Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections

Abstract Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-v...

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Autores principales:	Erin S. Gloag, Daniel J. Wozniak, Paul Stoodley, Luanne Hall-Stoodley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4262d7b60c064cb3a747aac8e063298b
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spelling	oai:doaj.org-article:4262d7b60c064cb3a747aac8e063298b2021-12-02T13:19:23ZMycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections10.1038/s41598-021-84525-x2045-2322https://doaj.org/article/4262d7b60c064cb3a747aac8e063298b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84525-xhttps://doaj.org/toc/2045-2322Abstract Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-variants of M. abscessus are routinely isolated from CF samples, smooth (Ma Sm) and rough (Ma Rg). These two variants display distinct colony morphologies due to the presence (Ma Sm) or absence (Ma Rg) of cell wall glycopeptidolipids (GPLs). We hypothesized that Ma Sm and Ma Rg variant biofilms might have different mechanical properties. To test this hypothesis, we performed uniaxial mechanical indentation, and shear rheometry on Ma Sm and Ma Rg colony-biofilms. We identified that Ma Rg biofilms were significantly stiffer than Ma Sm under a normal force, while Ma Sm biofilms were more pliant compared to Ma Rg, under both normal and shear forces. Furthermore, using theoretical indices of mucociliary and cough clearance, we identified that M. abscessus biofilms may be more resistant to mechanical forms of clearance from the lung, compared to another common pulmonary pathogen, Pseudomonas aeruginosa. Thus, the mechanical properties of M. abscessus biofilms may contribute to the persistent nature of pulmonary infections caused by this organism.Erin S. GloagDaniel J. WozniakPaul StoodleyLuanne Hall-StoodleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erin S. Gloag Daniel J. Wozniak Paul Stoodley Luanne Hall-Stoodley Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
description	Abstract Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-variants of M. abscessus are routinely isolated from CF samples, smooth (Ma Sm) and rough (Ma Rg). These two variants display distinct colony morphologies due to the presence (Ma Sm) or absence (Ma Rg) of cell wall glycopeptidolipids (GPLs). We hypothesized that Ma Sm and Ma Rg variant biofilms might have different mechanical properties. To test this hypothesis, we performed uniaxial mechanical indentation, and shear rheometry on Ma Sm and Ma Rg colony-biofilms. We identified that Ma Rg biofilms were significantly stiffer than Ma Sm under a normal force, while Ma Sm biofilms were more pliant compared to Ma Rg, under both normal and shear forces. Furthermore, using theoretical indices of mucociliary and cough clearance, we identified that M. abscessus biofilms may be more resistant to mechanical forms of clearance from the lung, compared to another common pulmonary pathogen, Pseudomonas aeruginosa. Thus, the mechanical properties of M. abscessus biofilms may contribute to the persistent nature of pulmonary infections caused by this organism.
format	article
author	Erin S. Gloag Daniel J. Wozniak Paul Stoodley Luanne Hall-Stoodley
author_facet	Erin S. Gloag Daniel J. Wozniak Paul Stoodley Luanne Hall-Stoodley
author_sort	Erin S. Gloag
title	Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
title_short	Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
title_full	Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
title_fullStr	Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
title_full_unstemmed	Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
title_sort	mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4262d7b60c064cb3a747aac8e063298b
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Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections

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