Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China

Abstract There is currently a pandemic of pseudorabies virus (PRV) variant strains in China. Despite extensive research on PRV variant strains in the past two years, few studies have investigated PRV pathogenicity-related genes. To determine which gene(s) is/are linked to PRV virulence, ten putative...

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Autores principales:	Yan-Dong Tang, Ji-Ting Liu, Tong-Yun Wang, Ming-Xia Sun, Zhi-Jun Tian, Xue-Hui Cai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/422b0939b2094770be86c52bedf828fc
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spelling	oai:doaj.org-article:422b0939b2094770be86c52bedf828fc2021-12-02T11:40:44ZComparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China10.1038/s41598-017-08269-32045-2322https://doaj.org/article/422b0939b2094770be86c52bedf828fc2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08269-3https://doaj.org/toc/2045-2322Abstract There is currently a pandemic of pseudorabies virus (PRV) variant strains in China. Despite extensive research on PRV variant strains in the past two years, few studies have investigated PRV pathogenicity-related genes. To determine which gene(s) is/are linked to PRV virulence, ten putative virulence genes were knocked out using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 technology. The pathogenicity of these mutants was evaluated in a mouse model. Our results demonstrated that of the ten tested genes, the thymidine kinase (TK) and glycoprotein M (gM) knockout mutants displayed significantly reduced virulence. However, mutants of other putative virulence genes, such as glycoprotein E (gE), glycoprotein I (gI), Us2, Us9, Us3, glycoprotein G (gG), glycoprotein N (gN) and early protein 0 (EP0), did not exhibit significantly reduced virulence compared to that of the wild-type PRV. To our knowledge, this study is the first to compare virulence genes from the current pandemic PRV variant strain. This study will provide a valuable reference for scientists to design effective live attenuated vaccines in the future.Yan-Dong TangJi-Ting LiuTong-Yun WangMing-Xia SunZhi-Jun TianXue-Hui CaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan-Dong Tang Ji-Ting Liu Tong-Yun Wang Ming-Xia Sun Zhi-Jun Tian Xue-Hui Cai Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
description	Abstract There is currently a pandemic of pseudorabies virus (PRV) variant strains in China. Despite extensive research on PRV variant strains in the past two years, few studies have investigated PRV pathogenicity-related genes. To determine which gene(s) is/are linked to PRV virulence, ten putative virulence genes were knocked out using clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 technology. The pathogenicity of these mutants was evaluated in a mouse model. Our results demonstrated that of the ten tested genes, the thymidine kinase (TK) and glycoprotein M (gM) knockout mutants displayed significantly reduced virulence. However, mutants of other putative virulence genes, such as glycoprotein E (gE), glycoprotein I (gI), Us2, Us9, Us3, glycoprotein G (gG), glycoprotein N (gN) and early protein 0 (EP0), did not exhibit significantly reduced virulence compared to that of the wild-type PRV. To our knowledge, this study is the first to compare virulence genes from the current pandemic PRV variant strain. This study will provide a valuable reference for scientists to design effective live attenuated vaccines in the future.
format	article
author	Yan-Dong Tang Ji-Ting Liu Tong-Yun Wang Ming-Xia Sun Zhi-Jun Tian Xue-Hui Cai
author_facet	Yan-Dong Tang Ji-Ting Liu Tong-Yun Wang Ming-Xia Sun Zhi-Jun Tian Xue-Hui Cai
author_sort	Yan-Dong Tang
title	Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
title_short	Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
title_full	Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
title_fullStr	Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
title_full_unstemmed	Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China
title_sort	comparison of pathogenicity-related genes in the current pseudorabies virus outbreak in china
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/422b0939b2094770be86c52bedf828fc
work_keys_str_mv	AT yandongtang comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina AT jitingliu comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina AT tongyunwang comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina AT mingxiasun comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina AT zhijuntian comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina AT xuehuicai comparisonofpathogenicityrelatedgenesinthecurrentpseudorabiesvirusoutbreakinchina
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Comparison of Pathogenicity-Related Genes in the Current Pseudorabies Virus Outbreak in China

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