Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae

Abstract The aim of this study was to explore the possible antibacterial components of Salvia miltiorrhizae on Pseudomonas aeruginosa using a combination of chemical fingerprint and bioactivity evaluation. The chemical fingerprints of 32 batches of S. miltiorrhizae samples from different sources wer...

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Autores principales:	Wei-Jun Kong, Shan-Shan Zhang, Yan-Ling Zhao, Ming-Quan Wu, Ping Chen, Xiao-Ru Wu, Xin-Ping Ma, Wei-Ying Guo, Mei-Hua Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42425ceddcd4452f82066a915e7d8006
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spelling	oai:doaj.org-article:42425ceddcd4452f82066a915e7d80062021-12-02T11:40:12ZCombination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae10.1038/s41598-017-08377-02045-2322https://doaj.org/article/42425ceddcd4452f82066a915e7d80062017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08377-0https://doaj.org/toc/2045-2322Abstract The aim of this study was to explore the possible antibacterial components of Salvia miltiorrhizae on Pseudomonas aeruginosa using a combination of chemical fingerprint and bioactivity evaluation. The chemical fingerprints of 32 batches of S. miltiorrhizae samples from different sources were developed using high-performance liquid chromatography with diode array detection, and then were evaluated by similarity analysis and hierarchical clustering analysis. Anti-P. aeruginosa activity was determined by microcalorimetry. Some crucial thermokinetic parameters obtained from the heat-flow power-time curves of P. aeruginosa growth in the absence or presence of these S. miltiorrhizae samples were evaluated using principal component analysis. Thereafter, multiple linear regression analysis was used to analyze the fingerprint-activity relationship between the chemical fingerprints and anti-P. aeruginosa activity. This established the related equation between the inhibition ratio (I, %) of S. miltiorrhizae samples on P. aeruginosa and the peak areas of the common peaks. The results showed that the 32S. miltiorrhizae samples could be grouped into three clusters according to their chemical fingerprints and anti-P. aeruginosa activities. Protocatechualdehyde, salvianolic acid B, together with three unidentified compounds might be the major components that contributed largely to the antibacterial properties of S. miltiorrhizae and should be the focus of S. miltiorrhizae quality control. Thus, this study provided a preferred way for exploring the bioactive components of medicinal plants.Wei-Jun KongShan-Shan ZhangYan-Ling ZhaoMing-Quan WuPing ChenXiao-Ru WuXin-Ping MaWei-Ying GuoMei-Hua YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Jun Kong Shan-Shan Zhang Yan-Ling Zhao Ming-Quan Wu Ping Chen Xiao-Ru Wu Xin-Ping Ma Wei-Ying Guo Mei-Hua Yang Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
description	Abstract The aim of this study was to explore the possible antibacterial components of Salvia miltiorrhizae on Pseudomonas aeruginosa using a combination of chemical fingerprint and bioactivity evaluation. The chemical fingerprints of 32 batches of S. miltiorrhizae samples from different sources were developed using high-performance liquid chromatography with diode array detection, and then were evaluated by similarity analysis and hierarchical clustering analysis. Anti-P. aeruginosa activity was determined by microcalorimetry. Some crucial thermokinetic parameters obtained from the heat-flow power-time curves of P. aeruginosa growth in the absence or presence of these S. miltiorrhizae samples were evaluated using principal component analysis. Thereafter, multiple linear regression analysis was used to analyze the fingerprint-activity relationship between the chemical fingerprints and anti-P. aeruginosa activity. This established the related equation between the inhibition ratio (I, %) of S. miltiorrhizae samples on P. aeruginosa and the peak areas of the common peaks. The results showed that the 32S. miltiorrhizae samples could be grouped into three clusters according to their chemical fingerprints and anti-P. aeruginosa activities. Protocatechualdehyde, salvianolic acid B, together with three unidentified compounds might be the major components that contributed largely to the antibacterial properties of S. miltiorrhizae and should be the focus of S. miltiorrhizae quality control. Thus, this study provided a preferred way for exploring the bioactive components of medicinal plants.
format	article
author	Wei-Jun Kong Shan-Shan Zhang Yan-Ling Zhao Ming-Quan Wu Ping Chen Xiao-Ru Wu Xin-Ping Ma Wei-Ying Guo Mei-Hua Yang
author_facet	Wei-Jun Kong Shan-Shan Zhang Yan-Ling Zhao Ming-Quan Wu Ping Chen Xiao-Ru Wu Xin-Ping Ma Wei-Ying Guo Mei-Hua Yang
author_sort	Wei-Jun Kong
title	Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
title_short	Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
title_full	Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
title_fullStr	Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
title_full_unstemmed	Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae
title_sort	combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of salvia miltiorrhizae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/42425ceddcd4452f82066a915e7d8006
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Combination of chemical fingerprint and bioactivity evaluation to explore the antibacterial components of Salvia miltiorrhizae

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