Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells

Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable probl...

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Autores principales:	Danhong Han, Jingjing Xu, Han Wang, Zhenhai Wang, Nana Yang, Fan Yang, Qundong Shen, Shengyong Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	single-cell temperature measurement temperature sensors non-interventional biosensors biochip fabrication cell temperature cell metabolism Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/421bd155de6e4e5083e6d1ccd5348d4a
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spelling	oai:doaj.org-article:421bd155de6e4e5083e6d1ccd5348d4a2021-11-25T16:55:36ZNon-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells10.3390/bios111104542079-6374https://doaj.org/article/421bd155de6e4e5083e6d1ccd5348d4a2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/454https://doaj.org/toc/2079-6374Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable problems, such as, poor stability caused by their sensitivity to the chemical composition of the solution and the limitation in their measurement time due to the short fluorescence lifetime. Here, we demonstrate a stable, non-interventional, and high-precision temperature-measurement chip that can monitor the temperature fluctuations of individual cells subject to external stimuli and over a normal cell life cycle as long as several days. To improve the temperature resolution, we designed temperature sensors made of Pd–Cr thin-film thermocouples, a freestanding Si<sub>3</sub>N<sub>4</sub> platform, and a dual-temperature control system. Our experimental results confirm the feasibility of using this cellular temperature-measurement chip to detect local temperature fluctuations of individual cells that are 0.3–1.5 K higher than the ambient temperature for HeLa cells in different proliferation cycles. In the future, we plan to integrate this chip with other single-cell technologies and apply it to research related to cellular heat-stress response.Danhong HanJingjing XuHan WangZhenhai WangNana YangFan YangQundong ShenShengyong XuMDPI AGarticlesingle-cell temperature measurementtemperature sensorsnon-interventional biosensorsbiochip fabricationcell temperaturecell metabolismBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 454, p 454 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	single-cell temperature measurement temperature sensors non-interventional biosensors biochip fabrication cell temperature cell metabolism Biotechnology TP248.13-248.65
spellingShingle	single-cell temperature measurement temperature sensors non-interventional biosensors biochip fabrication cell temperature cell metabolism Biotechnology TP248.13-248.65 Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
description	Monitoring the thermal responses of individual cells to external stimuli is essential for studies of cell metabolism, organelle function, and drug screening. Fluorescent temperature probes are usually employed to measure the temperatures of individual cells; however, they have some unavoidable problems, such as, poor stability caused by their sensitivity to the chemical composition of the solution and the limitation in their measurement time due to the short fluorescence lifetime. Here, we demonstrate a stable, non-interventional, and high-precision temperature-measurement chip that can monitor the temperature fluctuations of individual cells subject to external stimuli and over a normal cell life cycle as long as several days. To improve the temperature resolution, we designed temperature sensors made of Pd–Cr thin-film thermocouples, a freestanding Si<sub>3</sub>N<sub>4</sub> platform, and a dual-temperature control system. Our experimental results confirm the feasibility of using this cellular temperature-measurement chip to detect local temperature fluctuations of individual cells that are 0.3–1.5 K higher than the ambient temperature for HeLa cells in different proliferation cycles. In the future, we plan to integrate this chip with other single-cell technologies and apply it to research related to cellular heat-stress response.
format	article
author	Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu
author_facet	Danhong Han Jingjing Xu Han Wang Zhenhai Wang Nana Yang Fan Yang Qundong Shen Shengyong Xu
author_sort	Danhong Han
title	Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
title_short	Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
title_full	Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
title_fullStr	Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
title_full_unstemmed	Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells
title_sort	non-interventional and high-precision temperature measurement biochips for long-term monitoring the temperature fluctuations of individual cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/421bd155de6e4e5083e6d1ccd5348d4a
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_version_	1718412833476050944

Non-Interventional and High-Precision Temperature Measurement Biochips for Long-Term Monitoring the Temperature Fluctuations of Individual Cells

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