An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin

An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin

Emilia Galdiero,1 Antonietta Siciliano,1 Valeria Maselli,1 Renato Gesuele,1 Marco Guida,1 Domenico Fulgione,1 Stefania Galdiero,2 Lucia Lombardi,3 Annarita Falanga2 1Department of Biology, University of Naples “Federico II”, Naples, Italy; 2Department of Pharmacy and Cirpeb, Uni...

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Autores principales: Galdiero E, Siciliano A, Maselli V, Gesuele R, Guida M, Fulgione D, Galdiero S, Lombardi L, Falanga A
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
peptide
quantum dots
ecotoxicity
antimicrobial activity
oxidative stress
genotoxicity
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/42636349f22d43119690717245224f7a
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spelling oai:doaj.org-article:42636349f22d43119690717245224f7a2021-12-02T03:05:14ZAn integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin1178-2013https://doaj.org/article/42636349f22d43119690717245224f7a2016-08-01T00:00:00Zhttps://www.dovepress.com/an-integrated-study-on-antimicrobial-activity-and-ecotoxicity-of-qds-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Emilia Galdiero,1 Antonietta Siciliano,1 Valeria Maselli,1 Renato Gesuele,1 Marco Guida,1 Domenico Fulgione,1 Stefania Galdiero,2 Lucia Lombardi,3 Annarita Falanga2 1Department of Biology, University of Naples “Federico II”, Naples, Italy; 2Department of Pharmacy and Cirpeb, University of Naples “Federico II”, Naples, Italy; 3Department of Experimental Medicine, Second University of Naples, Naples, Italy Abstract: This study attempts to evaluate the antimicrobial activity and the ecotoxicity of quantum dots (QDs) alone and coated with indolicidin. To meet this objective, we tested the level of antimicrobial activity on Gram-positive and Gram-negative bacteria, and we designed an ecotoxicological battery of test systems and indicators able to detect different effects using a variety of end points. The antibacterial activity was analyzed against Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 1025), Escherichia coli (ATCC 11229), and Klebsiella pneumoniae (ATCC 10031), and the results showed an improved germicidal action of QDs-Ind. Toxicity studies on Daphnia magna indicated a decrease in toxicity for QDs-Ind compared to QDs alone, lack of bioluminescence inhibition on Vibrio fisheri, and no mutations in Salmonella typhimurium TA 100. The comet assay and oxidative stress experiments performed on D. magna showed a genotoxic and an oxidative damage with a dose–response trend. Indolicidin retained its activity when bound to QDs. We observed an enhanced activity for QDs-Ind. The presence of indolicidin on the surface of QDs was able to decrease its QDs toxicity. Keywords: peptide, quantum dots, ecotoxicity, antimicrobial activity, oxidative stress, genotoxicityGaldiero ESiciliano AMaselli VGesuele RGuida MFulgione DGaldiero SLombardi LFalanga ADove Medical Pressarticlepeptidequantum dotsecotoxicityantimicrobial activityoxidative stressgenotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4199-4211 (2016)
institution DOAJ
collection DOAJ
language EN
topic peptide
quantum dots
ecotoxicity
antimicrobial activity
oxidative stress
genotoxicity
Medicine (General)
R5-920
spellingShingle peptide
quantum dots
ecotoxicity
antimicrobial activity
oxidative stress
genotoxicity
Medicine (General)
R5-920
Galdiero E
Siciliano A
Maselli V
Gesuele R
Guida M
Fulgione D
Galdiero S
Lombardi L
Falanga A
An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
description Emilia Galdiero,1 Antonietta Siciliano,1 Valeria Maselli,1 Renato Gesuele,1 Marco Guida,1 Domenico Fulgione,1 Stefania Galdiero,2 Lucia Lombardi,3 Annarita Falanga2 1Department of Biology, University of Naples “Federico II”, Naples, Italy; 2Department of Pharmacy and Cirpeb, University of Naples “Federico II”, Naples, Italy; 3Department of Experimental Medicine, Second University of Naples, Naples, Italy Abstract: This study attempts to evaluate the antimicrobial activity and the ecotoxicity of quantum dots (QDs) alone and coated with indolicidin. To meet this objective, we tested the level of antimicrobial activity on Gram-positive and Gram-negative bacteria, and we designed an ecotoxicological battery of test systems and indicators able to detect different effects using a variety of end points. The antibacterial activity was analyzed against Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 1025), Escherichia coli (ATCC 11229), and Klebsiella pneumoniae (ATCC 10031), and the results showed an improved germicidal action of QDs-Ind. Toxicity studies on Daphnia magna indicated a decrease in toxicity for QDs-Ind compared to QDs alone, lack of bioluminescence inhibition on Vibrio fisheri, and no mutations in Salmonella typhimurium TA 100. The comet assay and oxidative stress experiments performed on D. magna showed a genotoxic and an oxidative damage with a dose–response trend. Indolicidin retained its activity when bound to QDs. We observed an enhanced activity for QDs-Ind. The presence of indolicidin on the surface of QDs was able to decrease its QDs toxicity. Keywords: peptide, quantum dots, ecotoxicity, antimicrobial activity, oxidative stress, genotoxicity
format article
author Galdiero E
Siciliano A
Maselli V
Gesuele R
Guida M
Fulgione D
Galdiero S
Lombardi L
Falanga A
author_facet Galdiero E
Siciliano A
Maselli V
Gesuele R
Guida M
Fulgione D
Galdiero S
Lombardi L
Falanga A
author_sort Galdiero E
title An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
title_short An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
title_full An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
title_fullStr An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
title_full_unstemmed An integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
title_sort integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/42636349f22d43119690717245224f7a
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