboratory conditions and produced enough biomass to yield organic extracts. Of these, three extracts showed strong larvicidal activity, but only the extract from Phormidium tenue showed reduced toxicity against A. salina nauplii. We detected variation among the chemical profiles and larvicidal activity of cyanobacterial consortia depending on sites and dates of collection. Our findings suggest that despite variation in chemical profiles, extracts of marine benthic cyanobacteria can be further developed as effective control agents against insect vectors, in their larval stages. The culture of marine benthic cyanobacteria needs to be further explored to provide enough biomass leading to the identification of bioactive compounds with public health applications. Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo CastellanosPontificia Universidad Javerianaarticlecyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitorsScience (General)Q1-390ENESUniversitas Scientiarum, Vol 24, Iss 3, Pp 465-496 (2019)
institution	DOAJ
collection	DOAJ
language	EN ES
topic	cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors Science (General) Q1-390
spellingShingle	cyanobacteria; aedes aegypti; artemia salina; larvicidal activity; acetylcholinesterase inhibitors Science (General) Q1-390 Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
description	In tropical countries, the control of the mosquito Aedes aegypti is a public health priority due to its role as a vector of important viral diseases. Marine cyanobacteria are recognized as abundant sources of bioactive compounds, and they constitute a potential source of insecticides useful for controlling mosquito populations and preventing epidemic outbreaks. We collected 30 benthic cyanobacterial mats in Providencia and Rosario islands (in the Colombian Caribbean) belonging to the genera Phormidium, Symploca, Oscillatoria, Lyngbya, Pseudoanabaena, Leptolyngbya, Moorea, and Dapis. Fractions of organic extracts from the most abundant environmental samples were evaluated in three bioassays, assessing (i) larvicidal activity against A. aegypti, (ii) toxicity against the brine shrimp (Artemia salina) nauplii, and (iii) acetylcholinesterase inhibition. Non-polar fractions exhibited larvicidal activity. The polar fraction from one Dapis pleuosa extract showed larvicidal activity without being toxic against A. salina nauplii. Extracts from Moorea producens exhibited the greatest toxicity against A. aegypti larvae and A. salina nauplii. From 23 cultured cyanobacterial samples, only five grew under laboratory conditions and produced enough biomass to yield organic extracts. Of these, three extracts showed strong larvicidal activity, but only the extract from Phormidium tenue showed reduced toxicity against A. salina nauplii. We detected variation among the chemical profiles and larvicidal activity of cyanobacterial consortia depending on sites and dates of collection. Our findings suggest that despite variation in chemical profiles, extracts of marine benthic cyanobacteria can be further developed as effective control agents against insect vectors, in their larval stages. The culture of marine benthic cyanobacteria needs to be further explored to provide enough biomass leading to the identification of bioactive compounds with public health applications.
format	article
author	Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos
author_facet	Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos
author_sort	Farja I. Ayala, Laura M. Becerra, Jairo Quintana, Lina M. Bayona, Freddy A Ramos, Mónica Puyana, Fredy Duque, Leonardo Castellanos
title	Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
title_short	Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
title_full	Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
title_fullStr	Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
title_full_unstemmed	Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides
title_sort	environmental and cultured cyanobacteria as sources of aedes aegypti larvicides
publisher	Pontificia Universidad Javeriana
publishDate	2019
url	https://doaj.org/article/421b32452207436bbfbb6d1ecd3d13a4
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Environmental and cultured cyanobacteria as sources of Aedes aegypti larvicides

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