Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, r...

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Autores principales:	Jianyi Wang, Qi Wang, Yongjun Zheng, Tao Peng, Kai Yao, Sanlei Xie, Xiya Zhang, Xi Xia, Jiancheng Li, Haiyang Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	lateral flow immunoassay quantitative detection fluorescent microsphere chloramphenicol thiamphenicol florfenicol milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4249f6507c67419b83ab3962f8ae4e4f
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spelling	oai:doaj.org-article:4249f6507c67419b83ab3962f8ae4e4f2021-11-26T11:19:46ZDevelopment of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk0954-01051465-344310.1080/09540105.2017.1359498https://doaj.org/article/4249f6507c67419b83ab3962f8ae4e4f2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1359498https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis.Jianyi WangQi WangYongjun ZhengTao PengKai YaoSanlei XieXiya ZhangXi XiaJiancheng LiHaiyang JiangTaylor & Francis Grouparticlelateral flow immunoassayquantitative detectionfluorescent microspherechloramphenicolthiamphenicolflorfenicolmilkAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 56-66 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	lateral flow immunoassay quantitative detection fluorescent microsphere chloramphenicol thiamphenicol florfenicol milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	lateral flow immunoassay quantitative detection fluorescent microsphere chloramphenicol thiamphenicol florfenicol milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
description	A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis.
format	article
author	Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang
author_facet	Jianyi Wang Qi Wang Yongjun Zheng Tao Peng Kai Yao Sanlei Xie Xiya Zhang Xi Xia Jiancheng Li Haiyang Jiang
author_sort	Jianyi Wang
title	Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
title_short	Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
title_full	Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
title_fullStr	Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
title_full_unstemmed	Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
title_sort	development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/4249f6507c67419b83ab3962f8ae4e4f
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Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

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