Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling

Objectives: Evaluation of Streptomyces isolated from Udhagamandalam, The Nilgiris, Tamilnadu, India for the biocontrol potential against the phytopathogen Ralstonia solanacearum and its metabolite profiling. Methods: Streptomyces strains were screened for plant growth promotion and antagonistic acti...

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Autores principales:	Manigundan Kaari, Jerrine Joseph, Radhakrishnan Manikkam, Ayswarya Sreenivasan, Gopikrishnan Venugopal
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Streptomyces 2,4-Di-tert-butylphenol Bioorganic fertilizer Ralstonia solanacearum Tomato Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/4230ba9d9cbd4bd79feec1c7708f2ff0
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spelling	oai:doaj.org-article:4230ba9d9cbd4bd79feec1c7708f2ff02021-11-18T04:44:35ZBiological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling1018-364710.1016/j.jksus.2021.101688https://doaj.org/article/4230ba9d9cbd4bd79feec1c7708f2ff02022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003505https://doaj.org/toc/1018-3647Objectives: Evaluation of Streptomyces isolated from Udhagamandalam, The Nilgiris, Tamilnadu, India for the biocontrol potential against the phytopathogen Ralstonia solanacearum and its metabolite profiling. Methods: Streptomyces strains were screened for plant growth promotion and antagonistic activity against R. solanacearum in in-vitro. The metabolite profiling of strain UT4A49 was studied through gas chromatography coupled with mass spectroscopy (GC–MS) analysis. Further, the identified lead compound 2,4-Di-tert-butylphenol (2,4-DTBP) was analyzed for its mechanism of action through in-silico docking study. The bioorganic fertilizer (BOF) amended potential Streptomyces strain UT4A49 was studied for biocontrol activity against R. solanacearum on tomato seedlings through pot culture experiments. Results: The Streptomyces strain UT4A49 showed the promising production of indole acetic acid (IAA) (76.0 ± 2.0 µg/ml), siderophore, ammonia, amylase, protease, cellulase as well as it solubilized the phosphate in-vitro. The strain UT4A49 showed strong in vitro antagonistic activity against R. solanacearum with the inhibition zone of 19.83 ± 0.44 mm in diameter. The isolated major compound, 2,4-Di-tert-butylphenol was showed strong inhibition against R. solanacearum. In silico docking study revealed that the 2,4-DTBP showed lower binding energy score with all targeted proteins which found better protein–ligand binding stability. In the pot experiment, biocontrol activity of BOF amended Streptomyces strain UT4A49 showed significant biocontrol efficacy was 78.5% against R. solanacearum in tomato seedlings. The strain UT4A49 was identified as Streptomyces sp. based on its morphological and molecular properties. Conclusions: The detailed literature survey revealed that this is the first report on strong bioactive properties of 2,4-DTBP against R. solanacearum. This present study proved that bioorganic fertilizer amended with Streptomyces sp. UT4A49 is a promising biocontrol agent for tomato bacterial disease control.Manigundan KaariJerrine JosephRadhakrishnan ManikkamAyswarya SreenivasanGopikrishnan VenugopalElsevierarticleStreptomyces2,4-Di-tert-butylphenolBioorganic fertilizerRalstonia solanacearumTomatoScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101688- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptomyces 2,4-Di-tert-butylphenol Bioorganic fertilizer Ralstonia solanacearum Tomato Science (General) Q1-390
spellingShingle	Streptomyces 2,4-Di-tert-butylphenol Bioorganic fertilizer Ralstonia solanacearum Tomato Science (General) Q1-390 Manigundan Kaari Jerrine Joseph Radhakrishnan Manikkam Ayswarya Sreenivasan Gopikrishnan Venugopal Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
description	Objectives: Evaluation of Streptomyces isolated from Udhagamandalam, The Nilgiris, Tamilnadu, India for the biocontrol potential against the phytopathogen Ralstonia solanacearum and its metabolite profiling. Methods: Streptomyces strains were screened for plant growth promotion and antagonistic activity against R. solanacearum in in-vitro. The metabolite profiling of strain UT4A49 was studied through gas chromatography coupled with mass spectroscopy (GC–MS) analysis. Further, the identified lead compound 2,4-Di-tert-butylphenol (2,4-DTBP) was analyzed for its mechanism of action through in-silico docking study. The bioorganic fertilizer (BOF) amended potential Streptomyces strain UT4A49 was studied for biocontrol activity against R. solanacearum on tomato seedlings through pot culture experiments. Results: The Streptomyces strain UT4A49 showed the promising production of indole acetic acid (IAA) (76.0 ± 2.0 µg/ml), siderophore, ammonia, amylase, protease, cellulase as well as it solubilized the phosphate in-vitro. The strain UT4A49 showed strong in vitro antagonistic activity against R. solanacearum with the inhibition zone of 19.83 ± 0.44 mm in diameter. The isolated major compound, 2,4-Di-tert-butylphenol was showed strong inhibition against R. solanacearum. In silico docking study revealed that the 2,4-DTBP showed lower binding energy score with all targeted proteins which found better protein–ligand binding stability. In the pot experiment, biocontrol activity of BOF amended Streptomyces strain UT4A49 showed significant biocontrol efficacy was 78.5% against R. solanacearum in tomato seedlings. The strain UT4A49 was identified as Streptomyces sp. based on its morphological and molecular properties. Conclusions: The detailed literature survey revealed that this is the first report on strong bioactive properties of 2,4-DTBP against R. solanacearum. This present study proved that bioorganic fertilizer amended with Streptomyces sp. UT4A49 is a promising biocontrol agent for tomato bacterial disease control.
format	article
author	Manigundan Kaari Jerrine Joseph Radhakrishnan Manikkam Ayswarya Sreenivasan Gopikrishnan Venugopal
author_facet	Manigundan Kaari Jerrine Joseph Radhakrishnan Manikkam Ayswarya Sreenivasan Gopikrishnan Venugopal
author_sort	Manigundan Kaari
title	Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
title_short	Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
title_full	Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
title_fullStr	Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
title_full_unstemmed	Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling
title_sort	biological control of streptomyces sp. ut4a49 to suppress tomato bacterial wilt disease and its metabolite profiling
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/4230ba9d9cbd4bd79feec1c7708f2ff0
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Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling

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