Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.

The establishment and succession of bacterial communities in infants may have a profound impact in their health, but information about the composition of meconium microbiota and its evolution in hospitalized preterm infants is scarce. In this context, the objective of this work was to characterize t...

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Autores principales:	Laura Moles, Marta Gómez, Hans Heilig, Gerardo Bustos, Susana Fuentes, Willem de Vos, Leónides Fernández, Juan M Rodríguez, Esther Jiménez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/423d4f169a77422cb260bccabcfd9aab
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spelling	oai:doaj.org-article:423d4f169a77422cb260bccabcfd9aab2021-11-18T07:39:33ZBacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.1932-620310.1371/journal.pone.0066986https://doaj.org/article/423d4f169a77422cb260bccabcfd9aab2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840569/?tool=EBIhttps://doaj.org/toc/1932-6203The establishment and succession of bacterial communities in infants may have a profound impact in their health, but information about the composition of meconium microbiota and its evolution in hospitalized preterm infants is scarce. In this context, the objective of this work was to characterize the microbiota of meconium and fecal samples obtained during the first 3 weeks of life from 14 donors using culture and molecular techniques, including DGGE and the Human Intestinal Tract Chip (HITChip) analysis of 16S rRNA amplicons. Culture techniques offer a quantification of cultivable bacteria and allow further study of the isolate, while molecular techniques provide deeper information on bacterial diversity. Culture and HITChip results were very similar but the former showed lower sensitivity. Inter-individual differences were detected in the microbiota profiles although the meconium microbiota was peculiar and distinct from that of fecal samples. Bacilli and other Firmicutes were the main bacteria groups detected in meconium while Proteobacteria dominated in the fecal samples. Culture technique showed that Staphylococcus predominated in meconium and that Enterococcus, together with Gram-negative bacteria such as Escherichia coli, Escherichia fergusonii, Klebsiella pneumoniae and Serratia marcescens, was more abundant in fecal samples. In addition, HITChip results showed the prevalence of bacteria related to Lactobacillus plantarum and Streptococcus mitis in meconium samples whereas those related to Enterococcus, Escherichia coli, Klebsiella pneumoniae and Yersinia predominated in the 3(rd) week feces. This study highlights that spontaneously-released meconium of preterm neonates contains a specific microbiota that differs from that of feces obtained after the first week of life. Our findings indicate that the presence of Serratia was strongly associated with a higher degree of immaturity and other hospital-related parameters, including antibiotherapy and mechanical ventilation.Laura MolesMarta GómezHans HeiligGerardo BustosSusana FuentesWillem de VosLeónides FernándezJuan M RodríguezEsther JiménezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66986 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Moles Marta Gómez Hans Heilig Gerardo Bustos Susana Fuentes Willem de Vos Leónides Fernández Juan M Rodríguez Esther Jiménez Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
description	The establishment and succession of bacterial communities in infants may have a profound impact in their health, but information about the composition of meconium microbiota and its evolution in hospitalized preterm infants is scarce. In this context, the objective of this work was to characterize the microbiota of meconium and fecal samples obtained during the first 3 weeks of life from 14 donors using culture and molecular techniques, including DGGE and the Human Intestinal Tract Chip (HITChip) analysis of 16S rRNA amplicons. Culture techniques offer a quantification of cultivable bacteria and allow further study of the isolate, while molecular techniques provide deeper information on bacterial diversity. Culture and HITChip results were very similar but the former showed lower sensitivity. Inter-individual differences were detected in the microbiota profiles although the meconium microbiota was peculiar and distinct from that of fecal samples. Bacilli and other Firmicutes were the main bacteria groups detected in meconium while Proteobacteria dominated in the fecal samples. Culture technique showed that Staphylococcus predominated in meconium and that Enterococcus, together with Gram-negative bacteria such as Escherichia coli, Escherichia fergusonii, Klebsiella pneumoniae and Serratia marcescens, was more abundant in fecal samples. In addition, HITChip results showed the prevalence of bacteria related to Lactobacillus plantarum and Streptococcus mitis in meconium samples whereas those related to Enterococcus, Escherichia coli, Klebsiella pneumoniae and Yersinia predominated in the 3(rd) week feces. This study highlights that spontaneously-released meconium of preterm neonates contains a specific microbiota that differs from that of feces obtained after the first week of life. Our findings indicate that the presence of Serratia was strongly associated with a higher degree of immaturity and other hospital-related parameters, including antibiotherapy and mechanical ventilation.
format	article
author	Laura Moles Marta Gómez Hans Heilig Gerardo Bustos Susana Fuentes Willem de Vos Leónides Fernández Juan M Rodríguez Esther Jiménez
author_facet	Laura Moles Marta Gómez Hans Heilig Gerardo Bustos Susana Fuentes Willem de Vos Leónides Fernández Juan M Rodríguez Esther Jiménez
author_sort	Laura Moles
title	Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
title_short	Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
title_full	Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
title_fullStr	Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
title_full_unstemmed	Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
title_sort	bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/423d4f169a77422cb260bccabcfd9aab
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Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life.

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