The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor

The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor

Fei Tong, Xiangyuan Tang, Xin Li, Wenquan Xia, Daojun Liu Department of Chemistry, Medical College, Shantou University, Shantou, People’s Republic of China Abstract: The aim of this study was to observe the therapeutic effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly...

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Autores principales: Tong F, Tang XY, Li X, Xia WQ, Liu DJ
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
insulin
PEG-b-(PELG-g-PLL)
RI/RILI
HIF-1
VEGF
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/4223a494e4e7425aa12cefb913700223
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spelling oai:doaj.org-article:4223a494e4e7425aa12cefb9137002232021-12-02T02:13:09ZThe effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor1178-2013https://doaj.org/article/4223a494e4e7425aa12cefb9137002232016-04-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-insulin-loaded-linear-polyethylene-glycol-brush-like-pol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Tong, Xiangyuan Tang, Xin Li, Wenquan Xia, Daojun Liu Department of Chemistry, Medical College, Shantou University, Shantou, People’s Republic of China Abstract: The aim of this study was to observe the therapeutic effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer poly(ethylene glycol)-b-(poly(ethylenediamine L-glutamate)-g-poly(L-lysine)) (PEG-b-(PELG-g-PLL) on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor (HIF) as compared to free insulin. Sprague Dawley rats were pretreated with 30 U/kg insulin or insulin/PEG-b-(PELG-g-PLL) complex, and then subjected to 45 minutes of ischemia and 24 hours of reperfusion. The blood and lungs were collected, the level of serum creatinine and blood urea nitrogen were measured, and the dry/wet lung ratios, the activity of superoxide dismutase and myeloperoxidase, the content of methane dicarboxylic aldehyde and tumor necrosis factor-α, and the expression of HIF-1α and vascular endothelial growth factor (VEGF) were measured in pulmonary tissues. Both insulin and insulin/PEG-b-(PELG-g-PLL) preconditioning improved the recovery of renal function, reduced pulmonary oxidative stress injury, restrained inflammatory damage, and downregulated the expression of HIF-1α and VEGF as compared to ischemia/reperfusion group, while insulin/PEG-b-(PELG-g-PLL) significantly improved this effect. Keywords: insulin, block copolymer, RI/RILI, HIF-1α, VEGFTong FTang XYLi XXia WQLiu DJDove Medical PressarticleinsulinPEG-b-(PELG-g-PLL)RI/RILIHIF-1VEGFMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1717-1730 (2016)
institution DOAJ
collection DOAJ
language EN
topic insulin
PEG-b-(PELG-g-PLL)
RI/RILI
HIF-1
VEGF
Medicine (General)
R5-920
spellingShingle insulin
PEG-b-(PELG-g-PLL)
RI/RILI
HIF-1
VEGF
Medicine (General)
R5-920
Tong F
Tang XY
Li X
Xia WQ
Liu DJ
The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
description Fei Tong, Xiangyuan Tang, Xin Li, Wenquan Xia, Daojun Liu Department of Chemistry, Medical College, Shantou University, Shantou, People’s Republic of China Abstract: The aim of this study was to observe the therapeutic effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer poly(ethylene glycol)-b-(poly(ethylenediamine L-glutamate)-g-poly(L-lysine)) (PEG-b-(PELG-g-PLL) on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor (HIF) as compared to free insulin. Sprague Dawley rats were pretreated with 30 U/kg insulin or insulin/PEG-b-(PELG-g-PLL) complex, and then subjected to 45 minutes of ischemia and 24 hours of reperfusion. The blood and lungs were collected, the level of serum creatinine and blood urea nitrogen were measured, and the dry/wet lung ratios, the activity of superoxide dismutase and myeloperoxidase, the content of methane dicarboxylic aldehyde and tumor necrosis factor-α, and the expression of HIF-1α and vascular endothelial growth factor (VEGF) were measured in pulmonary tissues. Both insulin and insulin/PEG-b-(PELG-g-PLL) preconditioning improved the recovery of renal function, reduced pulmonary oxidative stress injury, restrained inflammatory damage, and downregulated the expression of HIF-1α and VEGF as compared to ischemia/reperfusion group, while insulin/PEG-b-(PELG-g-PLL) significantly improved this effect. Keywords: insulin, block copolymer, RI/RILI, HIF-1α, VEGF
format article
author Tong F
Tang XY
Li X
Xia WQ
Liu DJ
author_facet Tong F
Tang XY
Li X
Xia WQ
Liu DJ
author_sort Tong F
title The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
title_short The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
title_full The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
title_fullStr The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
title_full_unstemmed The effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(L-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
title_sort effect of insulin-loaded linear poly(ethylene glycol)-brush-like poly(l-lysine) block copolymer on renal ischemia/reperfusion-induced lung injury through downregulating hypoxia-inducible factor
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/4223a494e4e7425aa12cefb913700223
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