Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.

During the summer of 2012, a major Legionella pneumophila serogroup 1 outbreak occurred in Quebec City, Canada, which caused 182 declared cases of Legionnaire's disease and included 13 fatalities. Legionella pneumophila serogroup 1 isolates from 23 patients as well as from 32 cooling towers loc...

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Autores principales:	Simon Lévesque, Pier-Luc Plante, Nilmini Mendis, Philippe Cantin, Geneviève Marchand, Hugues Charest, Frédéric Raymond, Caroline Huot, Isabelle Goupil-Sormany, François Desbiens, Sébastien P Faucher, Jacques Corbeil, Cécile Tremblay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/426416e5e6024f829f201b02ae19ba92
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spelling	oai:doaj.org-article:426416e5e6024f829f201b02ae19ba922021-11-25T06:05:34ZGenomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.1932-620310.1371/journal.pone.0103852https://doaj.org/article/426416e5e6024f829f201b02ae19ba922014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25105285/?tool=EBIhttps://doaj.org/toc/1932-6203During the summer of 2012, a major Legionella pneumophila serogroup 1 outbreak occurred in Quebec City, Canada, which caused 182 declared cases of Legionnaire's disease and included 13 fatalities. Legionella pneumophila serogroup 1 isolates from 23 patients as well as from 32 cooling towers located in the vicinity of the outbreak were recovered for analysis. In addition, 6 isolates from the 1996 Quebec City outbreak and 4 isolates from patients unrelated to both outbreaks were added to allow comparison. We characterized the isolates using pulsed-field gel electrophoresis, sequence-based typing, and whole genome sequencing. The comparison of patients-isolated strains to cooling tower isolates allowed the identification of the tower that was the source of the outbreak. Legionella pneumophila strain Quebec 2012 was identified as a ST-62 by sequence-based typing methodology. Two new Legionellaceae plasmids were found only in the epidemic strain. The LVH type IV secretion system was found in the 2012 outbreak isolates but not in the ones from the 1996 outbreak and only in half of the contemporary human isolates. The epidemic strains replicated more efficiently and were more cytotoxic to human macrophages than the environmental strains tested. At least four Icm/Dot effectors in the epidemic strains were absent in the environmental strains suggesting that some effectors could impact the intracellular replication in human macrophages. Sequence-based typing and pulsed-field gel electrophoresis combined with whole genome sequencing allowed the identification and the analysis of the causative strain including its likely environmental source.Simon LévesquePier-Luc PlanteNilmini MendisPhilippe CantinGeneviève MarchandHugues CharestFrédéric RaymondCaroline HuotIsabelle Goupil-SormanyFrançois DesbiensSébastien P FaucherJacques CorbeilCécile TremblayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103852 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon Lévesque Pier-Luc Plante Nilmini Mendis Philippe Cantin Geneviève Marchand Hugues Charest Frédéric Raymond Caroline Huot Isabelle Goupil-Sormany François Desbiens Sébastien P Faucher Jacques Corbeil Cécile Tremblay Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
description	During the summer of 2012, a major Legionella pneumophila serogroup 1 outbreak occurred in Quebec City, Canada, which caused 182 declared cases of Legionnaire's disease and included 13 fatalities. Legionella pneumophila serogroup 1 isolates from 23 patients as well as from 32 cooling towers located in the vicinity of the outbreak were recovered for analysis. In addition, 6 isolates from the 1996 Quebec City outbreak and 4 isolates from patients unrelated to both outbreaks were added to allow comparison. We characterized the isolates using pulsed-field gel electrophoresis, sequence-based typing, and whole genome sequencing. The comparison of patients-isolated strains to cooling tower isolates allowed the identification of the tower that was the source of the outbreak. Legionella pneumophila strain Quebec 2012 was identified as a ST-62 by sequence-based typing methodology. Two new Legionellaceae plasmids were found only in the epidemic strain. The LVH type IV secretion system was found in the 2012 outbreak isolates but not in the ones from the 1996 outbreak and only in half of the contemporary human isolates. The epidemic strains replicated more efficiently and were more cytotoxic to human macrophages than the environmental strains tested. At least four Icm/Dot effectors in the epidemic strains were absent in the environmental strains suggesting that some effectors could impact the intracellular replication in human macrophages. Sequence-based typing and pulsed-field gel electrophoresis combined with whole genome sequencing allowed the identification and the analysis of the causative strain including its likely environmental source.
format	article
author	Simon Lévesque Pier-Luc Plante Nilmini Mendis Philippe Cantin Geneviève Marchand Hugues Charest Frédéric Raymond Caroline Huot Isabelle Goupil-Sormany François Desbiens Sébastien P Faucher Jacques Corbeil Cécile Tremblay
author_facet	Simon Lévesque Pier-Luc Plante Nilmini Mendis Philippe Cantin Geneviève Marchand Hugues Charest Frédéric Raymond Caroline Huot Isabelle Goupil-Sormany François Desbiens Sébastien P Faucher Jacques Corbeil Cécile Tremblay
author_sort	Simon Lévesque
title	Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
title_short	Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
title_full	Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
title_fullStr	Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
title_full_unstemmed	Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.
title_sort	genomic characterization of a large outbreak of legionella pneumophila serogroup 1 strains in quebec city, 2012.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/426416e5e6024f829f201b02ae19ba92
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Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012.

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