Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.

In the mammalian central nervous system (CNS) an important contingent of dopaminergic neurons are localized in the substantia nigra and in the ventral tegmental area of the ventral midbrain. They constitute an anatomically and functionally heterogeneous group of cells involved in a variety of regula...

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Autores principales:	Dario Greco, Floriana Volpicelli, Antonio Di Lieto, Damiana Leo, Carla Perrone-Capano, Petri Auvinen, Umberto di Porzio
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42563001c7a2464ea65be53120afc5f7
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spelling	oai:doaj.org-article:42563001c7a2464ea65be53120afc5f72021-11-25T06:16:33ZComparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.1932-620310.1371/journal.pone.0004977https://doaj.org/article/42563001c7a2464ea65be53120afc5f72009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19305503/?tool=EBIhttps://doaj.org/toc/1932-6203In the mammalian central nervous system (CNS) an important contingent of dopaminergic neurons are localized in the substantia nigra and in the ventral tegmental area of the ventral midbrain. They constitute an anatomically and functionally heterogeneous group of cells involved in a variety of regulatory mechanisms, from locomotion to emotional/motivational behavior. Midbrain dopaminergic neuron (mDA) primary cultures represent a useful tool to study molecular mechanisms involved in their development and maintenance. Considerable information has been gathered on the mDA neurons development and maturation in vivo, as well as on the molecular features of mDA primary cultures. Here we investigated in detail the gene expression differences between the tissue of origin and ventral midbrain primary cultures enriched in mDA neurons, using microarray technique. We integrated the results based on different re-annotations of the microarray probes. By using knowledge-based gene network techniques and promoter sequence analysis, we also uncovered mechanisms that might regulate the expression of CNS genes involved in the definition of the identity of specific cell types in the ventral midbrain. We integrate bioinformatics and functional genomics, together with developmental neurobiology. Moreover, we propose guidelines for the computational analysis of microarray gene expression data. Our findings help to clarify some molecular aspects of the development and differentiation of DA neurons within the midbrain.Dario GrecoFloriana VolpicelliAntonio Di LietoDamiana LeoCarla Perrone-CapanoPetri AuvinenUmberto di PorzioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4977 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dario Greco Floriana Volpicelli Antonio Di Lieto Damiana Leo Carla Perrone-Capano Petri Auvinen Umberto di Porzio Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
description	In the mammalian central nervous system (CNS) an important contingent of dopaminergic neurons are localized in the substantia nigra and in the ventral tegmental area of the ventral midbrain. They constitute an anatomically and functionally heterogeneous group of cells involved in a variety of regulatory mechanisms, from locomotion to emotional/motivational behavior. Midbrain dopaminergic neuron (mDA) primary cultures represent a useful tool to study molecular mechanisms involved in their development and maintenance. Considerable information has been gathered on the mDA neurons development and maturation in vivo, as well as on the molecular features of mDA primary cultures. Here we investigated in detail the gene expression differences between the tissue of origin and ventral midbrain primary cultures enriched in mDA neurons, using microarray technique. We integrated the results based on different re-annotations of the microarray probes. By using knowledge-based gene network techniques and promoter sequence analysis, we also uncovered mechanisms that might regulate the expression of CNS genes involved in the definition of the identity of specific cell types in the ventral midbrain. We integrate bioinformatics and functional genomics, together with developmental neurobiology. Moreover, we propose guidelines for the computational analysis of microarray gene expression data. Our findings help to clarify some molecular aspects of the development and differentiation of DA neurons within the midbrain.
format	article
author	Dario Greco Floriana Volpicelli Antonio Di Lieto Damiana Leo Carla Perrone-Capano Petri Auvinen Umberto di Porzio
author_facet	Dario Greco Floriana Volpicelli Antonio Di Lieto Damiana Leo Carla Perrone-Capano Petri Auvinen Umberto di Porzio
author_sort	Dario Greco
title	Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
title_short	Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
title_full	Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
title_fullStr	Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
title_full_unstemmed	Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
title_sort	comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/42563001c7a2464ea65be53120afc5f7
work_keys_str_mv	AT dariogreco comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT florianavolpicelli comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT antoniodilieto comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT damianaleo comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT carlaperronecapano comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT petriauvinen comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures AT umbertodiporzio comparisonofgeneexpressionprofileinembryonicmesencephalonandneuronalprimarycultures
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Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.

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