Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of Ammodaucus leucotrichus. Untargeted Metabolomics Analysis by UHPLC-MS/MS

Ammodaucus leucotrichus is a spontaneous plant endemic of the North African region. An efficient selective pressurized liquid extraction (PLE) method was optimized to concentrate neuroprotective extracts from A. leucotrichus fruits. Green solvents were tested, n...

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Autores principales:	Norelhouda Abderrezag, Jose David Sánchez-Martínez, Ouahida Louaer, Abdeslam-Hassen Meniai, Jose A. Mendiola
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Ammodaucus leucotrichus</i> bioprospecting pressurized liquid extraction UHPLC-q-TOF-MS/MS neuroprotective potential anti-inflammatory activity Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/422333573f64435285b8452c24c94330
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spelling	oai:doaj.org-article:422333573f64435285b8452c24c943302021-11-25T18:28:40ZOptimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS10.3390/molecules262269511420-3049https://doaj.org/article/422333573f64435285b8452c24c943302021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6951https://doaj.org/toc/1420-3049<i>Ammodaucus leucotrichus</i> is a spontaneous plant endemic of the North African region. An efficient selective pressurized liquid extraction (PLE) method was optimized to concentrate neuroprotective extracts from <i>A. leucotrichus</i> fruits. Green solvents were tested, namely ethanol and water, within a range of temperatures between 40 to 180 °C. Total carbohydrates and total phenolics were measured in extracts, as well as in vitro antioxidant capacity (DPPH radical scavenging), anticholinesterase (AChE) and anti-inflammatory (LOX) activities. Metabolite profiling was carried out by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-q-TOF-MS/MS), identifying 94 compounds. Multivariate analysis was performed to correlate composition with bioactivity. A remarkable effect of the temperature using water was observed: the higher temperature, the higher extraction yield, the higher total phenolic content, as well as the higher total carbohydrates content. The water extract obtained at 180 °C, 10.34 MPa and 10 min showed meaningful anti-inflammatory (IC50<sub>LOX</sub> = 39.4 µg/mL) and neuroprotective activities (IC50<sub>AChE</sub> = 55.6 µg/mL). The Principal Components Analysis (PCA) and the cluster analysis correlated these activities with the presence of carbohydrates and phenolic compounds.Norelhouda AbderrezagJose David Sánchez-MartínezOuahida LouaerAbdeslam-Hassen MeniaiJose A. MendiolaMDPI AGarticle<i>Ammodaucus leucotrichus</i>bioprospectingpressurized liquid extractionUHPLC-q-TOF-MS/MSneuroprotective potentialanti-inflammatory activityOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6951, p 6951 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Ammodaucus leucotrichus</i> bioprospecting pressurized liquid extraction UHPLC-q-TOF-MS/MS neuroprotective potential anti-inflammatory activity Organic chemistry QD241-441
spellingShingle	<i>Ammodaucus leucotrichus</i> bioprospecting pressurized liquid extraction UHPLC-q-TOF-MS/MS neuroprotective potential anti-inflammatory activity Organic chemistry QD241-441 Norelhouda Abderrezag Jose David Sánchez-Martínez Ouahida Louaer Abdeslam-Hassen Meniai Jose A. Mendiola Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
description	<i>Ammodaucus leucotrichus</i> is a spontaneous plant endemic of the North African region. An efficient selective pressurized liquid extraction (PLE) method was optimized to concentrate neuroprotective extracts from <i>A. leucotrichus</i> fruits. Green solvents were tested, namely ethanol and water, within a range of temperatures between 40 to 180 °C. Total carbohydrates and total phenolics were measured in extracts, as well as in vitro antioxidant capacity (DPPH radical scavenging), anticholinesterase (AChE) and anti-inflammatory (LOX) activities. Metabolite profiling was carried out by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-q-TOF-MS/MS), identifying 94 compounds. Multivariate analysis was performed to correlate composition with bioactivity. A remarkable effect of the temperature using water was observed: the higher temperature, the higher extraction yield, the higher total phenolic content, as well as the higher total carbohydrates content. The water extract obtained at 180 °C, 10.34 MPa and 10 min showed meaningful anti-inflammatory (IC50<sub>LOX</sub> = 39.4 µg/mL) and neuroprotective activities (IC50<sub>AChE</sub> = 55.6 µg/mL). The Principal Components Analysis (PCA) and the cluster analysis correlated these activities with the presence of carbohydrates and phenolic compounds.
format	article
author	Norelhouda Abderrezag Jose David Sánchez-Martínez Ouahida Louaer Abdeslam-Hassen Meniai Jose A. Mendiola
author_facet	Norelhouda Abderrezag Jose David Sánchez-Martínez Ouahida Louaer Abdeslam-Hassen Meniai Jose A. Mendiola
author_sort	Norelhouda Abderrezag
title	Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
title_short	Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
title_full	Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
title_fullStr	Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
title_full_unstemmed	Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS
title_sort	optimization of pressurized liquid extraction and in vitro neuroprotective evaluation of <i>ammodaucus leucotrichus.</i> untargeted metabolomics analysis by uhplc-ms/ms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/422333573f64435285b8452c24c94330
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Optimization of Pressurized Liquid Extraction and In Vitro Neuroprotective Evaluation of <i>Ammodaucus leucotrichus.</i> Untargeted Metabolomics Analysis by UHPLC-MS/MS

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