WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

Abstract The vascular wall within adipose tissue is a source of mesenchymal progenitors, referred to as perivascular stem/stromal cells (PSC). PSC are isolated via fluorescence activated cell sorting (FACS), and defined as a bipartite population of pericytes and adventitial progenitor cells (APCs)....

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Autores principales:	Carolyn A. Meyers, Jiajia Xu, Greg Asatrian, Catherine Ding, Jia Shen, Kristen Broderick, Kang Ting, Chia Soo, Bruno Peault, Aaron W. James
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Perivascular Niche FACS Purification Segmental Spinal Fusion Adipogenic Differentiation Conditions Osteogenic Differentiation Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42416fecc8304721b35bf1a2a3b626bd
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spelling	oai:doaj.org-article:42416fecc8304721b35bf1a2a3b626bd2021-12-02T15:08:18ZWISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells10.1038/s41598-018-34143-x2045-2322https://doaj.org/article/42416fecc8304721b35bf1a2a3b626bd2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34143-xhttps://doaj.org/toc/2045-2322Abstract The vascular wall within adipose tissue is a source of mesenchymal progenitors, referred to as perivascular stem/stromal cells (PSC). PSC are isolated via fluorescence activated cell sorting (FACS), and defined as a bipartite population of pericytes and adventitial progenitor cells (APCs). Those factors that promote the differentiation of PSC into bone or fat cell types are not well understood. Here, we observed high expression of WISP-1 among human PSC in vivo, after purification, and upon transplantation in a bone defect. Next, modulation of WISP-1 expression was performed, using WISP-1 overexpression, WISP-1 protein, or WISP-1 siRNA. Results demonstrated that WISP-1 is expressed in the perivascular niche, and high expression is maintained after purification of PSC, and upon transplantation in a bone microenvironment. In vitro studies demonstrate that WISP-1 has pro-osteogenic/anti-adipocytic effects in human PSC, and that regulation of BMP signaling activity may underlie these effects. In summary, our results demonstrate the importance of the matricellular protein WISP-1 in regulation of the differentiation of human stem cell types within the perivascular niche. WISP-1 signaling upregulation may be of future benefit in cell therapy mediated bone tissue engineering, for the healing of bone defects or other orthopaedic applications.Carolyn A. MeyersJiajia XuGreg AsatrianCatherine DingJia ShenKristen BroderickKang TingChia SooBruno PeaultAaron W. JamesNature PortfolioarticlePerivascular NicheFACS PurificationSegmental Spinal FusionAdipogenic Differentiation ConditionsOsteogenic DifferentiationMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Perivascular Niche FACS Purification Segmental Spinal Fusion Adipogenic Differentiation Conditions Osteogenic Differentiation Medicine R Science Q
spellingShingle	Perivascular Niche FACS Purification Segmental Spinal Fusion Adipogenic Differentiation Conditions Osteogenic Differentiation Medicine R Science Q Carolyn A. Meyers Jiajia Xu Greg Asatrian Catherine Ding Jia Shen Kristen Broderick Kang Ting Chia Soo Bruno Peault Aaron W. James WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
description	Abstract The vascular wall within adipose tissue is a source of mesenchymal progenitors, referred to as perivascular stem/stromal cells (PSC). PSC are isolated via fluorescence activated cell sorting (FACS), and defined as a bipartite population of pericytes and adventitial progenitor cells (APCs). Those factors that promote the differentiation of PSC into bone or fat cell types are not well understood. Here, we observed high expression of WISP-1 among human PSC in vivo, after purification, and upon transplantation in a bone defect. Next, modulation of WISP-1 expression was performed, using WISP-1 overexpression, WISP-1 protein, or WISP-1 siRNA. Results demonstrated that WISP-1 is expressed in the perivascular niche, and high expression is maintained after purification of PSC, and upon transplantation in a bone microenvironment. In vitro studies demonstrate that WISP-1 has pro-osteogenic/anti-adipocytic effects in human PSC, and that regulation of BMP signaling activity may underlie these effects. In summary, our results demonstrate the importance of the matricellular protein WISP-1 in regulation of the differentiation of human stem cell types within the perivascular niche. WISP-1 signaling upregulation may be of future benefit in cell therapy mediated bone tissue engineering, for the healing of bone defects or other orthopaedic applications.
format	article
author	Carolyn A. Meyers Jiajia Xu Greg Asatrian Catherine Ding Jia Shen Kristen Broderick Kang Ting Chia Soo Bruno Peault Aaron W. James
author_facet	Carolyn A. Meyers Jiajia Xu Greg Asatrian Catherine Ding Jia Shen Kristen Broderick Kang Ting Chia Soo Bruno Peault Aaron W. James
author_sort	Carolyn A. Meyers
title	WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
title_short	WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
title_full	WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
title_fullStr	WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
title_full_unstemmed	WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells
title_sort	wisp-1 drives bone formation at the expense of fat formation in human perivascular stem cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/42416fecc8304721b35bf1a2a3b626bd
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WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells

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