Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells

Baoan Chen1,*, Yiqiong Liang1,*, Weiwei Wu1, Jian Cheng1, Guohua Xia1, Feng Gao1, Jiahua Ding1, Chong Gao1, Zeye Shao1, Guohong Li1, Wenji Chen1, Wenlin Xu2, Xinchen Sun3, Lijie Liu4, Xiaomao Li5, Xuemei Wang61Department of Hematology; 3Department of Oncology, The Affiliated Zhongda Hospital, Clinic...

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Autores principales:	Baoan Chen, Yiqiong Liang, Weiwei Wu, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/422380f9ad064731936d82e0ef28556c
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spelling	oai:doaj.org-article:422380f9ad064731936d82e0ef28556c2021-12-02T02:42:03ZSynergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells1176-91141178-2013https://doaj.org/article/422380f9ad064731936d82e0ef28556c2009-11-01T00:00:00Zhttp://www.dovepress.com/synergistic-effect-of-magnetic-nanoparticles-of-fe3o4-with-gambogic-ac-a3713https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Baoan Chen1,, Yiqiong Liang1,, Weiwei Wu1, Jian Cheng1, Guohua Xia1, Feng Gao1, Jiahua Ding1, Chong Gao1, Zeye Shao1, Guohong Li1, Wenji Chen1, Wenlin Xu2, Xinchen Sun3, Lijie Liu4, Xiaomao Li5, Xuemei Wang61Department of Hematology; 3Department of Oncology, The Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Hematology, The Affiliated People&rsquo;s Hospital, Jiangsu University, Zhenjiang, People&rsquo;s Republic of China; 4Institution of Physiology, Southeast University, Nanjing, People&rsquo;s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, Germany; 6State Key Lab of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, Peoples Republic of China; *These authors have contributed equally to this workAbstract: Gambogic acid (GA) has a significant anticancer effect on a wide variety of solid tumors. Recently, many nanoparticles have been introduced as drug-delivery systems to enhance the efficiency of anticancer drug delivery. The aim of this study was to investigate the potential benefit of combination therapy with GA and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4). The proliferation of K562 cells and their cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscope and flow cytometry, respectively. Furthermore, realtime polymerase chain reaction and Western blotting analyses were performed to examine gene transcription and protein expression, respectively. The results showed that MNPs-Fe3O4 dramatically enhanced GA-induced cytotoxicity and apoptosis in K562 cells. The typical morphological features of apoptosis treated with GA and MNPs-Fe3O4 were observed under an optical microscope and a fluorescence microscope, respectively. The transcription of caspase-3 and bax gene in the group treated with GA and MNPs-Fe3O4 was higher than that in the GA-alone group or MNPs-Fe3O4-alone group, but the transcription of bcl-2, nuclear factor-&kappa;B, and survivin degraded as did the expression of corresponding proteins in K562 cells. Our data suggests a potential clinical application of a combination of GA and MNPs-Fe3O4 in leukemia therapy.Keywords: gambogic acid, magnetic nanoparticles of Fe3O4, traditional Chinese medicine, K562 leukemia cells, apoptosis Baoan ChenYiqiong LiangWeiwei Wuet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 251-259 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Baoan Chen Yiqiong Liang Weiwei Wu et al Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
description	Baoan Chen1,, Yiqiong Liang1,, Weiwei Wu1, Jian Cheng1, Guohua Xia1, Feng Gao1, Jiahua Ding1, Chong Gao1, Zeye Shao1, Guohong Li1, Wenji Chen1, Wenlin Xu2, Xinchen Sun3, Lijie Liu4, Xiaomao Li5, Xuemei Wang61Department of Hematology; 3Department of Oncology, The Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of China; 2Department of Hematology, The Affiliated People&rsquo;s Hospital, Jiangsu University, Zhenjiang, People&rsquo;s Republic of China; 4Institution of Physiology, Southeast University, Nanjing, People&rsquo;s Republic of China; 5Department of Physics, University of Saarland, Saarbruechen, Germany; 6State Key Lab of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, Peoples Republic of China; *These authors have contributed equally to this workAbstract: Gambogic acid (GA) has a significant anticancer effect on a wide variety of solid tumors. Recently, many nanoparticles have been introduced as drug-delivery systems to enhance the efficiency of anticancer drug delivery. The aim of this study was to investigate the potential benefit of combination therapy with GA and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4). The proliferation of K562 cells and their cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscope and flow cytometry, respectively. Furthermore, realtime polymerase chain reaction and Western blotting analyses were performed to examine gene transcription and protein expression, respectively. The results showed that MNPs-Fe3O4 dramatically enhanced GA-induced cytotoxicity and apoptosis in K562 cells. The typical morphological features of apoptosis treated with GA and MNPs-Fe3O4 were observed under an optical microscope and a fluorescence microscope, respectively. The transcription of caspase-3 and bax gene in the group treated with GA and MNPs-Fe3O4 was higher than that in the GA-alone group or MNPs-Fe3O4-alone group, but the transcription of bcl-2, nuclear factor-&kappa;B, and survivin degraded as did the expression of corresponding proteins in K562 cells. Our data suggests a potential clinical application of a combination of GA and MNPs-Fe3O4 in leukemia therapy.Keywords: gambogic acid, magnetic nanoparticles of Fe3O4, traditional Chinese medicine, K562 leukemia cells, apoptosis
format	article
author	Baoan Chen Yiqiong Liang Weiwei Wu et al
author_facet	Baoan Chen Yiqiong Liang Weiwei Wu et al
author_sort	Baoan Chen
title	Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
title_short	Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
title_full	Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
title_fullStr	Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
title_full_unstemmed	Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells
title_sort	synergistic effect of magnetic nanoparticles of fe3o4 with gambogic acid on apoptosis of k562 leukemia cells
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/422380f9ad064731936d82e0ef28556c
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Synergistic effect of magnetic nanoparticles of Fe3O4 with gambogic acid on apoptosis of K562 leukemia cells

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